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Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity.
Molecules. 2017 Aug 07; 22(8)M

Abstract

In order to maximize the yield of the total phenolic content (TPC) and total monomeric anthocyanin (TMA) from Brosimum alicastrum leaf and to study the radical-scavenging activity, a three-level three-factor Box-Behnken design (BBD) was used to determine the optimal points for ultrasound-assisted extraction (UAE). In this study, we analyzed the extraction time (10, 20, and 30 min), temperature (28, 30, and 32 °C), and probe sonication power (40%, 28 W/cm²; 60%, 51 W/cm²; and 80%, 74 W/cm²). Analysis of variance (ANOVA) indicated that the sonication power plays a significant role in the extraction of phenolic compounds. An increase in time and temperature resulted in a decrease in the yield, in particular, of the TMA group. DPPH was found to be a better indicator of radical-scavenging activity than ABTS. The predicted TPC and TMA optimum levels (45.18 mg GAE/g and 15.16 mg CyE/100 g) were obtained at 28 °C, 80%, and 20-10 min. DPPH obtained a maximum value (67.27 μmol TE/g) under same optimization conditions. The RSM confirmed that TPC and TMA enhanced the antioxidant activity when subjected to low temperature (28 °C), extraction time less than 20 min, and higher sonication power (74 W/cm²), and hence achieving the better DPPH scavenging activity.

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Authors+Show Affiliations

Gullian Klanian M
Experimental Unit, University Marist of Mérida, Periférico Norte Tablaje Catastral 13941, Carretera Mérida-Progreso, 97300 Mérida, Yucatán, Mexico. mgullian@marista.edu.mx.
Terrats Preciat M
Experimental Unit, University Marist of Mérida, Periférico Norte Tablaje Catastral 13941, Carretera Mérida-Progreso, 97300 Mérida, Yucatán, Mexico. mterrats@marista.edu.mx.

MeSH

Free Radical ScavengersLiquid-Liquid ExtractionMoraceaePhenolsPhytochemicalsPlant LeavesUltrasonic Waves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28783112

Citation

Gullian Klanian, Mariel, and Montserrat Terrats Preciat. "Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds From Brosimum Alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity." Molecules (Basel, Switzerland), vol. 22, no. 8, 2017.
Gullian Klanian M, Terrats Preciat M. Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity. Molecules. 2017;22(8).
Gullian Klanian, M., & Terrats Preciat, M. (2017). Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity. Molecules (Basel, Switzerland), 22(8). https://doi.org/10.3390/molecules22081286
Gullian Klanian M, Terrats Preciat M. Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds From Brosimum Alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity. Molecules. 2017 Aug 7;22(8) PubMed PMID: 28783112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity. AU - Gullian Klanian,Mariel, AU - Terrats Preciat,Montserrat, Y1 - 2017/08/07/ PY - 2017/07/17/received PY - 2017/07/29/revised PY - 2017/07/31/accepted PY - 2017/8/8/entrez PY - 2017/8/8/pubmed PY - 2018/4/24/medline KW - Brosimum alicastrum leaf KW - monomeric anthocyanin KW - radical-scavenging activity KW - response surface methodology KW - total phenolic content KW - ultrasound assisted extraction JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 8 N2 - In order to maximize the yield of the total phenolic content (TPC) and total monomeric anthocyanin (TMA) from Brosimum alicastrum leaf and to study the radical-scavenging activity, a three-level three-factor Box-Behnken design (BBD) was used to determine the optimal points for ultrasound-assisted extraction (UAE). In this study, we analyzed the extraction time (10, 20, and 30 min), temperature (28, 30, and 32 °C), and probe sonication power (40%, 28 W/cm²; 60%, 51 W/cm²; and 80%, 74 W/cm²). Analysis of variance (ANOVA) indicated that the sonication power plays a significant role in the extraction of phenolic compounds. An increase in time and temperature resulted in a decrease in the yield, in particular, of the TMA group. DPPH was found to be a better indicator of radical-scavenging activity than ABTS. The predicted TPC and TMA optimum levels (45.18 mg GAE/g and 15.16 mg CyE/100 g) were obtained at 28 °C, 80%, and 20-10 min. DPPH obtained a maximum value (67.27 μmol TE/g) under same optimization conditions. The RSM confirmed that TPC and TMA enhanced the antioxidant activity when subjected to low temperature (28 °C), extraction time less than 20 min, and higher sonication power (74 W/cm²), and hence achieving the better DPPH scavenging activity. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/28783112/Optimization_of_the_Ultrasound_Assisted_Extraction_of_Phenolic_Compounds_from_Brosimum_alicastrum_Leaves_and_the_Evaluation_of_Their_Radical_Scavenging_Activity_ L2 - https://www.mdpi.com/resolver?pii=molecules22081286 DB - PRIME DP - Unbound Medicine ER -