Abstract
Auxin is a main plant growth hormone crucial in a multitude of developmental processes in plants. Auxin biosynthesis via the tryptophan aminotransferase of arabidopsis (TAA)/YUCCA (YUC) route involving tryptophan aminotransferases and YUC flavin-dependent monooxygenases that produce the auxin indole-3-acetic acid (IAA) from tryptophan is currently the most researched auxin biosynthetic pathway. Previous data showed that, in maize and arabidopsis, TAA/YUC-dependent auxin biosynthesis can be detected in endoplasmic reticulum (ER) microsomal fractions, and a subset of auxin biosynthetic proteins are localized to the ER, mainly due to transmembrane domains (TMD). The phylogeny presented here for TAA/TAR (tryptophan aminotransferase related) and YUC proteins analyses phylogenetic groups as well as transmembrane domains for ER-membrane localisation. In addition, RNAseq datasets are analysed for transcript abundance of YUC and TAA/TAR proteins in Arabidopsis thaliana. We show that ER membrane localisation for TAA/YUC proteins involved in auxin biosynthesis is already present early on in the evolution of mosses and club mosses. ER membrane anchored YUC proteins can mainly be found in roots, while cytosolic proteins are more abundant in the shoot. The distribution between the different phylogenetic classes in root and shoot may well originate from gene duplications, and the phylogenetic groups detected also overlap with the biological function.
TY - JOUR
T1 - Bioinformatics Analysis of Phylogeny and Transcription of TAA/YUC Auxin Biosynthetic Genes.
AU - Poulet,Axel,
AU - Kriechbaumer,Verena,
Y1 - 2017/08/18/
PY - 2017/06/20/received
PY - 2017/07/19/revised
PY - 2017/08/13/accepted
PY - 2017/8/19/entrez
PY - 2017/8/19/pubmed
PY - 2018/5/1/medline
KW - RNAseq
KW - YUCCA (YUC)
KW - auxin
KW - endoplasmic reticulum (ER)
KW - gene duplication
KW - indole-3-acetic acid (IAA)
KW - phylogeny
KW - transmembrane domain (TMD)
KW - tryptophan aminotransferase of arabidopsis (TAA)
KW - tryptophan aminotransferase related (TAR)
JF - International journal of molecular sciences
JO - Int J Mol Sci
VL - 18
IS - 8
N2 - Auxin is a main plant growth hormone crucial in a multitude of developmental processes in plants. Auxin biosynthesis via the tryptophan aminotransferase of arabidopsis (TAA)/YUCCA (YUC) route involving tryptophan aminotransferases and YUC flavin-dependent monooxygenases that produce the auxin indole-3-acetic acid (IAA) from tryptophan is currently the most researched auxin biosynthetic pathway. Previous data showed that, in maize and arabidopsis, TAA/YUC-dependent auxin biosynthesis can be detected in endoplasmic reticulum (ER) microsomal fractions, and a subset of auxin biosynthetic proteins are localized to the ER, mainly due to transmembrane domains (TMD). The phylogeny presented here for TAA/TAR (tryptophan aminotransferase related) and YUC proteins analyses phylogenetic groups as well as transmembrane domains for ER-membrane localisation. In addition, RNAseq datasets are analysed for transcript abundance of YUC and TAA/TAR proteins in Arabidopsis thaliana. We show that ER membrane localisation for TAA/YUC proteins involved in auxin biosynthesis is already present early on in the evolution of mosses and club mosses. ER membrane anchored YUC proteins can mainly be found in roots, while cytosolic proteins are more abundant in the shoot. The distribution between the different phylogenetic classes in root and shoot may well originate from gene duplications, and the phylogenetic groups detected also overlap with the biological function.
SN - 1422-0067
UR - https://www.unboundmedicine.com/medline/citation/28820425/Bioinformatics_Analysis_of_Phylogeny_and_Transcription_of_TAA/YUC_Auxin_Biosynthetic_Genes_
L2 - https://www.mdpi.com/resolver?pii=ijms18081791
DB - PRIME
DP - Unbound Medicine
ER -
