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Improved fruit α-tocopherol, carotenoid, squalene and phytosterol contents through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato.
Plant Biotechnol J. 2018 03; 16(3):784-796.PB

Abstract

3-Hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS) in the mevalonate (MVA) pathway generates isoprenoids including phytosterols. Dietary phytosterols are important because they can lower blood cholesterol levels. Previously, the overexpression of Brassica juncea wild-type (wt) and mutant (S359A) BjHMGS1 in Arabidopsis up-regulated several genes in sterol biosynthesis and increased sterol content. Recombinant S359A had earlier displayed a 10-fold higher in vitro enzyme activity. Furthermore, tobacco HMGS overexpressors (OEs) exhibited improved sterol content, plant growth and seed yield. Increased growth and seed yield in tobacco OE-S359A over OE-wtBjHMGS1 coincided with elevations in NtSQS expression and sterol content. Herein, the overexpression of wt and mutant (S359A) BjHMGS1 in a crop plant, tomato (Solanum lycopersicum), caused an accumulation of MVA-derived squalene and phytosterols, as well as methylerythritol phosphate (MEP)-derived α-tocopherol (vitamin E) and carotenoids, which are important to human health as antioxidants. In tomato HMGS-OE seedlings, genes associated with the biosyntheses of C10, C15 and C20 universal precursors of isoprenoids, phytosterols, brassinosteroids, dolichols, methylerythritol phosphate, carotenoid and vitamin E were up-regulated. In OE-S359A tomato fruits, increased squalene and phytosterol contents over OE-wtBjHMGS1 were attributed to heightened SlHMGR2, SlFPS1, SlSQS and SlCYP710A11 expression. In both tomato OE-wtBjHMGS1 and OE-S359A fruits, the up-regulation of SlGPS and SlGGPPS1 in the MEP pathway that led to α-tocopherol and carotenoid accumulation indicated cross-talk between the MVA and MEP pathways. Taken together, the manipulation of BjHMGS1 represents a promising strategy to simultaneously elevate health-promoting squalene, phytosterols, α-tocopherol and carotenoids in tomato, an edible fruit.

Authors+Show Affiliations

School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China. Partner State Key Laboratory of Agrobiotechnology, CUHK, Shatin, Hong Kong, China.School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.Centre National de la Recherche Scientifique, UPR 2357, Institut de Biologie Moléculaire des Plantes, Strasbourg, France.School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China. Partner State Key Laboratory of Agrobiotechnology, CUHK, Shatin, Hong Kong, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28881416

Citation

Liao, Pan, et al. "Improved Fruit Α-tocopherol, Carotenoid, Squalene and Phytosterol Contents Through Manipulation of Brassica Juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in Transgenic Tomato." Plant Biotechnology Journal, vol. 16, no. 3, 2018, pp. 784-796.
Liao P, Chen X, Wang M, et al. Improved fruit α-tocopherol, carotenoid, squalene and phytosterol contents through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato. Plant Biotechnol J. 2018;16(3):784-796.
Liao, P., Chen, X., Wang, M., Bach, T. J., & Chye, M. L. (2018). Improved fruit α-tocopherol, carotenoid, squalene and phytosterol contents through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato. Plant Biotechnology Journal, 16(3), 784-796. https://doi.org/10.1111/pbi.12828
Liao P, et al. Improved Fruit Α-tocopherol, Carotenoid, Squalene and Phytosterol Contents Through Manipulation of Brassica Juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in Transgenic Tomato. Plant Biotechnol J. 2018;16(3):784-796. PubMed PMID: 28881416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved fruit α-tocopherol, carotenoid, squalene and phytosterol contents through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato. AU - Liao,Pan, AU - Chen,Xinjian, AU - Wang,Mingfu, AU - Bach,Thomas J, AU - Chye,Mee-Len, Y1 - 2017/10/17/ PY - 2017/04/24/received PY - 2017/08/20/revised PY - 2017/08/26/accepted PY - 2017/9/8/pubmed PY - 2019/1/3/medline PY - 2017/9/8/entrez KW - 3-hydroxy-3-methylglutaryl-coenzyme A synthase KW - isoprenoids KW - mevalonate KW - phytosterols KW - squalene KW - tomato SP - 784 EP - 796 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 16 IS - 3 N2 - 3-Hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS) in the mevalonate (MVA) pathway generates isoprenoids including phytosterols. Dietary phytosterols are important because they can lower blood cholesterol levels. Previously, the overexpression of Brassica juncea wild-type (wt) and mutant (S359A) BjHMGS1 in Arabidopsis up-regulated several genes in sterol biosynthesis and increased sterol content. Recombinant S359A had earlier displayed a 10-fold higher in vitro enzyme activity. Furthermore, tobacco HMGS overexpressors (OEs) exhibited improved sterol content, plant growth and seed yield. Increased growth and seed yield in tobacco OE-S359A over OE-wtBjHMGS1 coincided with elevations in NtSQS expression and sterol content. Herein, the overexpression of wt and mutant (S359A) BjHMGS1 in a crop plant, tomato (Solanum lycopersicum), caused an accumulation of MVA-derived squalene and phytosterols, as well as methylerythritol phosphate (MEP)-derived α-tocopherol (vitamin E) and carotenoids, which are important to human health as antioxidants. In tomato HMGS-OE seedlings, genes associated with the biosyntheses of C10, C15 and C20 universal precursors of isoprenoids, phytosterols, brassinosteroids, dolichols, methylerythritol phosphate, carotenoid and vitamin E were up-regulated. In OE-S359A tomato fruits, increased squalene and phytosterol contents over OE-wtBjHMGS1 were attributed to heightened SlHMGR2, SlFPS1, SlSQS and SlCYP710A11 expression. In both tomato OE-wtBjHMGS1 and OE-S359A fruits, the up-regulation of SlGPS and SlGGPPS1 in the MEP pathway that led to α-tocopherol and carotenoid accumulation indicated cross-talk between the MVA and MEP pathways. Taken together, the manipulation of BjHMGS1 represents a promising strategy to simultaneously elevate health-promoting squalene, phytosterols, α-tocopherol and carotenoids in tomato, an edible fruit. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/28881416/Improved_fruit_α_tocopherol_carotenoid_squalene_and_phytosterol_contents_through_manipulation_of_Brassica_juncea_3_HYDROXY_3_METHYLGLUTARYL_COA_SYNTHASE1_in_transgenic_tomato_ L2 - https://doi.org/10.1111/pbi.12828 DB - PRIME DP - Unbound Medicine ER -