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The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment.
Microbiome. 2017 08 14; 5(1):99.M

Abstract

BACKGROUND

The urinary microbiota is similarly complex as the vaginal and penile microbiota, yet its role as a reservoir for pathogens and for recurrent polymicrobial biofilm diseases like bacterial vaginosis (BV) is not clear.

RESULTS

Here, we analysed the urinary microbiota of healthy men and women and compared it with that of women during BV and after antibiotic treatment using next-generation sequencing of the 16S rRNA gene V1-V2 regions. Eight different community types, so called urotypes (UT), were identified in healthy humans, all of which were shared between men and women, except UT 7, dominated in relative abundance by Lactobacillus crispatus, which was found in healthy women only. Orally applied metronidazole significantly reduced Shannon diversity and the mean relative abundance of Gardnerella vaginalis, Atopobium vaginae, and Sneathia amnii, while L. iners increased to levels twofold higher than those found in healthy women. Although individual urine microbial profiles strongly responded to the antibiotic, the healthy community could not be restored. The correlation between urinary and vaginal fluid microbiota was generally weak and depending on UT and BV status. It was highest in UT 1 in acute BV (59% of samples), but after metronidazole treatment, only 3 out of 35 women showed a significant correlation between their urinary and vaginal microbiota composition.

CONCLUSIONS

Urethra and bladder thus harbor microbial communities distinct from the vagina. The high abundance of BV related species in the urine of both men and women suggests that urine may act as a reservoir of pathogens and contribute to recurrence.

TRIAL REGISTRATION

ClinicalTrials.gov, NCT02687789.

Authors+Show Affiliations

Research Group Microbial Communication, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.Research Group Microbial Communication, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.Dr. August Wolff GmbH & Co. KG Arzneimittel, Sudbrackstrasse 56, 33611, Bielefeld, Germany.Dr. August Wolff GmbH & Co. KG Arzneimittel, Sudbrackstrasse 56, 33611, Bielefeld, Germany.Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.Research Group Microbial Communication, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany. Irene.Wagner-Doebler@helmholtz-hzi.de.

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28807017

Citation

Gottschick, Cornelia, et al. "The Urinary Microbiota of Men and Women and Its Changes in Women During Bacterial Vaginosis and Antibiotic Treatment." Microbiome, vol. 5, no. 1, 2017, p. 99.
Gottschick C, Deng ZL, Vital M, et al. The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment. Microbiome. 2017;5(1):99.
Gottschick, C., Deng, Z. L., Vital, M., Masur, C., Abels, C., Pieper, D. H., & Wagner-Döbler, I. (2017). The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment. Microbiome, 5(1), 99. https://doi.org/10.1186/s40168-017-0305-3
Gottschick C, et al. The Urinary Microbiota of Men and Women and Its Changes in Women During Bacterial Vaginosis and Antibiotic Treatment. Microbiome. 2017 08 14;5(1):99. PubMed PMID: 28807017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment. AU - Gottschick,Cornelia, AU - Deng,Zhi-Luo, AU - Vital,Marius, AU - Masur,Clarissa, AU - Abels,Christoph, AU - Pieper,Dietmar H, AU - Wagner-Döbler,Irene, Y1 - 2017/08/14/ PY - 2017/03/02/received PY - 2017/07/12/accepted PY - 2017/8/16/entrez PY - 2017/8/16/pubmed PY - 2018/3/15/medline KW - Antibiotic treatment KW - Bacterial vaginosis KW - Healthy urinary microbiome KW - Urinary microbiota KW - Urotypes KW - Vaginal microbiota SP - 99 EP - 99 JF - Microbiome JO - Microbiome VL - 5 IS - 1 N2 - BACKGROUND: The urinary microbiota is similarly complex as the vaginal and penile microbiota, yet its role as a reservoir for pathogens and for recurrent polymicrobial biofilm diseases like bacterial vaginosis (BV) is not clear. RESULTS: Here, we analysed the urinary microbiota of healthy men and women and compared it with that of women during BV and after antibiotic treatment using next-generation sequencing of the 16S rRNA gene V1-V2 regions. Eight different community types, so called urotypes (UT), were identified in healthy humans, all of which were shared between men and women, except UT 7, dominated in relative abundance by Lactobacillus crispatus, which was found in healthy women only. Orally applied metronidazole significantly reduced Shannon diversity and the mean relative abundance of Gardnerella vaginalis, Atopobium vaginae, and Sneathia amnii, while L. iners increased to levels twofold higher than those found in healthy women. Although individual urine microbial profiles strongly responded to the antibiotic, the healthy community could not be restored. The correlation between urinary and vaginal fluid microbiota was generally weak and depending on UT and BV status. It was highest in UT 1 in acute BV (59% of samples), but after metronidazole treatment, only 3 out of 35 women showed a significant correlation between their urinary and vaginal microbiota composition. CONCLUSIONS: Urethra and bladder thus harbor microbial communities distinct from the vagina. The high abundance of BV related species in the urine of both men and women suggests that urine may act as a reservoir of pathogens and contribute to recurrence. TRIAL REGISTRATION: ClinicalTrials.gov, NCT02687789. SN - 2049-2618 UR - https://www.unboundmedicine.com/medline/citation/28807017/The_urinary_microbiota_of_men_and_women_and_its_changes_in_women_during_bacterial_vaginosis_and_antibiotic_treatment_ L2 - https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0305-3 DB - PRIME DP - Unbound Medicine ER -