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Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot.
J Pharm Biomed Anal 2017; 145:704-710JP

Abstract

In this paper we show the development and validation of a volumetric absorptive microsampling (VAMS™)-LC-MS/MS method for the simultaneous quantification of four antibiotics: piperacillin-tazobactam, meropenem, linezolid and ceftazidime in 10μL human blood. The novel VAMS-LC-MS/MS method has been compared with a dried blood spot (DBS)-based method in terms of impact of hematocrit (HCT) on accuracy, reproducibility, recovery and matrix effect. Antibiotics were extracted from VAMS and DBS by protein precipitation with methanol after a re-hydration step at 37°C for 10min. LC-MS/MS was carried out on a Thermo Scientific™ TSQ Quantum™ Access MAX triple quadrupole coupled to an Accela ™UHPLC system. The VAMS-LC-MS/MS method is selective, precise and reproducible. In contrast to DBS, it allows an accurate quantification without any HCT influence. It has been applied to samples derived from pediatric patients under therapy. VAMS is a valid alternative sampling strategy for the quantification of antibiotics and is valuable in support of clinical PK/PD studies and consequently therapeutic drug monitoring (TDM) in pediatrics.

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    Authors+Show Affiliations

    Barco S
    Clinical Pahology Laboratory Unit, Istituto Giannina Gaslini, Via G. Gaslini 5, 16147 Genoa, Italy.
    Castagnola E
    Infectious Disease Unit, Istituto Giannina Gaslini, Via G. Gaslini 5, 16147 Genoa, Italy.
    Moscatelli A
    Intensive Care Unit, Istituto Giannina Gaslini, Via G.Gaslini 5, 16147 Genoa, Italy.
    Rudge J
    Neoteryx LLC, 421 Amapola Avenue, 90501 Torrance CA, USA.
    Tripodi G
    Clinical Pahology Laboratory Unit, Istituto Giannina Gaslini, Via G. Gaslini 5, 16147 Genoa, Italy.
    Cangemi G
    Clinical Pahology Laboratory Unit, Istituto Giannina Gaslini, Via G. Gaslini 5, 16147 Genoa, Italy. Electronic address: giulianacangemi@gaslini.org.

    MeSH

    Anti-Bacterial AgentsChromatography, LiquidDried Blood Spot TestingHumansReproducibility of ResultsTandem Mass Spectrometry

    Pub Type(s)

    Comparative Study
    Journal Article
    Validation Studies

    Language

    eng

    PubMed ID

    28806566

    Citation

    Barco, Sebastiano, et al. "Volumetric Adsorptive Microsampling-liquid Chromatography Tandem Mass Spectrometry Assay for the Simultaneous Quantification of Four Antibiotics in Human Blood: Method Development, Validation and Comparison With Dried Blood Spot." Journal of Pharmaceutical and Biomedical Analysis, vol. 145, 2017, pp. 704-710.
    Barco S, Castagnola E, Moscatelli A, et al. Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot. J Pharm Biomed Anal. 2017;145:704-710.
    Barco, S., Castagnola, E., Moscatelli, A., Rudge, J., Tripodi, G., & Cangemi, G. (2017). Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot. Journal of Pharmaceutical and Biomedical Analysis, 145, pp. 704-710. doi:10.1016/j.jpba.2017.07.033.
    Barco S, et al. Volumetric Adsorptive Microsampling-liquid Chromatography Tandem Mass Spectrometry Assay for the Simultaneous Quantification of Four Antibiotics in Human Blood: Method Development, Validation and Comparison With Dried Blood Spot. J Pharm Biomed Anal. 2017 Oct 25;145:704-710. PubMed PMID: 28806566.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot. AU - Barco,Sebastiano, AU - Castagnola,Elio, AU - Moscatelli,Andrea, AU - Rudge,James, AU - Tripodi,Gino, AU - Cangemi,Giuliana, Y1 - 2017/08/02/ PY - 2017/03/22/received PY - 2017/07/26/revised PY - 2017/07/27/accepted PY - 2017/8/15/pubmed PY - 2018/2/1/medline PY - 2017/8/15/entrez KW - Antibiotics KW - Dried blood spot KW - Liquid chromatography KW - Tandem mass spectrometry quantitative bioanalysis KW - Volumetric absorptive microsampling SP - 704 EP - 710 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 145 N2 - In this paper we show the development and validation of a volumetric absorptive microsampling (VAMS™)-LC-MS/MS method for the simultaneous quantification of four antibiotics: piperacillin-tazobactam, meropenem, linezolid and ceftazidime in 10μL human blood. The novel VAMS-LC-MS/MS method has been compared with a dried blood spot (DBS)-based method in terms of impact of hematocrit (HCT) on accuracy, reproducibility, recovery and matrix effect. Antibiotics were extracted from VAMS and DBS by protein precipitation with methanol after a re-hydration step at 37°C for 10min. LC-MS/MS was carried out on a Thermo Scientific™ TSQ Quantum™ Access MAX triple quadrupole coupled to an Accela ™UHPLC system. The VAMS-LC-MS/MS method is selective, precise and reproducible. In contrast to DBS, it allows an accurate quantification without any HCT influence. It has been applied to samples derived from pediatric patients under therapy. VAMS is a valid alternative sampling strategy for the quantification of antibiotics and is valuable in support of clinical PK/PD studies and consequently therapeutic drug monitoring (TDM) in pediatrics. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/28806566/Volumetric_adsorptive_microsampling_liquid_chromatography_tandem_mass_spectrometry_assay_for_the_simultaneous_quantification_of_four_antibiotics_in_human_blood:_Method_development_validation_and_comparison_with_dried_blood_spot_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(17)30711-2 DB - PRIME DP - Unbound Medicine ER -