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Assessing the Detection of Middle East Respiratory Syndrome Coronavirus IgG in Suspected and Proven Cases of Middle East Respiratory Syndrome Coronavirus Infection.
Viral Immunol. 2017 11; 30(9):649-653.VI

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease with significant mortality. Two testing methods are currently used for MERS-CoV diagnostics: nucleic acid detection (the gold standard) and serological analysis. In this study, we evaluated the detection of MERS-CoV-IgG in suspected and proven cases of MERS-CoV infection. We enrolled 174 patients: 113 had respiratory symptoms/suspected MERS-CoV infection, 31 had confirmed influenza A or B infection, 23 had a recent confirmed MERS-CoV infection, and 7 had confirmed MERS-CoV infection 1 year before. All underwent MERS-CoV RNA and MERS-CoV-IgG testing. Thirty patients were found to be MERS-CoV RNA positive; however, during serological analysis, only 6 (3.4%) patients were positive for MERS-CoV-IgG, 1 (0.6%) patient was equivocal, and 167 (96%) patients were negative. Among the serological positives, four were recently MERS-CoV RNA positive and two were MERS-CoV RNA negative. No cross-reactivity to influenza A or B was detected. Based on the lack of correlation between nucleic acid and serological analysis, we conclude that MERS-CoV-IgG testing may not be suitable for diagnosing acute infection or estimating its prevalence during an outbreak. In addition, our findings show that MERS-CoV-IgG may not have significant value in determining disease severity or prognosis.

Authors+Show Affiliations

1 King Khalid University Hospital , Riyadh, Saudi Arabia . 2 Department of Pathology, College of Medicine, King Saud University , Riyadh, Saudi Arabia .1 King Khalid University Hospital , Riyadh, Saudi Arabia . 3 Department of Infectious Diseases, College of Medicine, King Saud University , Riyadh, Saudi Arabia .1 King Khalid University Hospital , Riyadh, Saudi Arabia . 2 Department of Pathology, College of Medicine, King Saud University , Riyadh, Saudi Arabia .1 King Khalid University Hospital , Riyadh, Saudi Arabia . 2 Department of Pathology, College of Medicine, King Saud University , Riyadh, Saudi Arabia .1 King Khalid University Hospital , Riyadh, Saudi Arabia .1 King Khalid University Hospital , Riyadh, Saudi Arabia . 2 Department of Pathology, College of Medicine, King Saud University , Riyadh, Saudi Arabia .1 King Khalid University Hospital , Riyadh, Saudi Arabia . 2 Department of Pathology, College of Medicine, King Saud University , Riyadh, Saudi Arabia .1 King Khalid University Hospital , Riyadh, Saudi Arabia . 3 Department of Infectious Diseases, College of Medicine, King Saud University , Riyadh, Saudi Arabia .4 King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia .4 King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28873020

Citation

Alhetheel, Abdulkarim, et al. "Assessing the Detection of Middle East Respiratory Syndrome Coronavirus IgG in Suspected and Proven Cases of Middle East Respiratory Syndrome Coronavirus Infection." Viral Immunology, vol. 30, no. 9, 2017, pp. 649-653.
Alhetheel A, Altalhi H, Albarrag A, et al. Assessing the Detection of Middle East Respiratory Syndrome Coronavirus IgG in Suspected and Proven Cases of Middle East Respiratory Syndrome Coronavirus Infection. Viral Immunol. 2017;30(9):649-653.
Alhetheel, A., Altalhi, H., Albarrag, A., Shakoor, Z., Mohamed, D., El-Hazmi, M., Somily, A., Barry, M., Bakhrebah, M., & Nassar, M. (2017). Assessing the Detection of Middle East Respiratory Syndrome Coronavirus IgG in Suspected and Proven Cases of Middle East Respiratory Syndrome Coronavirus Infection. Viral Immunology, 30(9), 649-653. https://doi.org/10.1089/vim.2017.0091
Alhetheel A, et al. Assessing the Detection of Middle East Respiratory Syndrome Coronavirus IgG in Suspected and Proven Cases of Middle East Respiratory Syndrome Coronavirus Infection. Viral Immunol. 2017;30(9):649-653. PubMed PMID: 28873020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessing the Detection of Middle East Respiratory Syndrome Coronavirus IgG in Suspected and Proven Cases of Middle East Respiratory Syndrome Coronavirus Infection. AU - Alhetheel,Abdulkarim, AU - Altalhi,Haifa, AU - Albarrag,Ahmed, AU - Shakoor,Zahid, AU - Mohamed,Deqa, AU - El-Hazmi,Malak, AU - Somily,Ali, AU - Barry,Mazin, AU - Bakhrebah,Muhammed, AU - Nassar,Majed, Y1 - 2017/09/05/ PY - 2017/9/6/pubmed PY - 2018/9/19/medline PY - 2017/9/6/entrez KW - MERS diagnostic testing KW - MERS-CoV RNA KW - MERS-CoV-IgG SP - 649 EP - 653 JF - Viral immunology JO - Viral Immunol VL - 30 IS - 9 N2 - Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease with significant mortality. Two testing methods are currently used for MERS-CoV diagnostics: nucleic acid detection (the gold standard) and serological analysis. In this study, we evaluated the detection of MERS-CoV-IgG in suspected and proven cases of MERS-CoV infection. We enrolled 174 patients: 113 had respiratory symptoms/suspected MERS-CoV infection, 31 had confirmed influenza A or B infection, 23 had a recent confirmed MERS-CoV infection, and 7 had confirmed MERS-CoV infection 1 year before. All underwent MERS-CoV RNA and MERS-CoV-IgG testing. Thirty patients were found to be MERS-CoV RNA positive; however, during serological analysis, only 6 (3.4%) patients were positive for MERS-CoV-IgG, 1 (0.6%) patient was equivocal, and 167 (96%) patients were negative. Among the serological positives, four were recently MERS-CoV RNA positive and two were MERS-CoV RNA negative. No cross-reactivity to influenza A or B was detected. Based on the lack of correlation between nucleic acid and serological analysis, we conclude that MERS-CoV-IgG testing may not be suitable for diagnosing acute infection or estimating its prevalence during an outbreak. In addition, our findings show that MERS-CoV-IgG may not have significant value in determining disease severity or prognosis. SN - 1557-8976 UR - https://www.unboundmedicine.com/medline/citation/28873020/Assessing_the_Detection_of_Middle_East_Respiratory_Syndrome_Coronavirus_IgG_in_Suspected_and_Proven_Cases_of_Middle_East_Respiratory_Syndrome_Coronavirus_Infection_ L2 - https://www.liebertpub.com/doi/10.1089/vim.2017.0091?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -