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Employing proteomic analysis to compare Paracoccidioides lutzii yeast and mycelium cell wall proteins.
Biochim Biophys Acta Proteins Proteom. 2017 Nov; 1865(11 Pt A):1304-1314.BB

Abstract

Paracoccidioidomycosis is an important systemic mycosis caused by thermodimorphic fungi of the Paracoccidioides genus. During the infective process, the cell wall acts at the interface between the fungus and the host. In this way, the cell wall has a key role in growth, environment sensing and interaction, as well as morphogenesis of the fungus. Since the cell wall is absent in mammals, it may present molecules that are described as target sites for new antifungal drugs. Despite its importance, up to now few studies have been conducted employing proteomics in for the identification of cell wall proteins in Paracoccidioides spp. Here, a detailed proteomic approach, including cell wall-fractionation coupled to NanoUPLC-MSE, was used to study and compare the cell wall fractions from Paracoccidioides lutzii mycelia and yeast cells. The analyzed samples consisted of cell wall proteins extracted by hot SDS followed by extraction by mild alkali. In summary, 512 proteins constituting different cell wall fractions were identified, including 7 predicted GPI-dependent cell wall proteins that are potentially involved in cell wall metabolism. Adhesins previously described in Paracoccidioides spp. such as enolase, glyceraldehyde-3-phosphate dehydrogenase were identified. Comparing the proteins in mycelium and yeast cells, we detected some that are common to both fungal phases, such as Ecm33, and some specific proteins, as glucanase Crf1. All of those proteins were described in the metabolism of cell wall. Our study provides an important elucidation of cell wall composition of fractions in Paracoccidioides, opening a way to understand the fungus cell wall architecture.

Authors+Show Affiliations

Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil; Laboratório Interdisciplinar de Biologia, Universidade Estadual de Goiás, Itapuranga, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil.Laboratório de Bioquímica e Química de Proteínas, Instituto de Biologia, Campus Universitário Darci Ribeiro, Brasília, DF, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil. Electronic address: celia@icb.ufg.br.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28844734

Citation

Araújo, Danielle Silva, et al. "Employing Proteomic Analysis to Compare Paracoccidioides Lutzii Yeast and Mycelium Cell Wall Proteins." Biochimica Et Biophysica Acta. Proteins and Proteomics, vol. 1865, no. 11 Pt A, 2017, pp. 1304-1314.
Araújo DS, de Sousa Lima P, Baeza LC, et al. Employing proteomic analysis to compare Paracoccidioides lutzii yeast and mycelium cell wall proteins. Biochim Biophys Acta Proteins Proteom. 2017;1865(11 Pt A):1304-1314.
Araújo, D. S., de Sousa Lima, P., Baeza, L. C., Parente, A. F. A., Melo Bailão, A., Borges, C. L., & de Almeida Soares, C. M. (2017). Employing proteomic analysis to compare Paracoccidioides lutzii yeast and mycelium cell wall proteins. Biochimica Et Biophysica Acta. Proteins and Proteomics, 1865(11 Pt A), 1304-1314. https://doi.org/10.1016/j.bbapap.2017.08.016
Araújo DS, et al. Employing Proteomic Analysis to Compare Paracoccidioides Lutzii Yeast and Mycelium Cell Wall Proteins. Biochim Biophys Acta Proteins Proteom. 2017;1865(11 Pt A):1304-1314. PubMed PMID: 28844734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Employing proteomic analysis to compare Paracoccidioides lutzii yeast and mycelium cell wall proteins. AU - Araújo,Danielle Silva, AU - de Sousa Lima,Patrícia, AU - Baeza,Lilian Cristiane, AU - Parente,Ana Flávia Alves, AU - Melo Bailão,Alexandre, AU - Borges,Clayton Luiz, AU - de Almeida Soares,Célia Maria, Y1 - 2017/08/24/ PY - 2017/01/25/received PY - 2017/08/17/revised PY - 2017/08/21/accepted PY - 2017/8/29/pubmed PY - 2018/2/9/medline PY - 2017/8/29/entrez KW - Cell wall proteins KW - Mycelia KW - Paracoccidioides lutzii KW - Paracoccidioidomycosis KW - Proteomics KW - Yeast cells SP - 1304 EP - 1314 JF - Biochimica et biophysica acta. Proteins and proteomics JO - Biochim Biophys Acta Proteins Proteom VL - 1865 IS - 11 Pt A N2 - Paracoccidioidomycosis is an important systemic mycosis caused by thermodimorphic fungi of the Paracoccidioides genus. During the infective process, the cell wall acts at the interface between the fungus and the host. In this way, the cell wall has a key role in growth, environment sensing and interaction, as well as morphogenesis of the fungus. Since the cell wall is absent in mammals, it may present molecules that are described as target sites for new antifungal drugs. Despite its importance, up to now few studies have been conducted employing proteomics in for the identification of cell wall proteins in Paracoccidioides spp. Here, a detailed proteomic approach, including cell wall-fractionation coupled to NanoUPLC-MSE, was used to study and compare the cell wall fractions from Paracoccidioides lutzii mycelia and yeast cells. The analyzed samples consisted of cell wall proteins extracted by hot SDS followed by extraction by mild alkali. In summary, 512 proteins constituting different cell wall fractions were identified, including 7 predicted GPI-dependent cell wall proteins that are potentially involved in cell wall metabolism. Adhesins previously described in Paracoccidioides spp. such as enolase, glyceraldehyde-3-phosphate dehydrogenase were identified. Comparing the proteins in mycelium and yeast cells, we detected some that are common to both fungal phases, such as Ecm33, and some specific proteins, as glucanase Crf1. All of those proteins were described in the metabolism of cell wall. Our study provides an important elucidation of cell wall composition of fractions in Paracoccidioides, opening a way to understand the fungus cell wall architecture. SN - 1570-9639 UR - https://www.unboundmedicine.com/medline/citation/28844734/Employing_proteomic_analysis_to_compare_Paracoccidioides_lutzii_yeast_and_mycelium_cell_wall_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-9639(17)30202-9 DB - PRIME DP - Unbound Medicine ER -