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Protective effects of orange (Citrus sinensis L.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat.
Lipids Health Dis. 2017 Aug 14; 16(1):152.LH

Abstract

BACKGROUND

Massive alcohol drinking can lead to gastric ulcer. In the present study we investigated the gastroprotective effect of Citrus sinensis peel aqueous extract (CSPE) and Hesperidin (H) in ethanol (EtOH) induced oxidative stress and peptic ulcer in rats.

METHODS

Seventy adult male Wistar rats were divided into seven groups of 10 each: control, EtOH (4 g/kg b.w.), EtOH + various doses of CSPE (100, 200 and 400 mg/kg, b.w.), EtOH + Hesperidin (50 mg/kg, p.o.) and EtOH + Omeprazole (OM, 20 mg/kg, p.o.). Animals were perorally (p.o.) pre-treated with CSPE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) during 2 h. Gastric ulcer was induced in rats with a single dose of ethanol (EtOH). Ulcer index, gene expression of gastric cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), malondialdhyde (MDA), hydrogen peroxide H2O2 and Thiol groups (-SH) content in stomach and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and gluthation peroxidise (GPx) were measured. Furthermore, histopathological examinations were performed.

RESULTS

The results showed that ethanol induced gastric damage, improving oxidative stress markers level such as MDA (121 ± 4.45 nmol/mg proteins) and H2O2 (24.62 ± 1.04 μmol/mg proteins), increased pro-inflammatory cytokine (TNF-α level), as well as the expression of COX-2 in the ethanol group. However, a significant depletion of enzymatic and non-enzymatic antioxidants were observed, such as, GPx (72%), SOD (57.5%), CAT (41.6%) and -SH (50%). The lesions were associated with severe histopathological damage. The both Citrus sinensis peel aqueous extract (CSPE) and hesperidin significantly protect against all gastric damages caused by ethanol administration in rats.

CONCLUSIONS

We propose that CSPE and hesperidin exhibit protective effects in EtOH-induced peptic ulcer in rat. This protection might be related in to part its antioxidant properties as well as its opposite effects on some studied intracellular mediators.

Authors+Show Affiliations

Higher Institute of Biotechnology of Beja, Laboratory Functional Physiology and Bio-resources Valorisation, University of Jendouba, Avenue Habib Bourguiba, BP, 382, 9000, Beja, Tunisia. slimen.selmi@gmail.com.Higher Institute of Biotechnology of Beja, Laboratory Functional Physiology and Bio-resources Valorisation, University of Jendouba, Avenue Habib Bourguiba, BP, 382, 9000, Beja, Tunisia.Higher Institute of Biotechnology of Beja, Laboratory Functional Physiology and Bio-resources Valorisation, University of Jendouba, Avenue Habib Bourguiba, BP, 382, 9000, Beja, Tunisia.Higher Institute of Biotechnology of Beja, Laboratory Functional Physiology and Bio-resources Valorisation, University of Jendouba, Avenue Habib Bourguiba, BP, 382, 9000, Beja, Tunisia.Higher Institute of Biotechnology of Beja, Laboratory Functional Physiology and Bio-resources Valorisation, University of Jendouba, Avenue Habib Bourguiba, BP, 382, 9000, Beja, Tunisia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28806973

Citation

Selmi, Slimen, et al. "Protective Effects of Orange (Citrus Sinensis L.) Peel Aqueous Extract and Hesperidin On Oxidative Stress and Peptic Ulcer Induced By Alcohol in Rat." Lipids in Health and Disease, vol. 16, no. 1, 2017, p. 152.
Selmi S, Rtibi K, Grami D, et al. Protective effects of orange (Citrus sinensis L.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat. Lipids Health Dis. 2017;16(1):152.
Selmi, S., Rtibi, K., Grami, D., Sebai, H., & Marzouki, L. (2017). Protective effects of orange (Citrus sinensis L.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat. Lipids in Health and Disease, 16(1), 152. https://doi.org/10.1186/s12944-017-0546-y
Selmi S, et al. Protective Effects of Orange (Citrus Sinensis L.) Peel Aqueous Extract and Hesperidin On Oxidative Stress and Peptic Ulcer Induced By Alcohol in Rat. Lipids Health Dis. 2017 Aug 14;16(1):152. PubMed PMID: 28806973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of orange (Citrus sinensis L.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat. AU - Selmi,Slimen, AU - Rtibi,Kais, AU - Grami,Dhekra, AU - Sebai,Hichem, AU - Marzouki,Lamjed, Y1 - 2017/08/14/ PY - 2017/07/03/received PY - 2017/08/02/accepted PY - 2017/8/16/entrez PY - 2017/8/16/pubmed PY - 2018/5/1/medline KW - COX-2 KW - Citrus sinensis peel KW - Gastric ulcer KW - Hesperidin KW - Oxidative stress markers KW - Rat KW - TNF-α SP - 152 EP - 152 JF - Lipids in health and disease JO - Lipids Health Dis VL - 16 IS - 1 N2 - BACKGROUND: Massive alcohol drinking can lead to gastric ulcer. In the present study we investigated the gastroprotective effect of Citrus sinensis peel aqueous extract (CSPE) and Hesperidin (H) in ethanol (EtOH) induced oxidative stress and peptic ulcer in rats. METHODS: Seventy adult male Wistar rats were divided into seven groups of 10 each: control, EtOH (4 g/kg b.w.), EtOH + various doses of CSPE (100, 200 and 400 mg/kg, b.w.), EtOH + Hesperidin (50 mg/kg, p.o.) and EtOH + Omeprazole (OM, 20 mg/kg, p.o.). Animals were perorally (p.o.) pre-treated with CSPE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) during 2 h. Gastric ulcer was induced in rats with a single dose of ethanol (EtOH). Ulcer index, gene expression of gastric cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), malondialdhyde (MDA), hydrogen peroxide H2O2 and Thiol groups (-SH) content in stomach and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and gluthation peroxidise (GPx) were measured. Furthermore, histopathological examinations were performed. RESULTS: The results showed that ethanol induced gastric damage, improving oxidative stress markers level such as MDA (121 ± 4.45 nmol/mg proteins) and H2O2 (24.62 ± 1.04 μmol/mg proteins), increased pro-inflammatory cytokine (TNF-α level), as well as the expression of COX-2 in the ethanol group. However, a significant depletion of enzymatic and non-enzymatic antioxidants were observed, such as, GPx (72%), SOD (57.5%), CAT (41.6%) and -SH (50%). The lesions were associated with severe histopathological damage. The both Citrus sinensis peel aqueous extract (CSPE) and hesperidin significantly protect against all gastric damages caused by ethanol administration in rats. CONCLUSIONS: We propose that CSPE and hesperidin exhibit protective effects in EtOH-induced peptic ulcer in rat. This protection might be related in to part its antioxidant properties as well as its opposite effects on some studied intracellular mediators. SN - 1476-511X UR - https://www.unboundmedicine.com/medline/citation/28806973/Protective_effects_of_orange__Citrus_sinensis_L___peel_aqueous_extract_and_hesperidin_on_oxidative_stress_and_peptic_ulcer_induced_by_alcohol_in_rat_ L2 - https://lipidworld.biomedcentral.com/articles/10.1186/s12944-017-0546-y DB - PRIME DP - Unbound Medicine ER -