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Direct detection of Mycobacterium tuberculosis and drug resistance in respiratory specimen using Abbott Realtime MTB detection and RIF/INH resistance assay.
Diagn Microbiol Infect Dis. 2017 Oct; 89(2):118-124.DM

Abstract

Abbott RealTime MTB (Abbott-RT) in conjunction with Abbott RealTime MTB RIF/INH Resistance (Abbott-RIF/INH) is a new, high-throughput automated nucleic acid amplification platform (Abbott-MDR) for detection of Mycobacterium tuberculosis complex (MTBC) and the genotypic markers for rifampicin (RIF) and isoniazid (INH) resistance directly from respiratory specimens. This prospective study evaluated the diagnostic performance of this new platform for MTBC and multidrug-resistant tuberculosis (MDR-TB) using 610 sputum specimens in a tuberculosis high-burden setting. Using conventional culture results and clinical background as reference standards, Abbott-RT exhibited an overall sensitivity and specificity of 95.2% and 99.8%, respectively. Genotypic RIF/INH resistance of 178 "MTB detected" specimens was subsequently analyzed by Abbott-RIF/INH. Compared to phenotypic drug susceptibility test results, Abbott-RIF/INH detected resistance genotypic markers in 84.6% MDR-TB, 80% mono-RIF-resistant and 66.7% mono-INH-resistant specimens. Two of the RIF-resistant specimens carried a novel single, nonsense mutation at rpoB Q513 and in silico simulation demonstrated that the truncated RpoB protein failed to bind with other subunits for transcription. Overall, Abbott-MDR platform provided high throughput and reliable diagnosis of MDR-TB within a TB high-burden region.

Authors+Show Affiliations

Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China.Department of Biomedical Sciences, City University of Hong Kong, Hong Kong Special Administrative Region, China.Department of Pathology, Kwong Wah Hospital, Hong Kong Special Administrative Region, China.Department of Pathology, Kwong Wah Hospital, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China. Electronic address: wcyam@hku.hk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28780247

Citation

Tam, Kingsley King-Gee, et al. "Direct Detection of Mycobacterium Tuberculosis and Drug Resistance in Respiratory Specimen Using Abbott Realtime MTB Detection and RIF/INH Resistance Assay." Diagnostic Microbiology and Infectious Disease, vol. 89, no. 2, 2017, pp. 118-124.
Tam KK, Leung KS, To SW, et al. Direct detection of Mycobacterium tuberculosis and drug resistance in respiratory specimen using Abbott Realtime MTB detection and RIF/INH resistance assay. Diagn Microbiol Infect Dis. 2017;89(2):118-124.
Tam, K. K., Leung, K. S., To, S. W., Siu, G. K., Lau, T. C., Shek, V. C., Tse, C. W., Wong, S. S., Ho, P. L., & Yam, W. C. (2017). Direct detection of Mycobacterium tuberculosis and drug resistance in respiratory specimen using Abbott Realtime MTB detection and RIF/INH resistance assay. Diagnostic Microbiology and Infectious Disease, 89(2), 118-124. https://doi.org/10.1016/j.diagmicrobio.2017.06.018
Tam KK, et al. Direct Detection of Mycobacterium Tuberculosis and Drug Resistance in Respiratory Specimen Using Abbott Realtime MTB Detection and RIF/INH Resistance Assay. Diagn Microbiol Infect Dis. 2017;89(2):118-124. PubMed PMID: 28780247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct detection of Mycobacterium tuberculosis and drug resistance in respiratory specimen using Abbott Realtime MTB detection and RIF/INH resistance assay. AU - Tam,Kingsley King-Gee, AU - Leung,Kenneth Siu-Sing, AU - To,Sabrina Wai-Chi, AU - Siu,Gilman Kit-Hang, AU - Lau,Terrence Chi-Kong, AU - Shek,Victor Chi-Man, AU - Tse,Cindy Wing-Sze, AU - Wong,Samson Sai-Yin, AU - Ho,Pak-Leung, AU - Yam,Wing-Cheong, Y1 - 2017/07/01/ PY - 2017/02/17/received PY - 2017/06/19/revised PY - 2017/06/20/accepted PY - 2017/8/7/pubmed PY - 2018/5/11/medline PY - 2017/8/7/entrez KW - Direct detection KW - Multidrug-resistant tuberculosis KW - Mycobacteriology KW - Mycobacterium tuberculosis KW - Respiratory specimen SP - 118 EP - 124 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 89 IS - 2 N2 - Abbott RealTime MTB (Abbott-RT) in conjunction with Abbott RealTime MTB RIF/INH Resistance (Abbott-RIF/INH) is a new, high-throughput automated nucleic acid amplification platform (Abbott-MDR) for detection of Mycobacterium tuberculosis complex (MTBC) and the genotypic markers for rifampicin (RIF) and isoniazid (INH) resistance directly from respiratory specimens. This prospective study evaluated the diagnostic performance of this new platform for MTBC and multidrug-resistant tuberculosis (MDR-TB) using 610 sputum specimens in a tuberculosis high-burden setting. Using conventional culture results and clinical background as reference standards, Abbott-RT exhibited an overall sensitivity and specificity of 95.2% and 99.8%, respectively. Genotypic RIF/INH resistance of 178 "MTB detected" specimens was subsequently analyzed by Abbott-RIF/INH. Compared to phenotypic drug susceptibility test results, Abbott-RIF/INH detected resistance genotypic markers in 84.6% MDR-TB, 80% mono-RIF-resistant and 66.7% mono-INH-resistant specimens. Two of the RIF-resistant specimens carried a novel single, nonsense mutation at rpoB Q513 and in silico simulation demonstrated that the truncated RpoB protein failed to bind with other subunits for transcription. Overall, Abbott-MDR platform provided high throughput and reliable diagnosis of MDR-TB within a TB high-burden region. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/28780247/Direct_detection_of_Mycobacterium_tuberculosis_and_drug_resistance_in_respiratory_specimen_using_Abbott_Realtime_MTB_detection_and_RIF/INH_resistance_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(17)30209-2 DB - PRIME DP - Unbound Medicine ER -