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Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels.
Sci Rep 2017; 7(1):10509SR

Abstract

We investigated cytosolic calcium (Ca2+) and sarcoplasmic reticulum Ca2+ regulation in skeletal muscle fibers of hibernating Daurian ground squirrels (Spermophilus dauricus), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventing muscle atrophy in hibernators. The Na+, K+-ATPase and Ca2+-ATPase activities in the soleus muscle (SOL) of squirrels were maintained in hibernation, decreased during interbout arousal (IB-A), and increased to autumn/pre-hibernation (AUT/Pre-H) levels in torpor after interbout arousal (Post-IBA), whereas activities in the extensor digitorum longus muscle (EDL) were stable during hibernation, but increased during post-hibernation (Post-H). Activities increased in the SOL of HLU rats, but were stable in HLU squirrels. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in the SOL decreased in IB-A squirrels, but returned to AUT/Pre-H levels in the Post-IBA group; no significant changes were found in the EDL. SERCA activity increased in the EDL of HLU squirrels and SOL of HLU rats. Compared with AUT/Pre-H, SERCA type 2 protein expression increased in the SOL and EDL of IB-A and Post-IBA squirrels, but increased in the SOL only in HLU animals. We also describe the protein kinase A changes in this paper. Thus, hibernating ground squirrels displayed remarkable Na+, K+-ATPase, Ca2+-ATPase, and SERCA plasticity.

Authors+Show Affiliations

Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China. Shaanxi Sci-Tech University, School of Biological Science and Engineering, Hanzhong, 723001, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, 710069, China. gaoyunf@nwu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28874726

Citation

Guo, Quanling, et al. "Remarkable Plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA Contributes to Muscle Disuse Atrophy Resistance in Hibernating Daurian Ground Squirrels." Scientific Reports, vol. 7, no. 1, 2017, p. 10509.
Guo Q, Mi X, Sun X, et al. Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels. Sci Rep. 2017;7(1):10509.
Guo, Q., Mi, X., Sun, X., Li, X., Fu, W., Xu, S., ... Gao, Y. (2017). Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels. Scientific Reports, 7(1), p. 10509. doi:10.1038/s41598-017-10829-6.
Guo Q, et al. Remarkable Plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA Contributes to Muscle Disuse Atrophy Resistance in Hibernating Daurian Ground Squirrels. Sci Rep. 2017 09 5;7(1):10509. PubMed PMID: 28874726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels. AU - Guo,Quanling, AU - Mi,Xin, AU - Sun,Xiaoyong, AU - Li,Xiaoyu, AU - Fu,Weiwei, AU - Xu,Shenhui, AU - Wang,Qi, AU - Arfat,Yasir, AU - Wang,Huiping, AU - Chang,Hui, AU - Gao,Yunfang, Y1 - 2017/09/05/ PY - 2017/03/13/received PY - 2017/08/16/accepted PY - 2017/9/7/entrez PY - 2017/9/7/pubmed PY - 2019/5/10/medline SP - 10509 EP - 10509 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - We investigated cytosolic calcium (Ca2+) and sarcoplasmic reticulum Ca2+ regulation in skeletal muscle fibers of hibernating Daurian ground squirrels (Spermophilus dauricus), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventing muscle atrophy in hibernators. The Na+, K+-ATPase and Ca2+-ATPase activities in the soleus muscle (SOL) of squirrels were maintained in hibernation, decreased during interbout arousal (IB-A), and increased to autumn/pre-hibernation (AUT/Pre-H) levels in torpor after interbout arousal (Post-IBA), whereas activities in the extensor digitorum longus muscle (EDL) were stable during hibernation, but increased during post-hibernation (Post-H). Activities increased in the SOL of HLU rats, but were stable in HLU squirrels. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in the SOL decreased in IB-A squirrels, but returned to AUT/Pre-H levels in the Post-IBA group; no significant changes were found in the EDL. SERCA activity increased in the EDL of HLU squirrels and SOL of HLU rats. Compared with AUT/Pre-H, SERCA type 2 protein expression increased in the SOL and EDL of IB-A and Post-IBA squirrels, but increased in the SOL only in HLU animals. We also describe the protein kinase A changes in this paper. Thus, hibernating ground squirrels displayed remarkable Na+, K+-ATPase, Ca2+-ATPase, and SERCA plasticity. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28874726/Remarkable_plasticity_of_Na+_K+_ATPase_Ca2+_ATPase_and_SERCA_contributes_to_muscle_disuse_atrophy_resistance_in_hibernating_Daurian_ground_squirrels_ L2 - http://dx.doi.org/10.1038/s41598-017-10829-6 DB - PRIME DP - Unbound Medicine ER -