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Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts.
Molecules. 2017 Sep 07; 22(9)M

Abstract

Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations.

Authors+Show Affiliations

Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. tmdgml7898@naver.com.Laboratory of Toxicology, College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Korea. shki@chosun.ac.kr.Department of Nursing, Dongshin University, Naju-si, Jeonnam 58245, Korea. dhj1221@hanmail.net.Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. hbsmoon@mokpo.ac.kr.Department of Business Administration, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. leecdai@mokpo.ac.kr.Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Geumjeong-gu, Busan 46241, Korea. insoo.yoon@pusan.ac.kr.Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea. sscho@mokpo.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28880226

Citation

Song, Seung-Hui, et al. "Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania Tricuspidata Leaf and Fruit Extracts." Molecules (Basel, Switzerland), vol. 22, no. 9, 2017.
Song SH, Ki SH, Park DH, et al. Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts. Molecules. 2017;22(9).
Song, S. H., Ki, S. H., Park, D. H., Moon, H. S., Lee, C. D., Yoon, I. S., & Cho, S. S. (2017). Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts. Molecules (Basel, Switzerland), 22(9). https://doi.org/10.3390/molecules22091489
Song SH, et al. Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania Tricuspidata Leaf and Fruit Extracts. Molecules. 2017 Sep 7;22(9) PubMed PMID: 28880226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts. AU - Song,Seung-Hui, AU - Ki,Sung Hwan, AU - Park,Dae-Hun, AU - Moon,Hong-Seop, AU - Lee,Chang-Dai, AU - Yoon,In-Soo, AU - Cho,Seung-Sik, Y1 - 2017/09/07/ PY - 2017/08/22/received PY - 2017/09/05/accepted PY - 2017/9/8/entrez PY - 2017/9/8/pubmed PY - 2018/7/7/medline KW - C. tricuspidata Bureau KW - HPLC KW - hyperuricemia KW - simultaneous analysis KW - xanthine oxidase JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 9 N2 - Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/28880226/Quantitative_Analysis_Extraction_Optimization_and_Biological_Evaluation_of_Cudrania_tricuspidata_Leaf_and_Fruit_Extracts_ L2 - http://www.mdpi.com/resolver?pii=molecules22091489 DB - PRIME DP - Unbound Medicine ER -