Tags

Type your tag names separated by a space and hit enter

Adherent-Invasive E. coli enhances colonic hypersensitivity and P2X receptors expression during post-infectious period.
Gut Microbes 2018; 9(1):26-37GM

Abstract

Irritable Bowel Syndrome (IBS) and Inflammatory Bowel Disease (IBD) are related gastrointestinal disorders characterized by abdominal pain associated with colonic hypersensitivity (CHS). Studies in humans have reported an abnormal colonization of Adherent-Invasive E. coli (AIEC) in the ileum of Crohn's disease (CD) patients associated with overexpression of the bacterial colonizing receptor CEACAM6. The aim of the present study was to investigate whether AIEC reference strain LF82 could induce intestinal impairment during infectious and/or post-infectious periods and subsequently the development of CHS. Transgenic mice overexpressing human CEACAM6 protein (TG) and their wild-type littermates were gavaged by CD-associated AIEC bacteria (reference strain LF82) or PBS for 3 d. Colonic hypersensitivity was assessed by colorectal distension (CRD) test during infectious (D4) and post-infectious periods (D21). Several markers of intestinal inflammation were monitored and the colonic expression of purinergic P2X receptors was quantified. At D4, an increased visceromotor response (VMR) to the CRD test was observed in TG mice infected with CD-associated AIEC LF82 in comparison with non-infected TG mice and persisted in a subgroup of infected animals at D21 after bacteria clearance. Increased VMR was associated with low-grade intestinal inflammation, increased intestinal permeability and expression of P2X 3, 4 and 7. This study shows that certain susceptible hosts infected with CD-associated AIEC bacteria can develop persistent CHS associated with low-grade inflammation and increased P2X receptors expression. Thus, CD-associated AIEC infection in CEACAM6 transgenic mice could be used as a novel post-infectious mouse model mimicking quiescent IBD with IBS-like symptoms such as visceral pain.

Authors+Show Affiliations

a Université Clermont Auvergne , Inserm U1107, NeuroDol, CHRN Auvergne, Clermont-Ferrand , France.a Université Clermont Auvergne , Inserm U1107, NeuroDol, CHRN Auvergne, Clermont-Ferrand , France.a Université Clermont Auvergne , Inserm U1107, NeuroDol, CHRN Auvergne, Clermont-Ferrand , France.a Université Clermont Auvergne , Inserm U1107, NeuroDol, CHRN Auvergne, Clermont-Ferrand , France.a Université Clermont Auvergne , Inserm U1107, NeuroDol, CHRN Auvergne, Clermont-Ferrand , France.b Université Clermont Auvergne , Inserm U1071, M2iSH, USC-INRA 2018, Clermont-Ferrand , France.a Université Clermont Auvergne , Inserm U1107, NeuroDol, CHRN Auvergne, Clermont-Ferrand , France.a Université Clermont Auvergne , Inserm U1107, NeuroDol, CHRN Auvergne, Clermont-Ferrand , France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28806140

Citation

Lashermes, Amandine, et al. "Adherent-Invasive E. Coli Enhances Colonic Hypersensitivity and P2X Receptors Expression During Post-infectious Period." Gut Microbes, vol. 9, no. 1, 2018, pp. 26-37.
Lashermes A, Boudieu L, Barbier J, et al. Adherent-Invasive E. coli enhances colonic hypersensitivity and P2X receptors expression during post-infectious period. Gut Microbes. 2018;9(1):26-37.
Lashermes, A., Boudieu, L., Barbier, J., Sion, B., Gelot, A., Barnich, N., ... Carvalho, F. A. (2018). Adherent-Invasive E. coli enhances colonic hypersensitivity and P2X receptors expression during post-infectious period. Gut Microbes, 9(1), pp. 26-37. doi:10.1080/19490976.2017.1361091.
Lashermes A, et al. Adherent-Invasive E. Coli Enhances Colonic Hypersensitivity and P2X Receptors Expression During Post-infectious Period. Gut Microbes. 2018 01 2;9(1):26-37. PubMed PMID: 28806140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adherent-Invasive E. coli enhances colonic hypersensitivity and P2X receptors expression during post-infectious period. AU - Lashermes,Amandine, AU - Boudieu,Ludivine, AU - Barbier,Julie, AU - Sion,Benoit, AU - Gelot,Agathe, AU - Barnich,Nicolas, AU - Ardid,Denis, AU - Carvalho,Frédéric Antonio, Y1 - 2017/10/04/ PY - 2017/8/15/pubmed PY - 2018/9/28/medline PY - 2017/8/15/entrez KW - Adherent invasive E. coli KW - P2X receptors KW - colonic hypersensitivity KW - intestinal permeability KW - visceral pain SP - 26 EP - 37 JF - Gut microbes JO - Gut Microbes VL - 9 IS - 1 N2 - Irritable Bowel Syndrome (IBS) and Inflammatory Bowel Disease (IBD) are related gastrointestinal disorders characterized by abdominal pain associated with colonic hypersensitivity (CHS). Studies in humans have reported an abnormal colonization of Adherent-Invasive E. coli (AIEC) in the ileum of Crohn's disease (CD) patients associated with overexpression of the bacterial colonizing receptor CEACAM6. The aim of the present study was to investigate whether AIEC reference strain LF82 could induce intestinal impairment during infectious and/or post-infectious periods and subsequently the development of CHS. Transgenic mice overexpressing human CEACAM6 protein (TG) and their wild-type littermates were gavaged by CD-associated AIEC bacteria (reference strain LF82) or PBS for 3 d. Colonic hypersensitivity was assessed by colorectal distension (CRD) test during infectious (D4) and post-infectious periods (D21). Several markers of intestinal inflammation were monitored and the colonic expression of purinergic P2X receptors was quantified. At D4, an increased visceromotor response (VMR) to the CRD test was observed in TG mice infected with CD-associated AIEC LF82 in comparison with non-infected TG mice and persisted in a subgroup of infected animals at D21 after bacteria clearance. Increased VMR was associated with low-grade intestinal inflammation, increased intestinal permeability and expression of P2X 3, 4 and 7. This study shows that certain susceptible hosts infected with CD-associated AIEC bacteria can develop persistent CHS associated with low-grade inflammation and increased P2X receptors expression. Thus, CD-associated AIEC infection in CEACAM6 transgenic mice could be used as a novel post-infectious mouse model mimicking quiescent IBD with IBS-like symptoms such as visceral pain. SN - 1949-0984 UR - https://www.unboundmedicine.com/medline/citation/28806140/Adherent_Invasive_E__coli_enhances_colonic_hypersensitivity_and_P2X_receptors_expression_during_post_infectious_period_ L2 - http://www.tandfonline.com/doi/full/10.1080/19490976.2017.1361091 DB - PRIME DP - Unbound Medicine ER -