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The whole genomic analysis of orf virus strain HN3/12 isolated from Henan province, central China.
BMC Vet Res 2017; 13(1):260BV

Abstract

BACKGROUND

The Orf virus (ORFV) is the causative agent of orf, a globally-occurring, acute, pustular, contagious disease affecting sheep, goats and humans with a worldwide distribution. Currently, the genomic analysis of four ORFV strains from the Fujian province in southern China and a NA1/11 strain isolated from the Jilin province in northeast China have been reported. However, little is known about the genomic information of ORFV strains from central China.

RESULTS

From a recent outbreak in a sheep herd in the Henan province of central China, a novel ORFV strain (HN3/12) was isolated and cultured in ovine fetal turbinate (OFTu) cells. The strain was identified as HN3/12 and verified by PCR based on the DNA sequences of 011 and 059 genes. The whole genomic sequence of this isolate was determined by Next Generation Sequencing technology. To determine the genetic characteristics of the HN3/12 strain, phylogenetic analysis of the 011 and 059 genes and amino acid sequence alignment of the HN3/12 strain were performed and compared with reference parapoxvirus strains.

CONCLUSIONS

The HN3/12 genome is 136,643 bp in length, contains 63.67% G + C and encodes 132 putative genes. Phylogenetic analysis of the 011 and 059 nucleotide sequences showed that this viral strain was similar to the NA1/11 isolate. The homology analysis indicates that HN3/12 has 93% to 98% identity with published ORFV strains at amino acid level. When open reading frames (ORFs) were aligned among the HN3/12 and four Fujian ORFV strains, most of them have identities greater than 90% and only a few less than 60%. The availability of the whole genomic sequence of HN3/12 aids in our understanding of, and provides new insights into, the genetic diversity of ORFV.

Authors+Show Affiliations

Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China.Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China.Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China.Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China.Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China.Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China.Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China. sluo815@gmail.com. Department of Laboratory Medicine, School of Stomatology and Medicine, Foshan University, 5 Hebin Road, Foshan, Guangdong Province, 528000, People's Republic of China. sluo815@gmail.com.Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, People's Republic of China. haowa@126.com. Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, People's Republic of China. haowa@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28821255

Citation

Chen, Huiqin, et al. "The Whole Genomic Analysis of Orf Virus Strain HN3/12 Isolated From Henan Province, Central China." BMC Veterinary Research, vol. 13, no. 1, 2017, p. 260.
Chen H, Li W, Kuang Z, et al. The whole genomic analysis of orf virus strain HN3/12 isolated from Henan province, central China. BMC Vet Res. 2017;13(1):260.
Chen, H., Li, W., Kuang, Z., Chen, D., Liao, X., Li, M., ... Hao, W. (2017). The whole genomic analysis of orf virus strain HN3/12 isolated from Henan province, central China. BMC Veterinary Research, 13(1), p. 260. doi:10.1186/s12917-017-1178-1.
Chen H, et al. The Whole Genomic Analysis of Orf Virus Strain HN3/12 Isolated From Henan Province, Central China. BMC Vet Res. 2017 Aug 18;13(1):260. PubMed PMID: 28821255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The whole genomic analysis of orf virus strain HN3/12 isolated from Henan province, central China. AU - Chen,Huiqin, AU - Li,Wei, AU - Kuang,Zhenzhan, AU - Chen,Daxiang, AU - Liao,Xiaoqing, AU - Li,Ming, AU - Luo,Shuhong, AU - Hao,Wenbo, Y1 - 2017/08/18/ PY - 2016/12/21/received PY - 2017/08/10/accepted PY - 2017/8/20/entrez PY - 2017/8/20/pubmed PY - 2018/3/15/medline KW - Genomic sequencing KW - HN3/12 KW - ORFV KW - Parapoxvirus KW - Phylogenetic analysis SP - 260 EP - 260 JF - BMC veterinary research JO - BMC Vet. Res. VL - 13 IS - 1 N2 - BACKGROUND: The Orf virus (ORFV) is the causative agent of orf, a globally-occurring, acute, pustular, contagious disease affecting sheep, goats and humans with a worldwide distribution. Currently, the genomic analysis of four ORFV strains from the Fujian province in southern China and a NA1/11 strain isolated from the Jilin province in northeast China have been reported. However, little is known about the genomic information of ORFV strains from central China. RESULTS: From a recent outbreak in a sheep herd in the Henan province of central China, a novel ORFV strain (HN3/12) was isolated and cultured in ovine fetal turbinate (OFTu) cells. The strain was identified as HN3/12 and verified by PCR based on the DNA sequences of 011 and 059 genes. The whole genomic sequence of this isolate was determined by Next Generation Sequencing technology. To determine the genetic characteristics of the HN3/12 strain, phylogenetic analysis of the 011 and 059 genes and amino acid sequence alignment of the HN3/12 strain were performed and compared with reference parapoxvirus strains. CONCLUSIONS: The HN3/12 genome is 136,643 bp in length, contains 63.67% G + C and encodes 132 putative genes. Phylogenetic analysis of the 011 and 059 nucleotide sequences showed that this viral strain was similar to the NA1/11 isolate. The homology analysis indicates that HN3/12 has 93% to 98% identity with published ORFV strains at amino acid level. When open reading frames (ORFs) were aligned among the HN3/12 and four Fujian ORFV strains, most of them have identities greater than 90% and only a few less than 60%. The availability of the whole genomic sequence of HN3/12 aids in our understanding of, and provides new insights into, the genetic diversity of ORFV. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/28821255/The_whole_genomic_analysis_of_orf_virus_strain_HN3/12_isolated_from_Henan_province_central_China_ L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1178-1 DB - PRIME DP - Unbound Medicine ER -