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Potency and Cytotoxicity of a Novel Gallium-Containing Mesoporous Bioactive Glass/Chitosan Composite Scaffold as Hemostatic Agents.
ACS Appl Mater Interfaces 2017; 9(37):31381-31392AA

Abstract

Chitosan-based hemostats are promising candidates for immediate hemorrhage control. However, they have some disadvantages and require further improvement to achieve the desired hemostatic efficiency. Here, a series of 1% Ga2O3-containing mesoporous bioactive glass-chitosan composite scaffolds (Ga-MBG/CHT) were constructed by the lyophilization process and the effect of various concentrations of Ga-MBG (10, 30, and 50 wt %) on the hemostatic function of the CHT scaffold was assessed as compared to that of Celox Rapid gauze (CXR), a current commercially available chitosan-coated hemostatic gauze. The prepared scaffolds exhibited >79% porosity and showed increased water uptake compared to that in CXR. The results of coagulation studies showed that pure CHT and composite scaffolds exhibited increased hemostatic performance with respect to CXR. Furthermore, the composite scaffold with the highest Ga-MBG content (50 wt %) had increased capability to enhancing thrombus generation, blood clotting, and platelet adhesion and aggregation than that of the scaffold made of pure CHT. The antibacterial efficacy and biocompatibility of the prepared scaffolds were also assessed by a time-killing assay and an Alamar Blue assay, respectively. Our results show that the antibacterial effect of 50% Ga-MBG/CHT was more pronounced than that of CHT and CXR. The cell viability results also demonstrated that Ga-MBG/CHT composite scaffolds had good biocompatibility, which facilitates the spreading and proliferation of human dermal fibroblast cells even with 50 wt % Ga-MBG loading. These results suggest that Ga-MBG/CHT scaffolds could be a promising hemostatic candidate for improving hemostasis in critical situations.

Authors+Show Affiliations

No affiliation info availableSchool of Engineering, Monash University , 47500 Bandar Sunway, Selangor, Malaysia.No affiliation info availableACLabs - Laboratori di Chimica Applicata, Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università Federico II , P.le Tecchio 80, 80125 Napoli, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableDepartment of Mechanical & Industrial Engineering, Ryerson University , Toronto M5B 2K3, Ontario, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28836753

Citation

Pourshahrestani, Sara, et al. "Potency and Cytotoxicity of a Novel Gallium-Containing Mesoporous Bioactive Glass/Chitosan Composite Scaffold as Hemostatic Agents." ACS Applied Materials & Interfaces, vol. 9, no. 37, 2017, pp. 31381-31392.
Pourshahrestani S, Zeimaran E, Kadri NA, et al. Potency and Cytotoxicity of a Novel Gallium-Containing Mesoporous Bioactive Glass/Chitosan Composite Scaffold as Hemostatic Agents. ACS Appl Mater Interfaces. 2017;9(37):31381-31392.
Pourshahrestani, S., Zeimaran, E., Kadri, N. A., Gargiulo, N., Jindal, H. M., Naveen, S. V., ... Towler, M. R. (2017). Potency and Cytotoxicity of a Novel Gallium-Containing Mesoporous Bioactive Glass/Chitosan Composite Scaffold as Hemostatic Agents. ACS Applied Materials & Interfaces, 9(37), pp. 31381-31392. doi:10.1021/acsami.7b07769.
Pourshahrestani S, et al. Potency and Cytotoxicity of a Novel Gallium-Containing Mesoporous Bioactive Glass/Chitosan Composite Scaffold as Hemostatic Agents. ACS Appl Mater Interfaces. 2017 Sep 20;9(37):31381-31392. PubMed PMID: 28836753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potency and Cytotoxicity of a Novel Gallium-Containing Mesoporous Bioactive Glass/Chitosan Composite Scaffold as Hemostatic Agents. AU - Pourshahrestani,Sara, AU - Zeimaran,Ehsan, AU - Kadri,Nahrizul Adib, AU - Gargiulo,Nicola, AU - Jindal,Hassan Mahmood, AU - Naveen,Sangeetha Vasudevaraj, AU - Sekaran,Shamala Devi, AU - Kamarul,Tunku, AU - Towler,Mark R, Y1 - 2017/09/05/ PY - 2017/8/25/pubmed PY - 2019/1/15/medline PY - 2017/8/25/entrez KW - chitosan KW - gallium KW - hemostasis KW - hemostatic property KW - mesoporous bioactive glass SP - 31381 EP - 31392 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 9 IS - 37 N2 - Chitosan-based hemostats are promising candidates for immediate hemorrhage control. However, they have some disadvantages and require further improvement to achieve the desired hemostatic efficiency. Here, a series of 1% Ga2O3-containing mesoporous bioactive glass-chitosan composite scaffolds (Ga-MBG/CHT) were constructed by the lyophilization process and the effect of various concentrations of Ga-MBG (10, 30, and 50 wt %) on the hemostatic function of the CHT scaffold was assessed as compared to that of Celox Rapid gauze (CXR), a current commercially available chitosan-coated hemostatic gauze. The prepared scaffolds exhibited >79% porosity and showed increased water uptake compared to that in CXR. The results of coagulation studies showed that pure CHT and composite scaffolds exhibited increased hemostatic performance with respect to CXR. Furthermore, the composite scaffold with the highest Ga-MBG content (50 wt %) had increased capability to enhancing thrombus generation, blood clotting, and platelet adhesion and aggregation than that of the scaffold made of pure CHT. The antibacterial efficacy and biocompatibility of the prepared scaffolds were also assessed by a time-killing assay and an Alamar Blue assay, respectively. Our results show that the antibacterial effect of 50% Ga-MBG/CHT was more pronounced than that of CHT and CXR. The cell viability results also demonstrated that Ga-MBG/CHT composite scaffolds had good biocompatibility, which facilitates the spreading and proliferation of human dermal fibroblast cells even with 50 wt % Ga-MBG loading. These results suggest that Ga-MBG/CHT scaffolds could be a promising hemostatic candidate for improving hemostasis in critical situations. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/28836753/Potency_and_Cytotoxicity_of_a_Novel_Gallium_Containing_Mesoporous_Bioactive_Glass/Chitosan_Composite_Scaffold_as_Hemostatic_Agents_ L2 - https://dx.doi.org/10.1021/acsami.7b07769 DB - PRIME DP - Unbound Medicine ER -