Tags

Type your tag names separated by a space and hit enter

Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo-A comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet.
PLoS One 2017; 12(9):e0184470Plos

Abstract

Dietary intervention and genetic fat-1 mice are two models for the investigation of effects associated with omega-3 polyunsaturated fatty acids (n3-PUFA). In order to assess their power to modulate the fatty acid and oxylipin pattern, we thoroughly compared fat-1 and wild-type C57BL/6 mice on a sunflower oil diet with wild-type mice on the same diet enriched with 1% EPA and 1% DHA for 0, 7, 14, 30 and 45 days. Feeding led after 14-30 days to a high steady state of n3-PUFA in all tissues at the expense of n6-PUFAs. Levels of n3-PUFA achieved by feeding were higher compared to fat-1 mice, particularly for EPA (max. 1.7% in whole blood of fat-1 vs. 7.8% following feeding). Changes in PUFAs were reflected in most oxylipins in plasma, brain and colon: Compared to wild-type mice on a standard diet, arachidonic acid metabolites were overall decreased while EPA and DHA oxylipins increased with feeding more than in fat-1 mice. In plasma of n3-PUFA fed animals, EPA and DHA metabolites from the lipoxygenase and cytochrome P450 pathways dominated over ARA derived counterparts.Fat-1 mice show n3-PUFA level which can be reached by dietary interventions, supporting the applicability of this model in n3-PUFA research. However, for specific questions, e.g. the role of EPA derived mediators or concentration dependent effects of (individual) PUFA, feeding studies are necessary.

Authors+Show Affiliations

Institute for Food Toxicology, University of Veterinary Medicine Hannover, Hannover, Germany. Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany.Institute for Food Toxicology, University of Veterinary Medicine Hannover, Hannover, Germany. Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.Institute for Food Toxicology, University of Veterinary Medicine Hannover, Hannover, Germany.Medical Department, Division of Hepatology and Gastroenterology (including Metabolic Diseases), Charité University Medicine Berlin, Campus Virchow Klinikum, Berlin, Germany.Medical Department, Division of Hepatology and Gastroenterology (including Metabolic Diseases), Charité University Medicine Berlin, Campus Virchow Klinikum, Berlin, Germany.Medical Department, Division of Hepatology and Gastroenterology (including Metabolic Diseases), Charité University Medicine Berlin, Campus Virchow Klinikum, Berlin, Germany.Medical Department, Division of Hepatology and Gastroenterology (including Metabolic Diseases), Charité University Medicine Berlin, Campus Virchow Klinikum, Berlin, Germany. Experimental and Clinical Research Centre, Charité University Medicine, Campus Buch, Berlin, Germany. Medical Department, Division of Gastroenterology, Oncology, Hematology, Rheumatology and Diabetes, Ruppiner Kliniken, Brandenburg Medical School, Neuruppin, Germany.Institute for Food Toxicology, University of Veterinary Medicine Hannover, Hannover, Germany. Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28886129

Citation

Ostermann, Annika I., et al. "Modulation of the Endogenous Omega-3 Fatty Acid and Oxylipin Profile in vivo-A Comparison of the Fat-1 Transgenic Mouse With C57BL/6 Wildtype Mice On an Omega-3 Fatty Acid Enriched Diet." PloS One, vol. 12, no. 9, 2017, pp. e0184470.
Ostermann AI, Waindok P, Schmidt MJ, et al. Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo-A comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet. PLoS ONE. 2017;12(9):e0184470.
Ostermann, A. I., Waindok, P., Schmidt, M. J., Chiu, C. Y., Smyl, C., Rohwer, N., ... Schebb, N. H. (2017). Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo-A comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet. PloS One, 12(9), pp. e0184470. doi:10.1371/journal.pone.0184470.
Ostermann AI, et al. Modulation of the Endogenous Omega-3 Fatty Acid and Oxylipin Profile in vivo-A Comparison of the Fat-1 Transgenic Mouse With C57BL/6 Wildtype Mice On an Omega-3 Fatty Acid Enriched Diet. PLoS ONE. 2017;12(9):e0184470. PubMed PMID: 28886129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo-A comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet. AU - Ostermann,Annika I, AU - Waindok,Patrick, AU - Schmidt,Moritz J, AU - Chiu,Cheng-Ying, AU - Smyl,Christopher, AU - Rohwer,Nadine, AU - Weylandt,Karsten-H, AU - Schebb,Nils Helge, Y1 - 2017/09/08/ PY - 2017/05/12/received PY - 2017/08/24/accepted PY - 2017/9/9/entrez PY - 2017/9/9/pubmed PY - 2017/10/19/medline SP - e0184470 EP - e0184470 JF - PloS one JO - PLoS ONE VL - 12 IS - 9 N2 - Dietary intervention and genetic fat-1 mice are two models for the investigation of effects associated with omega-3 polyunsaturated fatty acids (n3-PUFA). In order to assess their power to modulate the fatty acid and oxylipin pattern, we thoroughly compared fat-1 and wild-type C57BL/6 mice on a sunflower oil diet with wild-type mice on the same diet enriched with 1% EPA and 1% DHA for 0, 7, 14, 30 and 45 days. Feeding led after 14-30 days to a high steady state of n3-PUFA in all tissues at the expense of n6-PUFAs. Levels of n3-PUFA achieved by feeding were higher compared to fat-1 mice, particularly for EPA (max. 1.7% in whole blood of fat-1 vs. 7.8% following feeding). Changes in PUFAs were reflected in most oxylipins in plasma, brain and colon: Compared to wild-type mice on a standard diet, arachidonic acid metabolites were overall decreased while EPA and DHA oxylipins increased with feeding more than in fat-1 mice. In plasma of n3-PUFA fed animals, EPA and DHA metabolites from the lipoxygenase and cytochrome P450 pathways dominated over ARA derived counterparts.Fat-1 mice show n3-PUFA level which can be reached by dietary interventions, supporting the applicability of this model in n3-PUFA research. However, for specific questions, e.g. the role of EPA derived mediators or concentration dependent effects of (individual) PUFA, feeding studies are necessary. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28886129/Modulation_of_the_endogenous_omega_3_fatty_acid_and_oxylipin_profile_in_vivo_A_comparison_of_the_fat_1_transgenic_mouse_with_C57BL/6_wildtype_mice_on_an_omega_3_fatty_acid_enriched_diet_ L2 - http://dx.plos.org/10.1371/journal.pone.0184470 DB - PRIME DP - Unbound Medicine ER -