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Construction and comparison of different source neuraminidase candidate vaccine strains for human infection with Eurasian avian-like influenza H1N1 virus.
Microbes Infect. 2017 12; 19(12):635-640.MI

Abstract

Human infections with Eurasian avian-like swine influenza H1N1 viruses have been reported in China in past years. One case resulted in death and others were mild case. In 2016, the World Health Organization recommended the use of A/Hunan/42443/2015(H1N1) virus to construct the first candidate vaccine strain for Eurasian avian-like swine influenza H1N1 viruses. Previous reports showed that the neuraminidase of A/Puerto Rico/8/34(H1N1) might improve the viral yield of reassortant viruses. Therefore, we constructed two reassortant candidate vaccine viruses of A/Hunan/42443/2015(H1N1) by reverse genetic technology, with (6+2) and (7+1) gene constitution, respectively. The (6+2) virus had hemagglutinin and neuraminidase from A/Hunan/42443/2015, and the (7+1) one had hemagglutinin from A/Hunan/42443/2015, while all the other genes were from A/Puerto Rico/8/34. Our data revealed that although the neuraminidase of the (7+1) virus was from high yield A/Puerto Rico/8/34, the hemagglutination titer and the hemagglutinin protein content of the (7+1) virus was not higher than that of the (6+2) virus. Both of the (7+1) and (6+2) viruses reached a similar level to that of A/Puerto Rico/8/34 at the usual harvest time in vitro. Therefore, both reassortant viruses are potential candidate vaccine viruses, which could contribute to pandemic preparedness.

Authors+Show Affiliations

Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing, 102206, PR China.Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing, 102206, PR China.Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing, 102206, PR China.Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing, 102206, PR China.Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing, 102206, PR China.Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing, 102206, PR China. Electronic address: dayanwang@cnic.org.cn.Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road, Beijing, 102206, PR China; Public Health School (Shenzhen), Sun Yat-sen University, No.135, Xingang Xi Road, Guangzhou, 510275, PR China. Electronic address: yshu@cnic.org.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28859895

Citation

Liu, Liqi, et al. "Construction and Comparison of Different Source Neuraminidase Candidate Vaccine Strains for Human Infection With Eurasian Avian-like Influenza H1N1 Virus." Microbes and Infection, vol. 19, no. 12, 2017, pp. 635-640.
Liu L, Lu J, Zhou J, et al. Construction and comparison of different source neuraminidase candidate vaccine strains for human infection with Eurasian avian-like influenza H1N1 virus. Microbes Infect. 2017;19(12):635-640.
Liu, L., Lu, J., Zhou, J., Li, Z., Zhang, H., Wang, D., & Shu, Y. (2017). Construction and comparison of different source neuraminidase candidate vaccine strains for human infection with Eurasian avian-like influenza H1N1 virus. Microbes and Infection, 19(12), 635-640. https://doi.org/10.1016/j.micinf.2017.08.004
Liu L, et al. Construction and Comparison of Different Source Neuraminidase Candidate Vaccine Strains for Human Infection With Eurasian Avian-like Influenza H1N1 Virus. Microbes Infect. 2017;19(12):635-640. PubMed PMID: 28859895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction and comparison of different source neuraminidase candidate vaccine strains for human infection with Eurasian avian-like influenza H1N1 virus. AU - Liu,Liqi, AU - Lu,Jian, AU - Zhou,Jianfang, AU - Li,Zi, AU - Zhang,Heng, AU - Wang,Dayan, AU - Shu,Yuelong, Y1 - 2017/08/30/ PY - 2017/06/21/received PY - 2017/08/10/revised PY - 2017/08/22/accepted PY - 2017/9/2/pubmed PY - 2018/7/11/medline PY - 2017/9/2/entrez KW - A (H1N1) KW - Eurasian avian-like influenza KW - Influenza virus KW - Reverse genetic KW - Vaccine SP - 635 EP - 640 JF - Microbes and infection JO - Microbes Infect VL - 19 IS - 12 N2 - Human infections with Eurasian avian-like swine influenza H1N1 viruses have been reported in China in past years. One case resulted in death and others were mild case. In 2016, the World Health Organization recommended the use of A/Hunan/42443/2015(H1N1) virus to construct the first candidate vaccine strain for Eurasian avian-like swine influenza H1N1 viruses. Previous reports showed that the neuraminidase of A/Puerto Rico/8/34(H1N1) might improve the viral yield of reassortant viruses. Therefore, we constructed two reassortant candidate vaccine viruses of A/Hunan/42443/2015(H1N1) by reverse genetic technology, with (6+2) and (7+1) gene constitution, respectively. The (6+2) virus had hemagglutinin and neuraminidase from A/Hunan/42443/2015, and the (7+1) one had hemagglutinin from A/Hunan/42443/2015, while all the other genes were from A/Puerto Rico/8/34. Our data revealed that although the neuraminidase of the (7+1) virus was from high yield A/Puerto Rico/8/34, the hemagglutination titer and the hemagglutinin protein content of the (7+1) virus was not higher than that of the (6+2) virus. Both of the (7+1) and (6+2) viruses reached a similar level to that of A/Puerto Rico/8/34 at the usual harvest time in vitro. Therefore, both reassortant viruses are potential candidate vaccine viruses, which could contribute to pandemic preparedness. SN - 1769-714X UR - https://www.unboundmedicine.com/medline/citation/28859895/Construction_and_comparison_of_different_source_neuraminidase_candidate_vaccine_strains_for_human_infection_with_Eurasian_avian_like_influenza_H1N1_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1286-4579(17)30119-3 DB - PRIME DP - Unbound Medicine ER -