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Nelumbo nucifera leaf extract treatment attenuated preneoplastic lesions and oxidative stress in the livers of diethylnitrosamine-treated rats.
Environ Toxicol 2017; 32(11):2327-2340ET

Abstract

Lotus (Nelumbo nucifera Gaertn) possesses antioxidant, hepatoprotective, and anticancer potential. This study determined the protective role of aqueous extract from Nelumbo nucifera leaves (NLE) against N-diethylnitrosamine (DEN)-induced oxidative stress and hepatocellular carcinogenesis in a sample of Sprague-Dawley rats. NLE was fed orally to rats in which hepatic carcinoma was induced with DEN for 12 weeks. Five groups of 12 rats each were used for the study: Group I (control group) rats received distilled water; Group II rats were induced with DEN; Group III rats were induced with DEN and cotreated with 0.5% NLE; Group IV rats were induced with DEN and cotreated with 1.0% NLE; and Group V rats were induced with DEN and cotreated with 2.0% NLE. Clinical chemistry, organ weight, inflammatory marker, protein expression, enzyme, and antioxidant analyses were conducted. NLE administration to rats resulted in significantly decreased levels of serum alanine aminotransferase, aspartate aminotransferase, and albumin, which is indicative of hepatocellular damage, compared with the control group. DEN-induced oxidative stress was inhibited by NLE and this inhibition was paralleled by decreased lipid peroxides and increased glutathione transferase, superoxide dismutase, catalase, and glutathione peroxidase activity in liver tissues. The status of nonenzymatic antioxidants, such as reduced glutathione, was also found to be increased in NLE-administered rats. Furthermore, NLE decreased tumor size, hepatic Rac1, PKCα, and GSTπ expressions compared with the DEN-only group. Thus, supplementation of NLE reduced the adverse changes that occur because of liver cancer. These results prove that NLE protects against liver carcinogenesis induced because of treatment with DEN through blocking lipid peroxidation, hepatic cell damage, and enhancing the antioxidant defense system.

Authors+Show Affiliations

Department of Ophthalmology, Kaohsiung Armed Forces General Hospital, Kaohsiung City, 802, Taiwan. Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung City, 402, Taiwan.Division of Gastroenterology, Kaohsiung Armed Forces General Hospital, Kaohsiung City, 802, Taiwan.Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung City, 402, Taiwan.Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung City, 402, Taiwan.Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung City, 402, Taiwan. Department of Medical Research, Chung Shan Medical University Hospital, Taichung City, 402, Taiwan.Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung City, 402, Taiwan. Department of Medical Research, Chung Shan Medical University Hospital, Taichung City, 402, Taiwan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28804948

Citation

Horng, Chi-Ting, et al. "Nelumbo Nucifera Leaf Extract Treatment Attenuated Preneoplastic Lesions and Oxidative Stress in the Livers of Diethylnitrosamine-treated Rats." Environmental Toxicology, vol. 32, no. 11, 2017, pp. 2327-2340.
Horng CT, Huang CW, Yang MY, et al. Nelumbo nucifera leaf extract treatment attenuated preneoplastic lesions and oxidative stress in the livers of diethylnitrosamine-treated rats. Environ Toxicol. 2017;32(11):2327-2340.
Horng, C. T., Huang, C. W., Yang, M. Y., Chen, T. H., Chang, Y. C., & Wang, C. J. (2017). Nelumbo nucifera leaf extract treatment attenuated preneoplastic lesions and oxidative stress in the livers of diethylnitrosamine-treated rats. Environmental Toxicology, 32(11), pp. 2327-2340. doi:10.1002/tox.22434.
Horng CT, et al. Nelumbo Nucifera Leaf Extract Treatment Attenuated Preneoplastic Lesions and Oxidative Stress in the Livers of Diethylnitrosamine-treated Rats. Environ Toxicol. 2017;32(11):2327-2340. PubMed PMID: 28804948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nelumbo nucifera leaf extract treatment attenuated preneoplastic lesions and oxidative stress in the livers of diethylnitrosamine-treated rats. AU - Horng,Chi-Ting, AU - Huang,Chien-Wei, AU - Yang,Mon-Yuan, AU - Chen,Tzu-Hsin, AU - Chang,Yun-Ching, AU - Wang,Chau-Jong, Y1 - 2017/08/14/ PY - 2016/12/16/received PY - 2017/04/26/revised PY - 2017/04/26/accepted PY - 2017/8/15/pubmed PY - 2017/11/29/medline PY - 2017/8/15/entrez KW - Nelumbo nucifera leaves KW - Rac1 KW - diethylnitrosamine KW - hepatocellular carcinoma KW - oxidative stress SP - 2327 EP - 2340 JF - Environmental toxicology JO - Environ. Toxicol. VL - 32 IS - 11 N2 - Lotus (Nelumbo nucifera Gaertn) possesses antioxidant, hepatoprotective, and anticancer potential. This study determined the protective role of aqueous extract from Nelumbo nucifera leaves (NLE) against N-diethylnitrosamine (DEN)-induced oxidative stress and hepatocellular carcinogenesis in a sample of Sprague-Dawley rats. NLE was fed orally to rats in which hepatic carcinoma was induced with DEN for 12 weeks. Five groups of 12 rats each were used for the study: Group I (control group) rats received distilled water; Group II rats were induced with DEN; Group III rats were induced with DEN and cotreated with 0.5% NLE; Group IV rats were induced with DEN and cotreated with 1.0% NLE; and Group V rats were induced with DEN and cotreated with 2.0% NLE. Clinical chemistry, organ weight, inflammatory marker, protein expression, enzyme, and antioxidant analyses were conducted. NLE administration to rats resulted in significantly decreased levels of serum alanine aminotransferase, aspartate aminotransferase, and albumin, which is indicative of hepatocellular damage, compared with the control group. DEN-induced oxidative stress was inhibited by NLE and this inhibition was paralleled by decreased lipid peroxides and increased glutathione transferase, superoxide dismutase, catalase, and glutathione peroxidase activity in liver tissues. The status of nonenzymatic antioxidants, such as reduced glutathione, was also found to be increased in NLE-administered rats. Furthermore, NLE decreased tumor size, hepatic Rac1, PKCα, and GSTπ expressions compared with the DEN-only group. Thus, supplementation of NLE reduced the adverse changes that occur because of liver cancer. These results prove that NLE protects against liver carcinogenesis induced because of treatment with DEN through blocking lipid peroxidation, hepatic cell damage, and enhancing the antioxidant defense system. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/28804948/Nelumbo_nucifera_leaf_extract_treatment_attenuated_preneoplastic_lesions_and_oxidative_stress_in_the_livers_of_diethylnitrosamine_treated_rats_ L2 - https://doi.org/10.1002/tox.22434 DB - PRIME DP - Unbound Medicine ER -