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Impaired platelet aggregation and thromboxane generation in essential fatty acid-deficient rats.
J Nutr. 1987 Sep; 117(9):1520-6.JN

Abstract

ADP-induced platelet aggregation and thrombin-induced thromboxane B2 generation in diluted whole blood from rats fed a fat-free diet supplemented with 10% (by weight) hydrogenated coconut oil [essential fatty acid (EFA) deficient] were significantly lower than that in animals fed 10% safflower oil [(SFO) rich in linoleic acid] or 10% marine oil (rich in eicosapentaenoic acid and docosahexaenoic acid). Plasma fibrinogen levels were significantly lower and liver function was impaired in the EFA-deficient group compared with the other two groups. Platelet responsiveness to ADP was restored when plasma from the EFA-deficient rats was replaced by plasma obtained from rats fed a nonpurified diet. ADP responsiveness and thrombin-stimulated thromboxane B2 production in diluted whole blood were also restored after 2 wk of injections of 100 mg ethyl linoleate every 48 h, and partially restored by injections of 100 mg ethyl alpha-linolenate. When ADP-induced platelet aggregation was examined in washed platelets, the impaired ADP aggregation (found when platelets of the EFA-deficient rats were suspended in their own plasma) was not observed at either high (9.5 microM) or low (1.0 microM) ADP concentrations. Although thrombin-stimulated thromboxane B2 production in washed platelets in the EFA-deficient rats was lower than that in the SFO-fed rats, the magnitude of aggregation was not different. In addition, inhibition of thrombin-induced platelet aggregation by apyrase (0.06 U/mL) was identical in the two groups. These results suggest that impaired platelet aggregation in EFA deficiency is related more to plasma factors than to inherent platelet properties and that restoration of normal liver function is associated with normal platelet function.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Efamol Research Institute, Kentville, Nova Scotia, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2888839

Citation

Takahashi, R, et al. "Impaired Platelet Aggregation and Thromboxane Generation in Essential Fatty Acid-deficient Rats." The Journal of Nutrition, vol. 117, no. 9, 1987, pp. 1520-6.
Takahashi R, Manku MS, Horrobin DF. Impaired platelet aggregation and thromboxane generation in essential fatty acid-deficient rats. J Nutr. 1987;117(9):1520-6.
Takahashi, R., Manku, M. S., & Horrobin, D. F. (1987). Impaired platelet aggregation and thromboxane generation in essential fatty acid-deficient rats. The Journal of Nutrition, 117(9), 1520-6.
Takahashi R, Manku MS, Horrobin DF. Impaired Platelet Aggregation and Thromboxane Generation in Essential Fatty Acid-deficient Rats. J Nutr. 1987;117(9):1520-6. PubMed PMID: 2888839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired platelet aggregation and thromboxane generation in essential fatty acid-deficient rats. AU - Takahashi,R, AU - Manku,M S, AU - Horrobin,D F, PY - 1987/9/1/pubmed PY - 1987/9/1/medline PY - 1987/9/1/entrez SP - 1520 EP - 6 JF - The Journal of nutrition JO - J Nutr VL - 117 IS - 9 N2 - ADP-induced platelet aggregation and thrombin-induced thromboxane B2 generation in diluted whole blood from rats fed a fat-free diet supplemented with 10% (by weight) hydrogenated coconut oil [essential fatty acid (EFA) deficient] were significantly lower than that in animals fed 10% safflower oil [(SFO) rich in linoleic acid] or 10% marine oil (rich in eicosapentaenoic acid and docosahexaenoic acid). Plasma fibrinogen levels were significantly lower and liver function was impaired in the EFA-deficient group compared with the other two groups. Platelet responsiveness to ADP was restored when plasma from the EFA-deficient rats was replaced by plasma obtained from rats fed a nonpurified diet. ADP responsiveness and thrombin-stimulated thromboxane B2 production in diluted whole blood were also restored after 2 wk of injections of 100 mg ethyl linoleate every 48 h, and partially restored by injections of 100 mg ethyl alpha-linolenate. When ADP-induced platelet aggregation was examined in washed platelets, the impaired ADP aggregation (found when platelets of the EFA-deficient rats were suspended in their own plasma) was not observed at either high (9.5 microM) or low (1.0 microM) ADP concentrations. Although thrombin-stimulated thromboxane B2 production in washed platelets in the EFA-deficient rats was lower than that in the SFO-fed rats, the magnitude of aggregation was not different. In addition, inhibition of thrombin-induced platelet aggregation by apyrase (0.06 U/mL) was identical in the two groups. These results suggest that impaired platelet aggregation in EFA deficiency is related more to plasma factors than to inherent platelet properties and that restoration of normal liver function is associated with normal platelet function.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0022-3166 UR - https://www.unboundmedicine.com/medline/citation/2888839/Impaired_platelet_aggregation_and_thromboxane_generation_in_essential_fatty_acid_deficient_rats_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.1093/jn/117.9.1520 DB - PRIME DP - Unbound Medicine ER -