Citation
Xu, Wen, et al. "Simultaneous Determination of Morphine-6-d-glucuronide, Morphine-3-d-glucuronide and Morphine in Human Plasma and Urine By Ultra-performance Liquid Chromatography-tandem Mass Spectrometry: Application to M6G Injection Pharmacokinetic Study." Biomedical Chromatography : BMC, vol. 32, no. 2, 2018.
Xu W, Zhuang Q, Chen X, et al. Simultaneous determination of morphine-6-d-glucuronide, morphine-3-d-glucuronide and morphine in human plasma and urine by ultra-performance liquid chromatography-tandem mass spectrometry: Application to M6G injection pharmacokinetic study. Biomed Chromatogr. 2018;32(2).
Xu, W., Zhuang, Q., Chen, X., Jiang, J., Hu, P., & Wang, H. (2018). Simultaneous determination of morphine-6-d-glucuronide, morphine-3-d-glucuronide and morphine in human plasma and urine by ultra-performance liquid chromatography-tandem mass spectrometry: Application to M6G injection pharmacokinetic study. Biomedical Chromatography : BMC, 32(2). https://doi.org/10.1002/bmc.4066
Xu W, et al. Simultaneous Determination of Morphine-6-d-glucuronide, Morphine-3-d-glucuronide and Morphine in Human Plasma and Urine By Ultra-performance Liquid Chromatography-tandem Mass Spectrometry: Application to M6G Injection Pharmacokinetic Study. Biomed Chromatogr. 2018;32(2) PubMed PMID: 28833311.
TY - JOUR
T1 - Simultaneous determination of morphine-6-d-glucuronide, morphine-3-d-glucuronide and morphine in human plasma and urine by ultra-performance liquid chromatography-tandem mass spectrometry: Application to M6G injection pharmacokinetic study.
AU - Xu,Wen,
AU - Zhuang,Quankun,
AU - Chen,Xia,
AU - Jiang,Ji,
AU - Hu,Pei,
AU - Wang,Hongyun,
Y1 - 2017/09/15/
PY - 2017/05/19/received
PY - 2017/07/25/revised
PY - 2017/08/14/accepted
PY - 2017/8/24/pubmed
PY - 2018/2/24/medline
PY - 2017/8/24/entrez
KW - UPLC-MS/MS
KW - morphine
KW - morphine-3-d-glucuronide
KW - morphine-6-d-glucuronide
JF - Biomedical chromatography : BMC
JO - Biomed Chromatogr
VL - 32
IS - 2
N2 - A robust ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of morphine-6-d-glucuronide (M6G), morphine-3-d-glucuronide (M3G) and morphine (MOR) in human plasma and urine has been developed and validated. The analytes of interest were extracted from plasma by protein precipitation. The urine sample was prepared by dilution. Both plasma and urine samples were chromatographed on an Acquity UPLC HSS T3 column using gradient elution. Detection was performed on a Xevo TQ-S tandem mass spectrometer in multiple reaction monitoring mode using positive electrospray ionization. Matrix interferences were not observed at the retention time of the analytes and internal standard, naloxone-D5. The lower limits of quantitation of plasma and urine were 2/0.5/0.5 and 20/4/2 ng/mL for M6G/M3G/MOR, respectively. Calibration curves were linear over the concentration ranges of 2-2000/0.5-500/0.5-500 and 20-20,000/4-4000/2-2000 ng/mL for M6G/M3G/MOR in plasma and urine samples, respectively. The precision was <7.14% and the accuracy was within 85-115%. Furthermore, stability of the analytes at various conditions, dilution integrity, extraction recovery and matrix effect were assessed. Finally, this quantitative method was successfully applied to the pharmacokinetic study of M6G injection in Chinese noncancer pain patients.
SN - 1099-0801
UR - https://www.unboundmedicine.com/medline/citation/28833311/Simultaneous_determination_of_morphine_6_d_glucuronide_morphine_3_d_glucuronide_and_morphine_in_human_plasma_and_urine_by_ultra_performance_liquid_chromatography_tandem_mass_spectrometry:_Application_to_M6G_injection_pharmacokinetic_study_
L2 - https://doi.org/10.1002/bmc.4066
DB - PRIME
DP - Unbound Medicine
ER -
