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Comparative ecotoxicity of polystyrene nanoparticles in natural seawater and reconstituted seawater using the rotifer Brachionus plicatilis.
Ecotoxicol Environ Saf. 2017 Nov; 145:557-563.EE

Abstract

The impact of nanoplastics using model polystyrene nanoparticles (PS NPs), anionic (PS-COOH) and cationic (PS-NH2), has been investigated on the marine rotifer Brachionus plicatilis, a major component of marine zooplanktonic species. The role of different surface charges in affecting PS NP behaviour and toxicity has been considered in high ionic strength media. To this aim, the selected media were standardized reconstituted seawater (RSW) and natural sea water (NSW), the latter resembling more natural exposure scenarios. Hatched rotifer larvae were exposed for 24h and 48h to both PS NPs in the range of 0.5-50μg/ml using PS NP suspensions made in RSW and NSW. No effects on lethality upon exposure to anionic NPs were observed despite a clear gut retention was evident in all exposed rotifers. On the contrary, cationic NPs caused lethality to rotifer larvae but LC50 values resulted lower in rotifers exposed in RSW (LC50=2.75±0.67µg/ml) compared to those exposed in NSW (LC50=6.62±0.87µg/ml). PS NPs showed similar pattern of aggregation in both high ionic strength media (RSW and NSW) but while anionic NPs resulted in large microscale aggregates (Z-average 1109 ± 128nm and 998±67nm respectively), cationic NP aggregates were still in nano-size forms (93.99 ± 11.22nm and 108.3 ± 12.79nm). Both PDI and Z-potential of PS NPs slightly differed in the two media suggesting a role of their different surface charges in affecting their behaviour and stability. Our findings confirm the role of surface charges in nanoplastic behaviour in salt water media and provide a first evidence of a different toxicity in rotifers using artificial media (RSW) compared to natural one (NSW). Such evidence poses the question on how to select the best medium in standardized ecotoxicity assays in order to properly assess their hazard to marine life in natural environmental scenarios.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Manfra L
Institute for Environmental Protection and Research (ISPRA) Rome, Italy; Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn Naples, Italy. Electronic address: loredana.manfra@isprambiente.it.
Rotini A
Department of Biology, University Tor Vergata, Rome, Italy. Electronic address: alice.rotini@uniroma2.it.
Bergami E
Department of Physical, Earth and Environmental Sciences, University of Siena, Italy. Electronic address: bergami@student.unisi.it.
Grassi G
Department of Physical, Earth and Environmental Sciences, University of Siena, Italy. Electronic address: grassi@student.unisi.it.
Faleri C
Department of Life Sciences, University of Siena, Italy. Electronic address: faleric@unisi.it.
Corsi I
Department of Physical, Earth and Environmental Sciences, University of Siena, Italy. Electronic address: ilaria.corsi@unisi.it.

MeSH

AnimalsCationsEcotoxicologyLarvaLethal Dose 50Models, TheoreticalNanoparticlesPolystyrenesRotiferaSeawaterSurface PropertiesToxicity Tests, AcuteWater Pollutants, Chemical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28800530

Citation

Manfra, L, et al. "Comparative Ecotoxicity of Polystyrene Nanoparticles in Natural Seawater and Reconstituted Seawater Using the Rotifer Brachionus Plicatilis." Ecotoxicology and Environmental Safety, vol. 145, 2017, pp. 557-563.
Manfra L, Rotini A, Bergami E, et al. Comparative ecotoxicity of polystyrene nanoparticles in natural seawater and reconstituted seawater using the rotifer Brachionus plicatilis. Ecotoxicol Environ Saf. 2017;145:557-563.
Manfra, L., Rotini, A., Bergami, E., Grassi, G., Faleri, C., & Corsi, I. (2017). Comparative ecotoxicity of polystyrene nanoparticles in natural seawater and reconstituted seawater using the rotifer Brachionus plicatilis. Ecotoxicology and Environmental Safety, 145, 557-563. https://doi.org/10.1016/j.ecoenv.2017.07.068
Manfra L, et al. Comparative Ecotoxicity of Polystyrene Nanoparticles in Natural Seawater and Reconstituted Seawater Using the Rotifer Brachionus Plicatilis. Ecotoxicol Environ Saf. 2017;145:557-563. PubMed PMID: 28800530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative ecotoxicity of polystyrene nanoparticles in natural seawater and reconstituted seawater using the rotifer Brachionus plicatilis. AU - Manfra,L, AU - Rotini,A, AU - Bergami,E, AU - Grassi,G, AU - Faleri,C, AU - Corsi,I, Y1 - 2017/08/08/ PY - 2017/05/12/received PY - 2017/07/12/revised PY - 2017/07/28/accepted PY - 2017/8/12/pubmed PY - 2017/12/9/medline PY - 2017/8/12/entrez KW - Ecotoxicity KW - Nanoplastics KW - PS NP surface charge KW - Polystyrene KW - Rotifer KW - Suitable testing medium SP - 557 EP - 563 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 145 N2 - The impact of nanoplastics using model polystyrene nanoparticles (PS NPs), anionic (PS-COOH) and cationic (PS-NH2), has been investigated on the marine rotifer Brachionus plicatilis, a major component of marine zooplanktonic species. The role of different surface charges in affecting PS NP behaviour and toxicity has been considered in high ionic strength media. To this aim, the selected media were standardized reconstituted seawater (RSW) and natural sea water (NSW), the latter resembling more natural exposure scenarios. Hatched rotifer larvae were exposed for 24h and 48h to both PS NPs in the range of 0.5-50μg/ml using PS NP suspensions made in RSW and NSW. No effects on lethality upon exposure to anionic NPs were observed despite a clear gut retention was evident in all exposed rotifers. On the contrary, cationic NPs caused lethality to rotifer larvae but LC50 values resulted lower in rotifers exposed in RSW (LC50=2.75±0.67µg/ml) compared to those exposed in NSW (LC50=6.62±0.87µg/ml). PS NPs showed similar pattern of aggregation in both high ionic strength media (RSW and NSW) but while anionic NPs resulted in large microscale aggregates (Z-average 1109 ± 128nm and 998±67nm respectively), cationic NP aggregates were still in nano-size forms (93.99 ± 11.22nm and 108.3 ± 12.79nm). Both PDI and Z-potential of PS NPs slightly differed in the two media suggesting a role of their different surface charges in affecting their behaviour and stability. Our findings confirm the role of surface charges in nanoplastic behaviour in salt water media and provide a first evidence of a different toxicity in rotifers using artificial media (RSW) compared to natural one (NSW). Such evidence poses the question on how to select the best medium in standardized ecotoxicity assays in order to properly assess their hazard to marine life in natural environmental scenarios. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/28800530/Comparative_ecotoxicity_of_polystyrene_nanoparticles_in_natural_seawater_and_reconstituted_seawater_using_the_rotifer_Brachionus_plicatilis_ DB - PRIME DP - Unbound Medicine ER -