Tags

Type your tag names separated by a space and hit enter

Neutrophil extracellular traps may contribute to interstitial lung disease associated with anti-MDA5 autoantibody positive dermatomyositis.
Clin Rheumatol 2018; 37(1):107-115CR

Abstract

In dermatomyositis (DM), anti-melanoma differentiation-associated gene 5 (MDA5) autoantibody (autoAb) marks a subtype with low grade or absent muscle inflammation but frequent and rapidly progressive interstitial lung disease (ILD). The pathogenesis of ILD remains poorly unknown. The aim of the study is to explore whether neutrophil extracellular traps (NETs) are involved in the development of ILD in DM patients with anti-MDA5 autoAb. Patients with clinically amyopathic dermatomyositis (CADM, n = 20), classic dermatomyositis (cDM, n = 30), polymyositis (PM, n = 20), and healthy controls (HC, n = 20) were enrolled. Anti-MDA5 autoantibody and Krebs von den Lungen-6 (KL-6) were detected by ELISA. Circulating levels of NETs were assessed by the quantification of both serum cell-free DNA (cfDNA) and LL-37 (cathelicidin LL-37). Immunofluorescent staining was used to visualize NETs ex vivo. The elevated circulating NETs level was detected in DM patients with ILD complication. Compared to anti-MDA5 Ab- DM patients, anti-MDA5 Ab+ DM patients had the higher concentrations of serum cfDNA (293 ± 69 vs 252 ± 63 ng/ml; P = 0.035) and serum LL-37 (0.6 ± 1.0 vs 0.2 ± 0.2 ng/ml; P = 0.026). Positive correlations were established between serum levels of cfDNA and KL-6 in DM patients (r s = 0.4422, P = 0.0003). anti-MDA5 Ab+ sera, other than anti-MDA5 Ab- sera, could induce greater numbers of normal neutrophils to form NETs in vitro. These data suggest that aberrant NETs formation may be involved in the pathogenesis of ILD in DM patients with anti-MDA5 autoAb.

Authors+Show Affiliations

Department of Rheumatology, West China Hospital, Sichuan University, No.37 Guoxue Lane, Chengdu, 610041, China. Department of Rheumatology, The First Affiliated Hospital of Xiamen University, No.55 Zhenhai Road, Xiamen, 361000, China.Department of Rheumatology, West China Hospital, Sichuan University, No.37 Guoxue Lane, Chengdu, 610041, China.Department of Rheumatology, West China Hospital, Sichuan University, No.37 Guoxue Lane, Chengdu, 610041, China.Department of Rheumatology, West China Hospital, Sichuan University, No.37 Guoxue Lane, Chengdu, 610041, China.Department of Rheumatology, West China Hospital, Sichuan University, No.37 Guoxue Lane, Chengdu, 610041, China. yb420@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28842784

Citation

Peng, Yun, et al. "Neutrophil Extracellular Traps May Contribute to Interstitial Lung Disease Associated With anti-MDA5 Autoantibody Positive Dermatomyositis." Clinical Rheumatology, vol. 37, no. 1, 2018, pp. 107-115.
Peng Y, Zhang S, Zhao Y, et al. Neutrophil extracellular traps may contribute to interstitial lung disease associated with anti-MDA5 autoantibody positive dermatomyositis. Clin Rheumatol. 2018;37(1):107-115.
Peng, Y., Zhang, S., Zhao, Y., Liu, Y., & Yan, B. (2018). Neutrophil extracellular traps may contribute to interstitial lung disease associated with anti-MDA5 autoantibody positive dermatomyositis. Clinical Rheumatology, 37(1), pp. 107-115. doi:10.1007/s10067-017-3799-y.
Peng Y, et al. Neutrophil Extracellular Traps May Contribute to Interstitial Lung Disease Associated With anti-MDA5 Autoantibody Positive Dermatomyositis. Clin Rheumatol. 2018;37(1):107-115. PubMed PMID: 28842784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neutrophil extracellular traps may contribute to interstitial lung disease associated with anti-MDA5 autoantibody positive dermatomyositis. AU - Peng,Yun, AU - Zhang,Suhan, AU - Zhao,Yi, AU - Liu,Yi, AU - Yan,Bing, Y1 - 2017/08/25/ PY - 2017/03/30/received PY - 2017/08/18/accepted PY - 2017/08/01/revised PY - 2017/8/27/pubmed PY - 2018/8/4/medline PY - 2017/8/27/entrez KW - Dermatomyositis KW - Interstitial lung disease KW - Melanoma differentiation-associated gene 5 KW - Neutrophil extracellular traps SP - 107 EP - 115 JF - Clinical rheumatology JO - Clin. Rheumatol. VL - 37 IS - 1 N2 - In dermatomyositis (DM), anti-melanoma differentiation-associated gene 5 (MDA5) autoantibody (autoAb) marks a subtype with low grade or absent muscle inflammation but frequent and rapidly progressive interstitial lung disease (ILD). The pathogenesis of ILD remains poorly unknown. The aim of the study is to explore whether neutrophil extracellular traps (NETs) are involved in the development of ILD in DM patients with anti-MDA5 autoAb. Patients with clinically amyopathic dermatomyositis (CADM, n = 20), classic dermatomyositis (cDM, n = 30), polymyositis (PM, n = 20), and healthy controls (HC, n = 20) were enrolled. Anti-MDA5 autoantibody and Krebs von den Lungen-6 (KL-6) were detected by ELISA. Circulating levels of NETs were assessed by the quantification of both serum cell-free DNA (cfDNA) and LL-37 (cathelicidin LL-37). Immunofluorescent staining was used to visualize NETs ex vivo. The elevated circulating NETs level was detected in DM patients with ILD complication. Compared to anti-MDA5 Ab- DM patients, anti-MDA5 Ab+ DM patients had the higher concentrations of serum cfDNA (293 ± 69 vs 252 ± 63 ng/ml; P = 0.035) and serum LL-37 (0.6 ± 1.0 vs 0.2 ± 0.2 ng/ml; P = 0.026). Positive correlations were established between serum levels of cfDNA and KL-6 in DM patients (r s = 0.4422, P = 0.0003). anti-MDA5 Ab+ sera, other than anti-MDA5 Ab- sera, could induce greater numbers of normal neutrophils to form NETs in vitro. These data suggest that aberrant NETs formation may be involved in the pathogenesis of ILD in DM patients with anti-MDA5 autoAb. SN - 1434-9949 UR - https://www.unboundmedicine.com/medline/citation/28842784/Neutrophil_extracellular_traps_may_contribute_to_interstitial_lung_disease_associated_with_anti_MDA5_autoantibody_positive_dermatomyositis_ L2 - https://dx.doi.org/10.1007/s10067-017-3799-y DB - PRIME DP - Unbound Medicine ER -