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Analysis of fecal short chain fatty acid concentration in miniature dachshunds with inflammatory colorectal polyps.
J Vet Med Sci. 2017 Oct 20; 79(10):1727-1734.JV

Abstract

Short chain fatty acids (SCFAs) play an important role in the maintenance of colonic homeostasis, and their depletion has been reported in various gastrointestinal disorders. Inflammatory colorectal polyps (ICRPs) are a recently recognized disease specific to miniature dachshunds (MDs), and fecal dysbiosis with a reduction of SCFA-producing bacteria has been reported with this disease. Therefore, this study was performed based on the hypothesis that a reduced SCFA concentration associates with the development of ICRPs. We recruited 11 ICRP-affected MDs and 25 control MDs. Their fecal SCFA concentrations and bacterial proportions were quantified using high performance liquid chromatography and quantitative real-time PCR, respectively. The feces of ICRP-affected MDs contained lower amounts of propionic acid and lower proportions of Bifidobacterium than the feces of control MDs. Furthermore, fecal proportions of Bifidobacterium, Firmicutes and Lactobacillus exhibited significant positive correlations with fecal concentrations of total SCFAs and/or propionic acid; fecal Escherichia coli proportions correlated negatively with fecal concentrations of total SCFAs, as well as acetic, propionic and butyric acid. This result indicates an association between fecal dysbiosis and fecal SCFA concentrations; these phenomena may contribute to ICRP pathogenesis in MDs. Potential therapeutic targeting of the reduced propionic acid concentration using probiotics, prebiotics or SCFA enemas merits further study.

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Authors+Show Affiliations

Igarashi H
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Department of Radiation Biology, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
Ohno K
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Matsuki N
Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Fujiwara-Igarashi A
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Division of Therapeutic Science I, Department of Clinical Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino-shi, Tokyo 180-0023, Japan.
Kanemoto H
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Fukushima K
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Uchida K
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Tsujimoto H
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

MeSH

AnimalsDog DiseasesDogsFatty Acids, VolatileFecesFemaleGastrointestinal MicrobiomeIntestinal PolypsMale

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28867685

Citation

Igarashi, Hirotaka, et al. "Analysis of Fecal Short Chain Fatty Acid Concentration in Miniature Dachshunds With Inflammatory Colorectal Polyps." The Journal of Veterinary Medical Science, vol. 79, no. 10, 2017, pp. 1727-1734.
Igarashi H, Ohno K, Matsuki N, et al. Analysis of fecal short chain fatty acid concentration in miniature dachshunds with inflammatory colorectal polyps. J Vet Med Sci. 2017;79(10):1727-1734.
Igarashi, H., Ohno, K., Matsuki, N., Fujiwara-Igarashi, A., Kanemoto, H., Fukushima, K., Uchida, K., & Tsujimoto, H. (2017). Analysis of fecal short chain fatty acid concentration in miniature dachshunds with inflammatory colorectal polyps. The Journal of Veterinary Medical Science, 79(10), 1727-1734. https://doi.org/10.1292/jvms.17-0165
Igarashi H, et al. Analysis of Fecal Short Chain Fatty Acid Concentration in Miniature Dachshunds With Inflammatory Colorectal Polyps. J Vet Med Sci. 2017 Oct 20;79(10):1727-1734. PubMed PMID: 28867685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of fecal short chain fatty acid concentration in miniature dachshunds with inflammatory colorectal polyps. AU - Igarashi,Hirotaka, AU - Ohno,Koichi, AU - Matsuki,Naoaki, AU - Fujiwara-Igarashi,Aki, AU - Kanemoto,Hideyuki, AU - Fukushima,Kenjiro, AU - Uchida,Kazuyuki, AU - Tsujimoto,Hajime, Y1 - 2017/09/04/ PY - 2017/9/5/pubmed PY - 2018/9/18/medline PY - 2017/9/5/entrez KW - fermentative end product KW - high performance liquid chromatography KW - inflammatory colorectal polyp KW - microflora KW - miniature dachshund SP - 1727 EP - 1734 JF - The Journal of veterinary medical science JO - J Vet Med Sci VL - 79 IS - 10 N2 - Short chain fatty acids (SCFAs) play an important role in the maintenance of colonic homeostasis, and their depletion has been reported in various gastrointestinal disorders. Inflammatory colorectal polyps (ICRPs) are a recently recognized disease specific to miniature dachshunds (MDs), and fecal dysbiosis with a reduction of SCFA-producing bacteria has been reported with this disease. Therefore, this study was performed based on the hypothesis that a reduced SCFA concentration associates with the development of ICRPs. We recruited 11 ICRP-affected MDs and 25 control MDs. Their fecal SCFA concentrations and bacterial proportions were quantified using high performance liquid chromatography and quantitative real-time PCR, respectively. The feces of ICRP-affected MDs contained lower amounts of propionic acid and lower proportions of Bifidobacterium than the feces of control MDs. Furthermore, fecal proportions of Bifidobacterium, Firmicutes and Lactobacillus exhibited significant positive correlations with fecal concentrations of total SCFAs and/or propionic acid; fecal Escherichia coli proportions correlated negatively with fecal concentrations of total SCFAs, as well as acetic, propionic and butyric acid. This result indicates an association between fecal dysbiosis and fecal SCFA concentrations; these phenomena may contribute to ICRP pathogenesis in MDs. Potential therapeutic targeting of the reduced propionic acid concentration using probiotics, prebiotics or SCFA enemas merits further study. SN - 1347-7439 UR - https://www.unboundmedicine.com/medline/citation/28867685/Analysis_of_fecal_short_chain_fatty_acid_concentration_in_miniature_dachshunds_with_inflammatory_colorectal_polyps_ DB - PRIME DP - Unbound Medicine ER -