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Structural, antioxidant and antibacterial activities of polysaccharides extracted from olive leaves.
Int J Biol Macromol. 2018 Jan; 106:425-432.IJ

Abstract

In the present study, hot-water extraction procedure was used to recover polysaccharides from olive leaves. Primary structural characteristics were determined by nuclear magnetic resonance spectroscopy (1H NMR and 13C NMR), Fourier Transform-Infrared spectroscopy (FT-IR) and X-ray diffractometry analysis. Emulsifying capacity, Zeta (ζ) potential and differential scanning calorimetric (DSC) of olive leaf polysaccharides (OLP) were investigated. Antioxidant and antibacterial activities were then examined. Infrared spectroscopy data revealed that OLP were constituted of functional groups OH, CO and CH which were specific to polysaccharides. Results of ζ-potential showed that OLP possessed an anionic structure and exhibited donated electron capacities. OLP displayed strong DPPH-radical scavenging activity (IC 50=34.80μg/mL). They showed also important reducing power and β-carotene bleaching inhibition activities. Besides, OLP have attractive antibacterial activity against S.enterica and M.luteus with inhibition zones of 23.5 and 21.5mm, respectively.

Authors+Show Affiliations

Laboratoire d'Analyse, Valorisation et Sécuritédes aliments, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Université de Sfax, BP 1173, 3038 Sfax, Tunisia; Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Université de Sfax, BP 1173, 3038, Sfax, Tunisia.Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Université de Sfax, BP 1173, 3038 Sfax, Tunisia.Laboratoire d'Analyse, Valorisation et Sécuritédes aliments, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Université de Sfax, BP 1173, 3038 Sfax, Tunisia.Laboratoire d'Analyse, Valorisation et Sécuritédes aliments, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Université de Sfax, BP 1173, 3038 Sfax, Tunisia.Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Université de Sfax, BP 1173, 3038, Sfax, Tunisia. Electronic address: mohamed.bouaziz@fsg.rnu.tn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28802847

Citation

Khemakhem, Ibtihel, et al. "Structural, Antioxidant and Antibacterial Activities of Polysaccharides Extracted From Olive Leaves." International Journal of Biological Macromolecules, vol. 106, 2018, pp. 425-432.
Khemakhem I, Abdelhedi O, Trigui I, et al. Structural, antioxidant and antibacterial activities of polysaccharides extracted from olive leaves. Int J Biol Macromol. 2018;106:425-432.
Khemakhem, I., Abdelhedi, O., Trigui, I., Ayadi, M. A., & Bouaziz, M. (2018). Structural, antioxidant and antibacterial activities of polysaccharides extracted from olive leaves. International Journal of Biological Macromolecules, 106, 425-432. https://doi.org/10.1016/j.ijbiomac.2017.08.037
Khemakhem I, et al. Structural, Antioxidant and Antibacterial Activities of Polysaccharides Extracted From Olive Leaves. Int J Biol Macromol. 2018;106:425-432. PubMed PMID: 28802847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural, antioxidant and antibacterial activities of polysaccharides extracted from olive leaves. AU - Khemakhem,Ibtihel, AU - Abdelhedi,Ola, AU - Trigui,Ines, AU - Ayadi,Mohamed Ali, AU - Bouaziz,Mohamed, Y1 - 2017/08/10/ PY - 2017/06/14/received PY - 2017/07/18/revised PY - 2017/08/04/accepted PY - 2017/8/15/pubmed PY - 2018/7/10/medline PY - 2017/8/14/entrez KW - Antibacterial activity KW - Antioxidant activity KW - Olive leaves KW - Polysaccharides KW - Structural characteristics SP - 425 EP - 432 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 106 N2 - In the present study, hot-water extraction procedure was used to recover polysaccharides from olive leaves. Primary structural characteristics were determined by nuclear magnetic resonance spectroscopy (1H NMR and 13C NMR), Fourier Transform-Infrared spectroscopy (FT-IR) and X-ray diffractometry analysis. Emulsifying capacity, Zeta (ζ) potential and differential scanning calorimetric (DSC) of olive leaf polysaccharides (OLP) were investigated. Antioxidant and antibacterial activities were then examined. Infrared spectroscopy data revealed that OLP were constituted of functional groups OH, CO and CH which were specific to polysaccharides. Results of ζ-potential showed that OLP possessed an anionic structure and exhibited donated electron capacities. OLP displayed strong DPPH-radical scavenging activity (IC 50=34.80μg/mL). They showed also important reducing power and β-carotene bleaching inhibition activities. Besides, OLP have attractive antibacterial activity against S.enterica and M.luteus with inhibition zones of 23.5 and 21.5mm, respectively. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28802847/Structural_antioxidant_and_antibacterial_activities_of_polysaccharides_extracted_from_olive_leaves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)32141-4 DB - PRIME DP - Unbound Medicine ER -