Citation
Barco, Sebastiano, et al. "Volumetric Adsorptive Microsampling-liquid Chromatography Tandem Mass Spectrometry Assay for the Simultaneous Quantification of Four Antibiotics in Human Blood: Method Development, Validation and Comparison With Dried Blood Spot." Journal of Pharmaceutical and Biomedical Analysis, vol. 145, 2017, pp. 704-710.
Barco S, Castagnola E, Moscatelli A, et al. Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot. J Pharm Biomed Anal. 2017;145:704-710.
Barco, S., Castagnola, E., Moscatelli, A., Rudge, J., Tripodi, G., & Cangemi, G. (2017). Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot. Journal of Pharmaceutical and Biomedical Analysis, 145, pp. 704-710. doi:10.1016/j.jpba.2017.07.033.
Barco S, et al. Volumetric Adsorptive Microsampling-liquid Chromatography Tandem Mass Spectrometry Assay for the Simultaneous Quantification of Four Antibiotics in Human Blood: Method Development, Validation and Comparison With Dried Blood Spot. J Pharm Biomed Anal. 2017 Oct 25;145:704-710. PubMed PMID: 28806566.
TY - JOUR
T1 - Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot.
AU - Barco,Sebastiano,
AU - Castagnola,Elio,
AU - Moscatelli,Andrea,
AU - Rudge,James,
AU - Tripodi,Gino,
AU - Cangemi,Giuliana,
Y1 - 2017/08/02/
PY - 2017/03/22/received
PY - 2017/07/26/revised
PY - 2017/07/27/accepted
PY - 2017/8/15/pubmed
PY - 2018/2/1/medline
PY - 2017/8/15/entrez
KW - Antibiotics
KW - Dried blood spot
KW - Liquid chromatography
KW - Tandem mass spectrometry quantitative bioanalysis
KW - Volumetric absorptive microsampling
SP - 704
EP - 710
JF - Journal of pharmaceutical and biomedical analysis
JO - J Pharm Biomed Anal
VL - 145
N2 - In this paper we show the development and validation of a volumetric absorptive microsampling (VAMS™)-LC-MS/MS method for the simultaneous quantification of four antibiotics: piperacillin-tazobactam, meropenem, linezolid and ceftazidime in 10μL human blood. The novel VAMS-LC-MS/MS method has been compared with a dried blood spot (DBS)-based method in terms of impact of hematocrit (HCT) on accuracy, reproducibility, recovery and matrix effect. Antibiotics were extracted from VAMS and DBS by protein precipitation with methanol after a re-hydration step at 37°C for 10min. LC-MS/MS was carried out on a Thermo Scientific™ TSQ Quantum™ Access MAX triple quadrupole coupled to an Accela ™UHPLC system. The VAMS-LC-MS/MS method is selective, precise and reproducible. In contrast to DBS, it allows an accurate quantification without any HCT influence. It has been applied to samples derived from pediatric patients under therapy. VAMS is a valid alternative sampling strategy for the quantification of antibiotics and is valuable in support of clinical PK/PD studies and consequently therapeutic drug monitoring (TDM) in pediatrics.
SN - 1873-264X
UR - https://www.unboundmedicine.com/medline/citation/28806566/Volumetric_adsorptive_microsampling_liquid_chromatography_tandem_mass_spectrometry_assay_for_the_simultaneous_quantification_of_four_antibiotics_in_human_blood:_Method_development_validation_and_comparison_with_dried_blood_spot_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(17)30711-2
DB - PRIME
DP - Unbound Medicine
ER -