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Poly-l-cysteine/electrospun copper oxide nanofibers-zinc oxide nanoparticles nanocomposite as sensing element of an electrochemical sensor for simultaneous determination of adenine and guanine in biological samples and evaluation of damage to dsDNA and DNA purine bases by UV radiation.
Anal Chim Acta. 2017 Sep 15; 986:25-41.AC

Abstract

A new nanocomposite film constructed of poly-l-cysteine/zinc oxide nanoparticles-electrospun copper oxide nanofibers (PLC/ZnO-NPs-CuO-NFs) was prepared on the surface of the graphite electrode (GE). The novel electrode was successfully applied for the simultaneous determination of guanine (G) and adenine (A), two of the most important components of DNA and RNA. The PLC/ZnO-NPs-CuO-NFs/GE enhanced the anodic peak currents of the purine bases conspicuously and could determine them sensitively and separately in 0.1 M phosphate buffer solution at the physiological pH (7.0). The synthesized nanofibers, nanoparticles and nanocomposite were characterized by different methods such as Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray diffraction (XRD) and energy dispersive X-ray analysis (EDS). Under the optimum operating conditions, linear calibration curves were obtained in the range of 0.05-6.78 and 0.01-3.87 μM with a detection limit of 12.48 and 1.25 nM for G and A, respectively. The proposed method was applied to quantify A and G in three different DNA samples with satisfactory results. In addition, damage to human blood double-stranded DNA (dsDNA) and DNA purine bases (liberated in previously hydrolyzed human blood dsDNA) caused by UV-C and UV-B were evaluated. The results demonstrated that the proposed biosensing platform not only provides a novel and sensitive approach to detecting DNA damage, but also can be used for simultaneous determination of purine bases and major products of DNA oxidative damage.

Authors+Show Affiliations

Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914-41335, Rasht, Iran. Electronic address: arvand@guilan.ac.ir.Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914-41335, Rasht, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28870323

Citation

Arvand, Majid, and Masoomeh Sayyar Ardaki. "Poly-l-cysteine/electrospun Copper Oxide Nanofibers-zinc Oxide Nanoparticles Nanocomposite as Sensing Element of an Electrochemical Sensor for Simultaneous Determination of Adenine and Guanine in Biological Samples and Evaluation of Damage to dsDNA and DNA Purine Bases By UV Radiation." Analytica Chimica Acta, vol. 986, 2017, pp. 25-41.
Arvand M, Sayyar Ardaki M. Poly-l-cysteine/electrospun copper oxide nanofibers-zinc oxide nanoparticles nanocomposite as sensing element of an electrochemical sensor for simultaneous determination of adenine and guanine in biological samples and evaluation of damage to dsDNA and DNA purine bases by UV radiation. Anal Chim Acta. 2017;986:25-41.
Arvand, M., & Sayyar Ardaki, M. (2017). Poly-l-cysteine/electrospun copper oxide nanofibers-zinc oxide nanoparticles nanocomposite as sensing element of an electrochemical sensor for simultaneous determination of adenine and guanine in biological samples and evaluation of damage to dsDNA and DNA purine bases by UV radiation. Analytica Chimica Acta, 986, 25-41. https://doi.org/10.1016/j.aca.2017.07.057
Arvand M, Sayyar Ardaki M. Poly-l-cysteine/electrospun Copper Oxide Nanofibers-zinc Oxide Nanoparticles Nanocomposite as Sensing Element of an Electrochemical Sensor for Simultaneous Determination of Adenine and Guanine in Biological Samples and Evaluation of Damage to dsDNA and DNA Purine Bases By UV Radiation. Anal Chim Acta. 2017 Sep 15;986:25-41. PubMed PMID: 28870323.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poly-l-cysteine/electrospun copper oxide nanofibers-zinc oxide nanoparticles nanocomposite as sensing element of an electrochemical sensor for simultaneous determination of adenine and guanine in biological samples and evaluation of damage to dsDNA and DNA purine bases by UV radiation. AU - Arvand,Majid, AU - Sayyar Ardaki,Masoomeh, Y1 - 2017/07/29/ PY - 2017/04/28/received PY - 2017/07/20/revised PY - 2017/07/24/accepted PY - 2017/9/6/entrez PY - 2017/9/6/pubmed PY - 2018/7/3/medline KW - Adenine KW - DNA damage KW - Electrospun copper oxide nanofibers KW - Guanine KW - Poly-l-cysteine KW - Zinc oxide nanoparticles SP - 25 EP - 41 JF - Analytica chimica acta JO - Anal Chim Acta VL - 986 N2 - A new nanocomposite film constructed of poly-l-cysteine/zinc oxide nanoparticles-electrospun copper oxide nanofibers (PLC/ZnO-NPs-CuO-NFs) was prepared on the surface of the graphite electrode (GE). The novel electrode was successfully applied for the simultaneous determination of guanine (G) and adenine (A), two of the most important components of DNA and RNA. The PLC/ZnO-NPs-CuO-NFs/GE enhanced the anodic peak currents of the purine bases conspicuously and could determine them sensitively and separately in 0.1 M phosphate buffer solution at the physiological pH (7.0). The synthesized nanofibers, nanoparticles and nanocomposite were characterized by different methods such as Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray diffraction (XRD) and energy dispersive X-ray analysis (EDS). Under the optimum operating conditions, linear calibration curves were obtained in the range of 0.05-6.78 and 0.01-3.87 μM with a detection limit of 12.48 and 1.25 nM for G and A, respectively. The proposed method was applied to quantify A and G in three different DNA samples with satisfactory results. In addition, damage to human blood double-stranded DNA (dsDNA) and DNA purine bases (liberated in previously hydrolyzed human blood dsDNA) caused by UV-C and UV-B were evaluated. The results demonstrated that the proposed biosensing platform not only provides a novel and sensitive approach to detecting DNA damage, but also can be used for simultaneous determination of purine bases and major products of DNA oxidative damage. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/28870323/Poly_l_cysteine/electrospun_copper_oxide_nanofibers_zinc_oxide_nanoparticles_nanocomposite_as_sensing_element_of_an_electrochemical_sensor_for_simultaneous_determination_of_adenine_and_guanine_in_biological_samples_and_evaluation_of_damage_to_dsDNA_and_DNA_purine_bases_by_UV_radiation_ DB - PRIME DP - Unbound Medicine ER -