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Hepatoprotective and antioxidant effects of single clove garlic against CCl4-induced hepatic damage in rabbits.
BMC Complement Altern Med. 2017 Aug 17; 17(1):411.BC

Abstract

BACKGROUND

The increase in demand and consumption of single clove garlic or 'Solo garlic' (Allium sativum) has resulted in an increase in research on its therapeutic properties. The present study aims to evaluate the antioxidant activities, oxidant-scavenging efficiency and preventive effects of SCG (single clove garlic) and MCG (multi clove garlic) on CCl4-induced acute hepatotoxicity in male rabbits.

METHODS

For this purpose, rabbits were orally administered with 3 ml of CCl4 /kg of body weight, followed by 0.8 g of MCG or SCG/kg twice a week for three successive weeks. Oxidative hepatotoxicity was then assessed.

RESULTS

SCG extracts exhibited higher antioxidant capacity than the MCG extract. Scavenging ability of SCG showed significant (p < 0.05) elevation against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals in comparison to MCG. In addition, total phenolic content of SCG was significantly elevated (p < 0.001), thereby suggesting that the composition of garlic storage constituents varies with the number of cloves present. CCl4-induced hepatotoxicity demonstrated histological changes including severe damage in the structure of liver tissues which correlated well to oxidative stress levels. Simultaneously, administration of SCG resulted in a significant reduction of serum alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TB) levels in addition to improvement in some histological parameters. Low levels of lipid peroxidation (malondialdehyde, MDA) (p < 0.001), along with a huge reduction in peroxidase (POx) (p < 0.001) revealed protection against oxidative toxicity in the liver homogenate. Higher levels of catalase (CAT) (p < 0.001) and superoxide dismutase (SOD) (p < 0.05) when compared to the MCG test (TM) group indicates that removal of H2O2 is based on CAT activity in SCG test (TS) group rather than the POx activity demonstrated in the former group.

CONCLUSION

The present study indicates that SCG possesses more protective ability than MCG against CCl4-induced liver injury and might be an effective alternative medicine against acute oxidative liver toxicity.

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Authors+Show Affiliations

Naji KM
Biochemistry section, Department of Chemistry, Faculty of science, Sana'a University, P.O. Box 13499, Sana'a, Yemen. khalid.m.naji@gmail.com.
Al-Shaibani ES
Zoology section, Department of Biology, Faculty of Science, Sana'a University, Sana'a, Yemen.
Alhadi FA
Botany section, Department of Biology, Faculty of Science, Sana'a University, Sana'a, Yemen.
Al-Soudi SA
Zoology section, Department of Biology, Faculty of Science, Sana'a University, Sana'a, Yemen.
D'souza MR
Department of Biochemistry, Mount Carmel College, No.58, Palace Road, Vasanth Nagar, Bengaluru, Karnataka, 560052, India.

MeSH

Alanine TransaminaseAnimalsAntioxidantsAspartate AminotransferasesBilirubinCarbon TetrachlorideCatalaseChemical and Drug Induced Liver InjuryGarlicLipid PeroxidationLiverMaleMalondialdehydeOxidative StressPeroxidasePhenolsPhytotherapyPlant ExtractsRabbitsSuperoxide DismutaseSuperoxides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28818066

Citation

Naji, Khalid Mohammed, et al. "Hepatoprotective and Antioxidant Effects of Single Clove Garlic Against CCl4-induced Hepatic Damage in Rabbits." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 411.
Naji KM, Al-Shaibani ES, Alhadi FA, et al. Hepatoprotective and antioxidant effects of single clove garlic against CCl4-induced hepatic damage in rabbits. BMC Complement Altern Med. 2017;17(1):411.
Naji, K. M., Al-Shaibani, E. S., Alhadi, F. A., Al-Soudi, S. A., & D'souza, M. R. (2017). Hepatoprotective and antioxidant effects of single clove garlic against CCl4-induced hepatic damage in rabbits. BMC Complementary and Alternative Medicine, 17(1), 411. https://doi.org/10.1186/s12906-017-1916-8
Naji KM, et al. Hepatoprotective and Antioxidant Effects of Single Clove Garlic Against CCl4-induced Hepatic Damage in Rabbits. BMC Complement Altern Med. 2017 Aug 17;17(1):411. PubMed PMID: 28818066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective and antioxidant effects of single clove garlic against CCl4-induced hepatic damage in rabbits. AU - Naji,Khalid Mohammed, AU - Al-Shaibani,Elham Shukri, AU - Alhadi,Fatima A, AU - Al-Soudi,Safa'a Abdulrzaq, AU - D'souza,Myrene R, Y1 - 2017/08/17/ PY - 2017/03/21/received PY - 2017/08/08/accepted PY - 2017/8/19/entrez PY - 2017/8/19/pubmed PY - 2017/9/2/medline KW - Allium sativum KW - Antioxidant KW - Biochemical parameters KW - Carbon tetrachloride KW - Hepatotoxicity KW - Oxidative toxicity KW - Single clove garlic SP - 411 EP - 411 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: The increase in demand and consumption of single clove garlic or 'Solo garlic' (Allium sativum) has resulted in an increase in research on its therapeutic properties. The present study aims to evaluate the antioxidant activities, oxidant-scavenging efficiency and preventive effects of SCG (single clove garlic) and MCG (multi clove garlic) on CCl4-induced acute hepatotoxicity in male rabbits. METHODS: For this purpose, rabbits were orally administered with 3 ml of CCl4 /kg of body weight, followed by 0.8 g of MCG or SCG/kg twice a week for three successive weeks. Oxidative hepatotoxicity was then assessed. RESULTS: SCG extracts exhibited higher antioxidant capacity than the MCG extract. Scavenging ability of SCG showed significant (p < 0.05) elevation against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals in comparison to MCG. In addition, total phenolic content of SCG was significantly elevated (p < 0.001), thereby suggesting that the composition of garlic storage constituents varies with the number of cloves present. CCl4-induced hepatotoxicity demonstrated histological changes including severe damage in the structure of liver tissues which correlated well to oxidative stress levels. Simultaneously, administration of SCG resulted in a significant reduction of serum alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TB) levels in addition to improvement in some histological parameters. Low levels of lipid peroxidation (malondialdehyde, MDA) (p < 0.001), along with a huge reduction in peroxidase (POx) (p < 0.001) revealed protection against oxidative toxicity in the liver homogenate. Higher levels of catalase (CAT) (p < 0.001) and superoxide dismutase (SOD) (p < 0.05) when compared to the MCG test (TM) group indicates that removal of H2O2 is based on CAT activity in SCG test (TS) group rather than the POx activity demonstrated in the former group. CONCLUSION: The present study indicates that SCG possesses more protective ability than MCG against CCl4-induced liver injury and might be an effective alternative medicine against acute oxidative liver toxicity. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28818066/Hepatoprotective_and_antioxidant_effects_of_single_clove_garlic_against_CCl4_induced_hepatic_damage_in_rabbits_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1916-8 DB - PRIME DP - Unbound Medicine ER -