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Diallyl trisulfide (DATS) suppresses benzene-induced cytopenia by modulating haematopoietic cell apoptosis.
Environ Pollut. 2017 Dec; 231(Pt 1):301-310.EP

Abstract

Benzene is a well-known occupational and environmental toxicant associated with cytopenia, which is characterized by a disorder in the peripheral blood cell counts. However, no effective preventive strategy has been developed yet to tackle the exposure to benzene in daily life. The aim of this study was to evaluate the protective effects of diallyl trisulfide (DATS) on benzene-induced haematopoietic damage and to reveal its potential mechanisms of action. In our study, male Sprague-Dawley rats were divided into six groups. Rats were administered with benzene (1.3 g/kg BW by gavage) to establish the benzene poisoning model, while the DATS treatment groups were treated with benzene plus DATS (15 mg/kg, 30 mg/kg, 45 mg/kg, respectively, by gavage) for 28 days. Our results demonstrated that the counts of peripheral blood WBC and RBC decreased to 31.0% and 79.2%, respectively, in the benzene poisoning model group compared to the control. However, blood cell counts were restored by DATS treatment (30 mg/kg, 45 mg/kg). The apoptosis rates of peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BMCs) were increased to 274% and 284%, respectively, following benzene exposure. Furthermore, expression levels of Bcl-2, PI3K and p-Akt were downregulated and those of Bax were upregulated in both cell types. Moreover, the oxidative parameters (oxygen species, malonaldehyde) were significantly increased, while the non-enzymatic GSH/GSSG ratios and the activities of enzymatic antioxidants (superoxide dismutase, glutathione peroxidase and catalase) were decreased. Interestingly, DATS treatment can restore the WBC number by 267.1% and 304.8% while RBC number by 108.6% and 117.7% in 30,45 mg/k DATS treated groups. In summary, we demonstrated that benzene-induced cytopenia was related to the apoptosis of PBMCs and BMCs, and DATS treatment could prevent benzene-induced cytopenia by suppressing oxidative stress-mediated cell apoptosis via the PI3K/Akt pathway.

Authors+Show Affiliations

Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, China.Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, China.Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, China.Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, China.Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, China.Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, China.Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong 250012, China. Electronic address: keqinx@sdu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28810199

Citation

Han, Wenting, et al. "Diallyl Trisulfide (DATS) Suppresses Benzene-induced Cytopenia By Modulating Haematopoietic Cell Apoptosis." Environmental Pollution (Barking, Essex : 1987), vol. 231, no. Pt 1, 2017, pp. 301-310.
Han W, Wang S, Jiang L, et al. Diallyl trisulfide (DATS) suppresses benzene-induced cytopenia by modulating haematopoietic cell apoptosis. Environ Pollut. 2017;231(Pt 1):301-310.
Han, W., Wang, S., Jiang, L., Wang, H., Li, M., Wang, X., & Xie, K. (2017). Diallyl trisulfide (DATS) suppresses benzene-induced cytopenia by modulating haematopoietic cell apoptosis. Environmental Pollution (Barking, Essex : 1987), 231(Pt 1), 301-310. https://doi.org/10.1016/j.envpol.2017.07.069
Han W, et al. Diallyl Trisulfide (DATS) Suppresses Benzene-induced Cytopenia By Modulating Haematopoietic Cell Apoptosis. Environ Pollut. 2017;231(Pt 1):301-310. PubMed PMID: 28810199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diallyl trisulfide (DATS) suppresses benzene-induced cytopenia by modulating haematopoietic cell apoptosis. AU - Han,Wenting, AU - Wang,Shuo, AU - Jiang,Lulu, AU - Wang,Hui, AU - Li,Ming, AU - Wang,Xujing, AU - Xie,Keqin, Y1 - 2017/08/12/ PY - 2016/08/31/received PY - 2017/07/19/revised PY - 2017/07/19/accepted PY - 2017/8/16/pubmed PY - 2018/2/17/medline PY - 2017/8/16/entrez KW - Benzene poisoning KW - Cytopenia KW - Diallyl trisulfide KW - Oxidative stress SP - 301 EP - 310 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ. Pollut. VL - 231 IS - Pt 1 N2 - Benzene is a well-known occupational and environmental toxicant associated with cytopenia, which is characterized by a disorder in the peripheral blood cell counts. However, no effective preventive strategy has been developed yet to tackle the exposure to benzene in daily life. The aim of this study was to evaluate the protective effects of diallyl trisulfide (DATS) on benzene-induced haematopoietic damage and to reveal its potential mechanisms of action. In our study, male Sprague-Dawley rats were divided into six groups. Rats were administered with benzene (1.3 g/kg BW by gavage) to establish the benzene poisoning model, while the DATS treatment groups were treated with benzene plus DATS (15 mg/kg, 30 mg/kg, 45 mg/kg, respectively, by gavage) for 28 days. Our results demonstrated that the counts of peripheral blood WBC and RBC decreased to 31.0% and 79.2%, respectively, in the benzene poisoning model group compared to the control. However, blood cell counts were restored by DATS treatment (30 mg/kg, 45 mg/kg). The apoptosis rates of peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BMCs) were increased to 274% and 284%, respectively, following benzene exposure. Furthermore, expression levels of Bcl-2, PI3K and p-Akt were downregulated and those of Bax were upregulated in both cell types. Moreover, the oxidative parameters (oxygen species, malonaldehyde) were significantly increased, while the non-enzymatic GSH/GSSG ratios and the activities of enzymatic antioxidants (superoxide dismutase, glutathione peroxidase and catalase) were decreased. Interestingly, DATS treatment can restore the WBC number by 267.1% and 304.8% while RBC number by 108.6% and 117.7% in 30,45 mg/k DATS treated groups. In summary, we demonstrated that benzene-induced cytopenia was related to the apoptosis of PBMCs and BMCs, and DATS treatment could prevent benzene-induced cytopenia by suppressing oxidative stress-mediated cell apoptosis via the PI3K/Akt pathway. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/28810199/Diallyl_trisulfide__DATS__suppresses_benzene_induced_cytopenia_by_modulating_haematopoietic_cell_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(16)31092-2 DB - PRIME DP - Unbound Medicine ER -