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Identification and quantification and antioxidant activity of flavonoids in different strains of silk cocoon, Bombyx mori.
Arch Biochem Biophys. 2017 10 01; 631:58-65.AB

Abstract

Silk cocoon is produced from silkworm (Bombyx mori) to protect itself from outer environment. Various strains of cocoon contain different forms and amounts of flavonoids, which may affect on their antioxidant activity. Moreover, the extraction method would influence the amount of flavonoids extracted. Therefore, the objectives of this study were to identify and quantify the flavonoids in 3 strains of bivoltine Bombyx mori silk cocoon (Chul 1/1; white cocoon, Chul 3/2; greenish cocoon, and Chul 4/2; yellow cocoon) extracted by 6 different solvents including acetone, ethyl acetate, dimethyl sulfoxide (DMSO), ethanol, methanol, and purified water. The flavonoids extracted were identified and quantified by liquid chromatography-mass spectrometry (LC-MS). The antioxidant activity of flavonoids extracted was also investigated by visible spectroscopy at 517 nm. The results showed that Chul 3/2 silk cocoon contained the highest amount of flavonoids. Purified water seemed to be the best solvent that preserved most antioxidant activity of the flavonoids extracted. Flavonoids in Chul 1/1 and Chul 4/2 silk cocoon were rarely found, however they contained some antioxidant activities. The data from this study can provide basic information for flavonoid extraction from silk cocoon which can also apply for other flavonoid-containing natural biomaterials.

Authors+Show Affiliations

Bioactive Resources for Innovative Clinical Applications Research Unit, Chulalongkorn University, Phatumwan, Bangkok 10330, Thailand; Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand.Austrian Drug Screening Institute (ADSI), University of Innsbruck, Innrain 66a, Innsbruck, 6020, Austria.Austrian Drug Screening Institute (ADSI), University of Innsbruck, Innrain 66a, Innsbruck, 6020, Austria.Austrian Drug Screening Institute (ADSI), University of Innsbruck, Innrain 66a, Innsbruck, 6020, Austria.Austrian Drug Screening Institute (ADSI), University of Innsbruck, Innrain 66a, Innsbruck, 6020, Austria.Bioactive Resources for Innovative Clinical Applications Research Unit, Chulalongkorn University, Phatumwan, Bangkok 10330, Thailand; Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand. Electronic address: aramwit@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28807613

Citation

Napavichayanun, Supamas, et al. "Identification and Quantification and Antioxidant Activity of Flavonoids in Different Strains of Silk Cocoon, Bombyx Mori." Archives of Biochemistry and Biophysics, vol. 631, 2017, pp. 58-65.
Napavichayanun S, Lutz O, Fischnaller M, et al. Identification and quantification and antioxidant activity of flavonoids in different strains of silk cocoon, Bombyx mori. Arch Biochem Biophys. 2017;631:58-65.
Napavichayanun, S., Lutz, O., Fischnaller, M., Jakschitz, T., Bonn, G., & Aramwit, P. (2017). Identification and quantification and antioxidant activity of flavonoids in different strains of silk cocoon, Bombyx mori. Archives of Biochemistry and Biophysics, 631, 58-65. https://doi.org/10.1016/j.abb.2017.08.010
Napavichayanun S, et al. Identification and Quantification and Antioxidant Activity of Flavonoids in Different Strains of Silk Cocoon, Bombyx Mori. Arch Biochem Biophys. 2017 10 1;631:58-65. PubMed PMID: 28807613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and quantification and antioxidant activity of flavonoids in different strains of silk cocoon, Bombyx mori. AU - Napavichayanun,Supamas, AU - Lutz,Oliver, AU - Fischnaller,Martin, AU - Jakschitz,Thomas, AU - Bonn,Günther, AU - Aramwit,Pornanong, Y1 - 2017/08/12/ PY - 2017/05/31/received PY - 2017/08/07/revised PY - 2017/08/09/accepted PY - 2017/8/16/pubmed PY - 2017/9/20/medline PY - 2017/8/16/entrez KW - Antioxidant activity KW - Flavonoid KW - Kaempferol KW - Quercetin KW - Silk cocoon SP - 58 EP - 65 JF - Archives of biochemistry and biophysics JO - Arch Biochem Biophys VL - 631 N2 - Silk cocoon is produced from silkworm (Bombyx mori) to protect itself from outer environment. Various strains of cocoon contain different forms and amounts of flavonoids, which may affect on their antioxidant activity. Moreover, the extraction method would influence the amount of flavonoids extracted. Therefore, the objectives of this study were to identify and quantify the flavonoids in 3 strains of bivoltine Bombyx mori silk cocoon (Chul 1/1; white cocoon, Chul 3/2; greenish cocoon, and Chul 4/2; yellow cocoon) extracted by 6 different solvents including acetone, ethyl acetate, dimethyl sulfoxide (DMSO), ethanol, methanol, and purified water. The flavonoids extracted were identified and quantified by liquid chromatography-mass spectrometry (LC-MS). The antioxidant activity of flavonoids extracted was also investigated by visible spectroscopy at 517 nm. The results showed that Chul 3/2 silk cocoon contained the highest amount of flavonoids. Purified water seemed to be the best solvent that preserved most antioxidant activity of the flavonoids extracted. Flavonoids in Chul 1/1 and Chul 4/2 silk cocoon were rarely found, however they contained some antioxidant activities. The data from this study can provide basic information for flavonoid extraction from silk cocoon which can also apply for other flavonoid-containing natural biomaterials. SN - 1096-0384 UR - https://www.unboundmedicine.com/medline/citation/28807613/Identification_and_quantification_and_antioxidant_activity_of_flavonoids_in_different_strains_of_silk_cocoon_Bombyx_mori_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9861(17)30342-9 DB - PRIME DP - Unbound Medicine ER -