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Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis.
JDR Clin Trans Res. 2017 Jul; 2(3):258-268.JC

Abstract

Localized aggressive periodontitis (LAP) patients possess a systemic hyperinflammatory response after lipopolysaccharide stimulation. However, the levels of inflammatory and bone biomarkers in plasma, as well as possible associations between local and plasma biomarkers, are unknown in LAP. This cross-sectional study aimed to characterize gingival crevicular fluid (GCF) and plasma biomarker profiles in LAP patients, their healthy siblings (HS), and healthy unrelated controls (HC). Fifty-eight LAP subjects, 33 HS, and 49 HC (African Americans, aged 5 to 25 y) were included. Following collection of clinical parameters with GCF and plasma samples, levels of 16 inflammatory and bone resorption biomarkers were determined with Milliplex. Univariate and correlation analyses were performed among all clinical and laboratorial parameters. Discriminant analyses were used to investigate groups of biomarkers discriminating LAP from HS and HC in GCF and plasma. GCF levels of multiple cytokines and chemokines and RANKL:OPG ratio (receptor activator of nuclear factor kappa-B ligand:osteoprotegerin) were higher in LAP disease, most of which positively correlated with probing depth and attachment loss of sampled sites. A group of IL-12p40, IL-6, IL-12p70, IL-2, and MIP-1α discriminated LAP diseased sites from twheir healthy sites, as well as from HS and HC healthy sites. In plasma, only RANKL levels were increased in LAP versus controls, which positively correlated with the percentage of affected sites and deep/bleeding sites. A plasma inflammatory profile including MIP-1α, IL-8, IL-10, and INF-γ could significantly discriminate LAP patients from HS and HC. No correlations were found between GCF and plasma levels of biomarkers. In conclusion, an inflammatory profile including groups of specific biomarkers in GCF and plasma may significantly discriminate LAP from healthy individuals. The hyperinflammatory response previously found in the peripheral blood of LAP patients is dependent on lipopolysaccharide stimulation, apparently resulting mostly in local tissue destruction and changes in biomarker profile, with a slight influence in the systemic inflammatory profile (ClinicalTrials.gov NCT01330719). Knowledge Transfer Statement: The results of this study can be possibly used by clinicians in the future as diagnostic tools for localized aggressive periodontitis. Thus, in the future, with proper consideration of cost, patient preference, chair-side feasibility and ultimately further studies validating the role of GCF markers for disease progression and response to treatment, this information could lead to more appropriate therapeutic decisions and the development of preventive approaches for susceptible patients.

Authors+Show Affiliations

Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. Department of Dentistry II, School of Dentistry, Federal University of Maranhão, São Luís, Brazil.Pain Research and Intervention Center of Excellence, University of Florida, Gainesville, FL, USA.Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. Department of Periodontology, Trinity College School of Dental Sciences, Dublin, Ireland.Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA.Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28879248

Citation

Branco-de-Almeida, L S., et al. "Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis." JDR Clinical and Translational Research, vol. 2, no. 3, 2017, pp. 258-268.
Branco-de-Almeida LS, Cruz-Almeida Y, Gonzalez-Marrero Y, et al. Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis. JDR Clin Trans Res. 2017;2(3):258-268.
Branco-de-Almeida, L. S., Cruz-Almeida, Y., Gonzalez-Marrero, Y., Huang, H., Aukhil, I., Harrison, P., Wallet, S. M., & Shaddox, L. M. (2017). Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis. JDR Clinical and Translational Research, 2(3), 258-268. https://doi.org/10.1177/2380084417701898
Branco-de-Almeida LS, et al. Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis. JDR Clin Trans Res. 2017;2(3):258-268. PubMed PMID: 28879248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Local and Plasma Biomarker Profiles in Localized Aggressive Periodontitis. AU - Branco-de-Almeida,L S, AU - Cruz-Almeida,Y, AU - Gonzalez-Marrero,Y, AU - Huang,H, AU - Aukhil,I, AU - Harrison,P, AU - Wallet,S M, AU - Shaddox,L M, Y1 - 2017/04/14/ PY - 2017/9/8/entrez PY - 2017/9/8/pubmed PY - 2017/9/8/medline KW - blood KW - bone resorption KW - cytokines KW - gingival crevicular fluid KW - inflammation KW - periodontal diseases SP - 258 EP - 268 JF - JDR clinical and translational research JO - JDR Clin Trans Res VL - 2 IS - 3 N2 - Localized aggressive periodontitis (LAP) patients possess a systemic hyperinflammatory response after lipopolysaccharide stimulation. However, the levels of inflammatory and bone biomarkers in plasma, as well as possible associations between local and plasma biomarkers, are unknown in LAP. This cross-sectional study aimed to characterize gingival crevicular fluid (GCF) and plasma biomarker profiles in LAP patients, their healthy siblings (HS), and healthy unrelated controls (HC). Fifty-eight LAP subjects, 33 HS, and 49 HC (African Americans, aged 5 to 25 y) were included. Following collection of clinical parameters with GCF and plasma samples, levels of 16 inflammatory and bone resorption biomarkers were determined with Milliplex. Univariate and correlation analyses were performed among all clinical and laboratorial parameters. Discriminant analyses were used to investigate groups of biomarkers discriminating LAP from HS and HC in GCF and plasma. GCF levels of multiple cytokines and chemokines and RANKL:OPG ratio (receptor activator of nuclear factor kappa-B ligand:osteoprotegerin) were higher in LAP disease, most of which positively correlated with probing depth and attachment loss of sampled sites. A group of IL-12p40, IL-6, IL-12p70, IL-2, and MIP-1α discriminated LAP diseased sites from twheir healthy sites, as well as from HS and HC healthy sites. In plasma, only RANKL levels were increased in LAP versus controls, which positively correlated with the percentage of affected sites and deep/bleeding sites. A plasma inflammatory profile including MIP-1α, IL-8, IL-10, and INF-γ could significantly discriminate LAP patients from HS and HC. No correlations were found between GCF and plasma levels of biomarkers. In conclusion, an inflammatory profile including groups of specific biomarkers in GCF and plasma may significantly discriminate LAP from healthy individuals. The hyperinflammatory response previously found in the peripheral blood of LAP patients is dependent on lipopolysaccharide stimulation, apparently resulting mostly in local tissue destruction and changes in biomarker profile, with a slight influence in the systemic inflammatory profile (ClinicalTrials.gov NCT01330719). Knowledge Transfer Statement: The results of this study can be possibly used by clinicians in the future as diagnostic tools for localized aggressive periodontitis. Thus, in the future, with proper consideration of cost, patient preference, chair-side feasibility and ultimately further studies validating the role of GCF markers for disease progression and response to treatment, this information could lead to more appropriate therapeutic decisions and the development of preventive approaches for susceptible patients. SN - 2380-0844 UR - https://www.unboundmedicine.com/medline/citation/28879248/Local_and_Plasma_Biomarker_Profiles_in_Localized_Aggressive_Periodontitis_ L2 - http://journals.sagepub.com/doi/full/10.1177/2380084417701898?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -