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Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor.
J Food Sci. 2017 Oct; 82(10):2357-2363.JF

Abstract

Detection of foodborne pathogens at very low levels is still a challenge. A custom-built multichannel surface plasmon resonance (SPR) biosensor and simultaneous enrichment broth (SEB) were used to develop a simultaneous detection method for 3 important foodborne pathogens, Escherichia coli O157:H7 (O157:H7), Salmonella enteritidis, and Listeria monocytogenes, at a very low level. These 3 foodborne pathogens at a very low level (14, 6, and 28 CFU/25 g (mL) for O157:H7, S. enteritidis, and L. monocytogenes, respectively) were inoculated in SEB and incubated at 37 ˚C for 24 h. Sample prepared from the simultaneous enrichment culture was analyzed using the multichannel SPR biosensor and sensor chip immobilized with polyclonal antibodies specific to each of the target pathogens. O157:H7, S. enteritidis, and L. monocytogenes in chicken were detected simultaneously at an inoculum dose of 14, 6, and 28 CFU/25 g, respectively. Our method using a custom-built multichannel SPR biosensor and enrichment in SEB is expected as a rapid and simultaneous detection method for low levels of O157:H7, S. enteritidis, and L. monocytogenes in food.

PRACTICAL APPLICATION

Our method is expected as a rapid and simultaneous detection method for pathogens at very low levels. It has great potential for safety control of food and microbiological detection applications.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang X
College of Food Science and Engineering, Jilin Univ., No.5988 Renmin St., Nanguan District, Changchun 130022, P.R.China. Dept. of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi 812-8581, Japan.
Tsuji S
Dept. of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi 812-8581, Japan.
Kitaoka H
Dept. of Food and Health Science, Intl. College of Arts and Science, Fukuoka Women's Univ, 1-1-1 Kasumigaoka, Higashi-ku, Fukuoka-shi 813-8529, Japan.
Kobayashi H
Dept. of Food and Health Science, Intl. College of Arts and Science, Fukuoka Women's Univ, 1-1-1 Kasumigaoka, Higashi-ku, Fukuoka-shi 813-8529, Japan.
Tamai M
Dept. of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi 812-8581, Japan.
Honjoh KI
Dept. of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi 812-8581, Japan.
Miyamoto T
Dept. of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu Univ., 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi 812-8581, Japan.

MeSH

AnimalsBiosensing TechniquesChickensColony Count, MicrobialEscherichia coli O157Food ContaminationFood MicrobiologyListeria monocytogenesMeatSalmonella enteritidisSurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28833106

Citation

Zhang, Xiaoguang, et al. "Simultaneous Detection of Escherichia Coli O157:H7, Salmonella Enteritidis, and Listeria Monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor." Journal of Food Science, vol. 82, no. 10, 2017, pp. 2357-2363.
Zhang X, Tsuji S, Kitaoka H, et al. Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor. J Food Sci. 2017;82(10):2357-2363.
Zhang, X., Tsuji, S., Kitaoka, H., Kobayashi, H., Tamai, M., Honjoh, K. I., & Miyamoto, T. (2017). Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor. Journal of Food Science, 82(10), 2357-2363. https://doi.org/10.1111/1750-3841.13843
Zhang X, et al. Simultaneous Detection of Escherichia Coli O157:H7, Salmonella Enteritidis, and Listeria Monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor. J Food Sci. 2017;82(10):2357-2363. PubMed PMID: 28833106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor. AU - Zhang,Xiaoguang, AU - Tsuji,Sachiko, AU - Kitaoka,Hayato, AU - Kobayashi,Hiroshi, AU - Tamai,Mitsuru, AU - Honjoh,Ken-Ichi, AU - Miyamoto,Takahisa, Y1 - 2017/08/23/ PY - 2017/02/09/received PY - 2017/07/08/revised PY - 2017/07/16/accepted PY - 2017/8/24/pubmed PY - 2018/3/7/medline PY - 2017/8/24/entrez KW - antibody KW - biosensor KW - detection KW - foodborne pathogens KW - surface plasmon resonance SP - 2357 EP - 2363 JF - Journal of food science JO - J Food Sci VL - 82 IS - 10 N2 - : Detection of foodborne pathogens at very low levels is still a challenge. A custom-built multichannel surface plasmon resonance (SPR) biosensor and simultaneous enrichment broth (SEB) were used to develop a simultaneous detection method for 3 important foodborne pathogens, Escherichia coli O157:H7 (O157:H7), Salmonella enteritidis, and Listeria monocytogenes, at a very low level. These 3 foodborne pathogens at a very low level (14, 6, and 28 CFU/25 g (mL) for O157:H7, S. enteritidis, and L. monocytogenes, respectively) were inoculated in SEB and incubated at 37 ˚C for 24 h. Sample prepared from the simultaneous enrichment culture was analyzed using the multichannel SPR biosensor and sensor chip immobilized with polyclonal antibodies specific to each of the target pathogens. O157:H7, S. enteritidis, and L. monocytogenes in chicken were detected simultaneously at an inoculum dose of 14, 6, and 28 CFU/25 g, respectively. Our method using a custom-built multichannel SPR biosensor and enrichment in SEB is expected as a rapid and simultaneous detection method for low levels of O157:H7, S. enteritidis, and L. monocytogenes in food. PRACTICAL APPLICATION: Our method is expected as a rapid and simultaneous detection method for pathogens at very low levels. It has great potential for safety control of food and microbiological detection applications. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/28833106/Simultaneous_Detection_of_Escherichia_coli_O157:H7_Salmonella_enteritidis_and_Listeria_monocytogenes_at_a_Very_Low_Level_Using_Simultaneous_Enrichment_Broth_and_Multichannel_SPR_Biosensor_ L2 - https://doi.org/10.1111/1750-3841.13843 DB - PRIME DP - Unbound Medicine ER -
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