Tags

Type your tag names separated by a space and hit enter

Valuable antibody detection method for classifying hepatitis E virus genotypes.
J Med Virol. 2018 01; 90(1):142-147.JM

Abstract

Nucleotide-based methods are conventionally used to classify the hepatitis E virus (HEV) genotypes. A serological enzyme immunoassay (EIA) using open reading frame 3 (ORF3) C-terminal peptides was developed to conveniently and accurately classify and evaluate the genotypes of HEV. The sera of mice immunized with HEV genotype 1, 3, and 4 reacted highly specifically to the peptides of the corresponding genotypes. Most (84.2%) clinical sera infected with HEV genotype 4 were positive for anti-HEV antibodies when tested with the ORF3 peptides of genotype 4, but were negative for genotypes 1 and 3. Monkey and clinical serial sera infected with HEV reacted strongly to the homologous genotype ORF3 peptides. The indirect EIAs were more sensitive, with stronger reactivity, than commercial anti-HEV immunoglobulin G assays when serial sera from monkeys infected with HEV genotype 1 or 4 were tested. All our results indicate that the serological typing EIA assays described in this study are more effective and convenient for the classification of HEV genotypes than molecular approaches, and can be used to screen large numbers of serum samples and differentiate genotypes for the diagnosis of HEV infections.

Authors+Show Affiliations

Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control, Tiantanxili, Beijing, China.Health Science Center, Hebei University, Yuhuadonglu, Baoding, China.Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control, Tiantanxili, Beijing, China.Henan Provincial Center for Disease Prevention and Control, Nongyenanlu, Zhengzhou, China.Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control, Tiantanxili, Beijing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28817198

Citation

Zhao, Chenyan, et al. "Valuable Antibody Detection Method for Classifying Hepatitis E Virus Genotypes." Journal of Medical Virology, vol. 90, no. 1, 2018, pp. 142-147.
Zhao C, Geng Y, Huang W, et al. Valuable antibody detection method for classifying hepatitis E virus genotypes. J Med Virol. 2018;90(1):142-147.
Zhao, C., Geng, Y., Huang, W., Ma, H., & Wang, Y. (2018). Valuable antibody detection method for classifying hepatitis E virus genotypes. Journal of Medical Virology, 90(1), 142-147. https://doi.org/10.1002/jmv.24915
Zhao C, et al. Valuable Antibody Detection Method for Classifying Hepatitis E Virus Genotypes. J Med Virol. 2018;90(1):142-147. PubMed PMID: 28817198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Valuable antibody detection method for classifying hepatitis E virus genotypes. AU - Zhao,Chenyan, AU - Geng,Yansheng, AU - Huang,Weijing, AU - Ma,Hongxia, AU - Wang,Youchun, Y1 - 2017/09/04/ PY - 2017/03/15/received PY - 2017/07/31/accepted PY - 2017/8/18/pubmed PY - 2018/7/12/medline PY - 2017/8/18/entrez KW - HEV genotypes KW - ORF3 peptides KW - anti-HEV immunoglobulin G KW - enzyme immunoassay KW - hepatitis E virus SP - 142 EP - 147 JF - Journal of medical virology JO - J Med Virol VL - 90 IS - 1 N2 - Nucleotide-based methods are conventionally used to classify the hepatitis E virus (HEV) genotypes. A serological enzyme immunoassay (EIA) using open reading frame 3 (ORF3) C-terminal peptides was developed to conveniently and accurately classify and evaluate the genotypes of HEV. The sera of mice immunized with HEV genotype 1, 3, and 4 reacted highly specifically to the peptides of the corresponding genotypes. Most (84.2%) clinical sera infected with HEV genotype 4 were positive for anti-HEV antibodies when tested with the ORF3 peptides of genotype 4, but were negative for genotypes 1 and 3. Monkey and clinical serial sera infected with HEV reacted strongly to the homologous genotype ORF3 peptides. The indirect EIAs were more sensitive, with stronger reactivity, than commercial anti-HEV immunoglobulin G assays when serial sera from monkeys infected with HEV genotype 1 or 4 were tested. All our results indicate that the serological typing EIA assays described in this study are more effective and convenient for the classification of HEV genotypes than molecular approaches, and can be used to screen large numbers of serum samples and differentiate genotypes for the diagnosis of HEV infections. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/28817198/Valuable_antibody_detection_method_for_classifying_hepatitis_E_virus_genotypes_ L2 - https://doi.org/10.1002/jmv.24915 DB - PRIME DP - Unbound Medicine ER -