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Aqueous extract from pecan nut [Carya illinoinensis (Wangenh) C. Koch] shell show activity against breast cancer cell line MCF-7 and Ehrlich ascites tumor in Balb-C mice.
J Ethnopharmacol. 2018 Jan 30; 211:256-266.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

In Brazil many health disorders are treated with the consumption of different varieties of tea. Shell extracts of pecan nut (Carya illinoinensis), which have significant amounts of phenolic compounds in their composition, are popularly taken as tea to prevent diverse pathologies.

AIM OF THE STUDY

Phenolic compounds from pecan nut shell extract have been associated with diverse biological effects but the effect on tumor cells has not been reported yet. The aim of the current work was to evaluate the relationship between DNA fragmentation, cell cycle arrest and apoptosis induced by pecan nut shell extract and its antitumor activity.

MATERIALS AND METHODS

Cytotoxicity, proliferation, cell death and cell cycle were evaluated in MCF-7 cells by MTT, colony assay, differential coloring and flow cytometry assays, respectively. DNA damage effects were evaluated through intercalation into CT-DNA and plasmid DNA cleavage. Tumor growth inhibition, survival time increase, apoptosis and cell cycle arrest were assessed in Ehrlich ascites tumor in Balb/C mice.

RESULTS

The cytotoxic effect of pecan nut shell extracts, the induction of cell death by apoptosis and also the cell cycle arrest in MCF-7 cells have been demonstrated. The survival time in mice with Ehrlich ascites tumor increased by 67%. DNA damage was observed in the CT-DNA, plasmid DNA and comet assays. The mechanism involved in the antitumor effect of pecan nut shell extracts may be related to the activation of key proteins involved in apoptosis cell death (Bcl-XL, Bax and p53) and on the cell cycle regulation (cyclin A, cyclin B and CDK2).

CONCLUSIONS

These results were attributed to the phenolic profile of the extract, which presented compounds such as gallic, 4-hydroxybenzoic, chlorogenic, vanillic, caffeic and ellagic acid, and catechin, epicatechin, epigallocatechin and epicatechin gallate. The results indicated that pecan nut shell extracts are effective against tumor cells growth and may be considered as an alternative to the treatment of cancer.

Authors+Show Affiliations

Laboratory of Fats and Oils, Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: josiane_hilbig@terra.com.br.Laboratory of Fats and Oils, Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: pripolicarpi@hotmail.com.Laboratory of Experimental Biochemistry, Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: valgrinev@gmail.com.Laboratory of Experimental Biochemistry, Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: nadiamota18@hotmail.com.Laboratory of Food Biochemistry, Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: bela.toaldo@gmail.com.Laboratory of Food Biochemistry, Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: marilde.bordignon@ufsc.br.Laboratory of Experimental Biochemistry, Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: rozangelapedrosa@gmail.com.Laboratory of Fats and Oils, Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: janeblock@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28807853

Citation

Hilbig, Josiane, et al. "Aqueous Extract From Pecan Nut [Carya Illinoinensis (Wangenh) C. Koch] Shell Show Activity Against Breast Cancer Cell Line MCF-7 and Ehrlich Ascites Tumor in Balb-C Mice." Journal of Ethnopharmacology, vol. 211, 2018, pp. 256-266.
Hilbig J, Policarpi PB, Grinevicius VMAS, et al. Aqueous extract from pecan nut [Carya illinoinensis (Wangenh) C. Koch] shell show activity against breast cancer cell line MCF-7 and Ehrlich ascites tumor in Balb-C mice. J Ethnopharmacol. 2018;211:256-266.
Hilbig, J., Policarpi, P. B., Grinevicius, V. M. A. S., Mota, N. S. R. S., Toaldo, I. M., Luiz, M. T. B., Pedrosa, R. C., & Block, J. M. (2018). Aqueous extract from pecan nut [Carya illinoinensis (Wangenh) C. Koch] shell show activity against breast cancer cell line MCF-7 and Ehrlich ascites tumor in Balb-C mice. Journal of Ethnopharmacology, 211, 256-266. https://doi.org/10.1016/j.jep.2017.08.012
Hilbig J, et al. Aqueous Extract From Pecan Nut [Carya Illinoinensis (Wangenh) C. Koch] Shell Show Activity Against Breast Cancer Cell Line MCF-7 and Ehrlich Ascites Tumor in Balb-C Mice. J Ethnopharmacol. 2018 Jan 30;211:256-266. PubMed PMID: 28807853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aqueous extract from pecan nut [Carya illinoinensis (Wangenh) C. Koch] shell show activity against breast cancer cell line MCF-7 and Ehrlich ascites tumor in Balb-C mice. AU - Hilbig,Josiane, AU - Policarpi,Priscila de Britto, AU - Grinevicius,Valdelúcia Maria Alves de Souza, AU - Mota,Nádia Sandrine Ramos Santos, AU - Toaldo,Isabela Maia, AU - Luiz,Marilde Terezinha Bordignon, AU - Pedrosa,Rozangela Curi, AU - Block,Jane Mara, Y1 - 2017/08/12/ PY - 2017/05/08/received PY - 2017/07/31/revised PY - 2017/08/09/accepted PY - 2017/8/16/pubmed PY - 2018/7/22/medline PY - 2017/8/16/entrez KW - Antitumor effects KW - Apoptosis KW - MCF-7 breast cancer cell KW - Pecan nut shell KW - Phenolic compounds SP - 256 EP - 266 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 211 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: In Brazil many health disorders are treated with the consumption of different varieties of tea. Shell extracts of pecan nut (Carya illinoinensis), which have significant amounts of phenolic compounds in their composition, are popularly taken as tea to prevent diverse pathologies. AIM OF THE STUDY: Phenolic compounds from pecan nut shell extract have been associated with diverse biological effects but the effect on tumor cells has not been reported yet. The aim of the current work was to evaluate the relationship between DNA fragmentation, cell cycle arrest and apoptosis induced by pecan nut shell extract and its antitumor activity. MATERIALS AND METHODS: Cytotoxicity, proliferation, cell death and cell cycle were evaluated in MCF-7 cells by MTT, colony assay, differential coloring and flow cytometry assays, respectively. DNA damage effects were evaluated through intercalation into CT-DNA and plasmid DNA cleavage. Tumor growth inhibition, survival time increase, apoptosis and cell cycle arrest were assessed in Ehrlich ascites tumor in Balb/C mice. RESULTS: The cytotoxic effect of pecan nut shell extracts, the induction of cell death by apoptosis and also the cell cycle arrest in MCF-7 cells have been demonstrated. The survival time in mice with Ehrlich ascites tumor increased by 67%. DNA damage was observed in the CT-DNA, plasmid DNA and comet assays. The mechanism involved in the antitumor effect of pecan nut shell extracts may be related to the activation of key proteins involved in apoptosis cell death (Bcl-XL, Bax and p53) and on the cell cycle regulation (cyclin A, cyclin B and CDK2). CONCLUSIONS: These results were attributed to the phenolic profile of the extract, which presented compounds such as gallic, 4-hydroxybenzoic, chlorogenic, vanillic, caffeic and ellagic acid, and catechin, epicatechin, epigallocatechin and epicatechin gallate. The results indicated that pecan nut shell extracts are effective against tumor cells growth and may be considered as an alternative to the treatment of cancer. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/28807853/Aqueous_extract_from_pecan_nut_[Carya_illinoinensis__Wangenh__C__Koch]_shell_show_activity_against_breast_cancer_cell_line_MCF_7_and_Ehrlich_ascites_tumor_in_Balb_C_mice_ DB - PRIME DP - Unbound Medicine ER -