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New insights into structural organization and gene duplication in a 1.75-Mb genomic region harboring the α-gliadin gene family in Aegilops tauschii, the source of wheat D genome.
Plant J. 2017 Nov; 92(4):571-583.PJ

Abstract

Among the wheat prolamins important for its end-use traits, α-gliadins are the most abundant, and are also a major cause of food-related allergies and intolerances. Previous studies of various wheat species estimated that between 25 and 150 α-gliadin genes reside in the Gli-2 locus regions. To better understand the evolution of this complex gene family, the DNA sequence of a 1.75-Mb genomic region spanning the Gli-2 locus was analyzed in the diploid grass, Aegilops tauschii, the ancestral source of D genome in hexaploid bread wheat. Comparison with orthologous regions from rice, sorghum, and Brachypodium revealed rapid and dynamic changes only occurring to the Ae. tauschii Gli-2 region, including insertions of high numbers of non-syntenic genes and a high rate of tandem gene duplications, the latter of which have given rise to 12 copies of α-gliadin genes clustered within a 550-kb region. Among them, five copies have undergone pseudogenization by various mutation events. Insights into the evolutionary relationship of the duplicated α-gliadin genes were obtained from their genomic organization, transcription patterns, transposable element insertions and phylogenetic analyses. An ancestral glutamate-like receptor (GLR) gene encoding putative amino acid sensor in all four grass species has duplicated only in Ae. tauschii and generated three more copies that are interspersed with the α-gliadin genes. Phylogenetic inference and different gene expression patterns support functional divergence of the Ae. tauschii GLR copies after duplication. Our results suggest that the duplicates of α-gliadin and GLR genes have likely taken different evolutionary paths; conservation for the former and neofunctionalization for the latter.

Authors+Show Affiliations

United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA. Department of Plant Sciences, University of California, Davis, CA, 95616, USA.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA. Henan Institute of Science and Technology, Xinxiang, 453003, China.United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA.Department of Plant Sciences, University of California, Davis, CA, 95616, USA.United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA.United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.Department of Plant Sciences, University of California, Davis, CA, 95616, USA.United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA.Department of Plant Sciences, University of California, Davis, CA, 95616, USA.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.United States Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28857322

Citation

Huo, Naxin, et al. "New Insights Into Structural Organization and Gene Duplication in a 1.75-Mb Genomic Region Harboring the Α-gliadin Gene Family in Aegilops Tauschii, the Source of Wheat D Genome." The Plant Journal : for Cell and Molecular Biology, vol. 92, no. 4, 2017, pp. 571-583.
Huo N, Dong L, Zhang S, et al. New insights into structural organization and gene duplication in a 1.75-Mb genomic region harboring the α-gliadin gene family in Aegilops tauschii, the source of wheat D genome. Plant J. 2017;92(4):571-583.
Huo, N., Dong, L., Zhang, S., Wang, Y., Zhu, T., Mohr, T., Altenbach, S., Liu, Z., Dvorak, J., Anderson, O. D., Luo, M. C., Wang, D., & Gu, Y. Q. (2017). New insights into structural organization and gene duplication in a 1.75-Mb genomic region harboring the α-gliadin gene family in Aegilops tauschii, the source of wheat D genome. The Plant Journal : for Cell and Molecular Biology, 92(4), 571-583. https://doi.org/10.1111/tpj.13675
Huo N, et al. New Insights Into Structural Organization and Gene Duplication in a 1.75-Mb Genomic Region Harboring the Α-gliadin Gene Family in Aegilops Tauschii, the Source of Wheat D Genome. Plant J. 2017;92(4):571-583. PubMed PMID: 28857322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New insights into structural organization and gene duplication in a 1.75-Mb genomic region harboring the α-gliadin gene family in Aegilops tauschii, the source of wheat D genome. AU - Huo,Naxin, AU - Dong,Lingli, AU - Zhang,Shengli, AU - Wang,Yi, AU - Zhu,Tingting, AU - Mohr,Toni, AU - Altenbach,Susan, AU - Liu,Zhiyong, AU - Dvorak,Jan, AU - Anderson,Olin D, AU - Luo,Ming-Cheng, AU - Wang,Daowen, AU - Gu,Yong Q, Y1 - 2017/10/09/ PY - 2017/06/20/received PY - 2017/08/18/revised PY - 2017/08/22/accepted PY - 2017/9/1/pubmed PY - 2018/5/31/medline PY - 2017/9/1/entrez KW - Aegilops tauschii KW - Triticum aestivum KW - gene duplication KW - gene family KW - genome evolution KW - phylogeny KW - promoter KW - wheat prolamins KW - α-gliadin SP - 571 EP - 583 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 92 IS - 4 N2 - Among the wheat prolamins important for its end-use traits, α-gliadins are the most abundant, and are also a major cause of food-related allergies and intolerances. Previous studies of various wheat species estimated that between 25 and 150 α-gliadin genes reside in the Gli-2 locus regions. To better understand the evolution of this complex gene family, the DNA sequence of a 1.75-Mb genomic region spanning the Gli-2 locus was analyzed in the diploid grass, Aegilops tauschii, the ancestral source of D genome in hexaploid bread wheat. Comparison with orthologous regions from rice, sorghum, and Brachypodium revealed rapid and dynamic changes only occurring to the Ae. tauschii Gli-2 region, including insertions of high numbers of non-syntenic genes and a high rate of tandem gene duplications, the latter of which have given rise to 12 copies of α-gliadin genes clustered within a 550-kb region. Among them, five copies have undergone pseudogenization by various mutation events. Insights into the evolutionary relationship of the duplicated α-gliadin genes were obtained from their genomic organization, transcription patterns, transposable element insertions and phylogenetic analyses. An ancestral glutamate-like receptor (GLR) gene encoding putative amino acid sensor in all four grass species has duplicated only in Ae. tauschii and generated three more copies that are interspersed with the α-gliadin genes. Phylogenetic inference and different gene expression patterns support functional divergence of the Ae. tauschii GLR copies after duplication. Our results suggest that the duplicates of α-gliadin and GLR genes have likely taken different evolutionary paths; conservation for the former and neofunctionalization for the latter. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/28857322/New_insights_into_structural_organization_and_gene_duplication_in_a_1_75_Mb_genomic_region_harboring_the_α_gliadin_gene_family_in_Aegilops_tauschii_the_source_of_wheat_D_genome_ L2 - https://doi.org/10.1111/tpj.13675 DB - PRIME DP - Unbound Medicine ER -