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Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood.
J Am Soc Mass Spectrom. 2017 12; 28(12):2665-2676.JA

Abstract

Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. Graphical abstract ᅟ.

Authors+Show Affiliations

ThoMSon Mass Spectrometry Laboratory, University of Campinas - UNICAMP, Campinas, 13083-970, Brazil. Department of Chemistry and Center for Analytical Instrumentation, Purdue University, West Lafayette, IN, 47907, USA.Department of Chemistry and Center for Analytical Instrumentation, Purdue University, West Lafayette, IN, 47907, USA.RIKILT, Wageningen Research, Akkermaalsbos 2, 6708 WB, P.O. Box 230, 6700 AE, Wageningen, The Netherlands.RIKILT, Wageningen Research, Akkermaalsbos 2, 6708 WB, P.O. Box 230, 6700 AE, Wageningen, The Netherlands. Laboratory of Organic Chemistry, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.ThoMSon Mass Spectrometry Laboratory, University of Campinas - UNICAMP, Campinas, 13083-970, Brazil.Department of Chemistry and Center for Analytical Instrumentation, Purdue University, West Lafayette, IN, 47907, USA.Netherlands Forensic Institute, P.O. Box 24044, 2490 AA, The Hague, The Netherlands. a.van.asten@nfi.minvenj.nl. Faculty of Science, Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, P.O. Box 94157, 1090 GD, Amsterdam, The Netherlands. a.van.asten@nfi.minvenj.nl. CLHC, Amsterdam Center for Forensic Science and Medicine, P.O. Box 94157, 1090 GD, Amsterdam, The Netherlands. a.van.asten@nfi.minvenj.nl.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

28879579

Citation

Teunissen, Sebastiaan F., et al. "Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood." Journal of the American Society for Mass Spectrometry, vol. 28, no. 12, 2017, pp. 2665-2676.
Teunissen SF, Fedick PW, Berendsen BJA, et al. Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood. J Am Soc Mass Spectrom. 2017;28(12):2665-2676.
Teunissen, S. F., Fedick, P. W., Berendsen, B. J. A., Nielen, M. W. F., Eberlin, M. N., Graham Cooks, R., & van Asten, A. C. (2017). Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood. Journal of the American Society for Mass Spectrometry, 28(12), 2665-2676. https://doi.org/10.1007/s13361-017-1790-0
Teunissen SF, et al. Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood. J Am Soc Mass Spectrom. 2017;28(12):2665-2676. PubMed PMID: 28879579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood. AU - Teunissen,Sebastiaan F, AU - Fedick,Patrick W, AU - Berendsen,Bjorn J A, AU - Nielen,Michel W F, AU - Eberlin,Marcos N, AU - Graham Cooks,R, AU - van Asten,Arian C, Y1 - 2017/09/06/ PY - 2017/06/26/received PY - 2017/08/10/accepted PY - 2017/08/09/revised PY - 2017/9/8/pubmed PY - 2019/5/24/medline PY - 2017/9/8/entrez KW - Amphetamines KW - Forensic toxicology KW - Paper spray mass spectrometry KW - Selectivity KW - Validation KW - Whole blood SP - 2665 EP - 2676 JF - Journal of the American Society for Mass Spectrometry JO - J Am Soc Mass Spectrom VL - 28 IS - 12 N2 - Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. Graphical abstract ᅟ. SN - 1879-1123 UR - https://www.unboundmedicine.com/medline/citation/28879579/Novel_Selectivity_Based_Forensic_Toxicological_Validation_of_a_Paper_Spray_Mass_Spectrometry_Method_for_the_Quantitative_Determination_of_Eight_Amphetamines_in_Whole_Blood_ L2 - https://antibodies.cancer.gov/detail/CPTC-FANCA-1 DB - PRIME DP - Unbound Medicine ER -