Tags

Type your tag names separated by a space and hit enter

Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates.
J Ind Microbiol Biotechnol. 2017 Nov; 44(11):1575-1588.JI

Abstract

An industrial ethanol-producing Saccharomyces cerevisiae strain with genes of fungal oxido-reductive pathway needed for xylose fermentation integrated into its genome (YRH1415) was used to obtain haploids and diploid isogenic strains. The isogenic strains were more effective in metabolizing xylose than YRH1415 strain and able to co-ferment glucose and xylose in the presence of high concentrations of inhibitors resulting from the hydrolysis of lignocellulosic biomass (switchgrass). The rate of xylose consumption did not appear to be affected by the ploidy of strains or the presence of two copies of the xylose fermentation genes but by heterozygosity of alleles for xylose metabolism in YRH1415. Furthermore, inhibitor tolerance was influenced by the heterozygous genome of the industrial strain, which also showed a marked influenced on tolerance to increasing concentrations of toxic compounds, such as furfural. In this work, selection of haploid derivatives was found to be a useful strategy to develop efficient xylose-fermenting industrial yeast strains.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Lopes DD
Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
Rosa CA
Biological Science Institute, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Hector RE
Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-Agricultural Research Service, Peoria, IL, USA.
Dien BS
Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-Agricultural Research Service, Peoria, IL, USA.
Mertens JA
Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-Agricultural Research Service, Peoria, IL, USA.
Ayub MAZ
Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. mazayub@ufrgs.br.

MeSH

BiomassCloning, MolecularCulture MediaEthanolFermentationFuraldehydeGene Expression Regulation, FungalGenetic BackgroundGlucoseHydrolysisLigninSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsXylose

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28891041

Citation

Lopes, Daiane Dias, et al. "Influence of Genetic Background of Engineered Xylose-fermenting Industrial Saccharomyces Cerevisiae Strains for Ethanol Production From Lignocellulosic Hydrolysates." Journal of Industrial Microbiology & Biotechnology, vol. 44, no. 11, 2017, pp. 1575-1588.
Lopes DD, Rosa CA, Hector RE, et al. Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates. J Ind Microbiol Biotechnol. 2017;44(11):1575-1588.
Lopes, D. D., Rosa, C. A., Hector, R. E., Dien, B. S., Mertens, J. A., & Ayub, M. A. Z. (2017). Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates. Journal of Industrial Microbiology & Biotechnology, 44(11), 1575-1588. https://doi.org/10.1007/s10295-017-1979-z
Lopes DD, et al. Influence of Genetic Background of Engineered Xylose-fermenting Industrial Saccharomyces Cerevisiae Strains for Ethanol Production From Lignocellulosic Hydrolysates. J Ind Microbiol Biotechnol. 2017;44(11):1575-1588. PubMed PMID: 28891041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates. AU - Lopes,Daiane Dias, AU - Rosa,Carlos Augusto, AU - Hector,Ronald E, AU - Dien,Bruce S, AU - Mertens,Jeffrey A, AU - Ayub,Marco Antônio Záchia, Y1 - 2017/09/11/ PY - 2017/04/29/received PY - 2017/09/05/accepted PY - 2017/9/12/pubmed PY - 2018/4/6/medline PY - 2017/9/12/entrez KW - Furfural KW - PE-2 industrial Saccharomyces cerevisiae strain KW - Second generation ethanol KW - Switchgrass biomass hydrolysate KW - Xylose pathway SP - 1575 EP - 1588 JF - Journal of industrial microbiology & biotechnology JO - J Ind Microbiol Biotechnol VL - 44 IS - 11 N2 - An industrial ethanol-producing Saccharomyces cerevisiae strain with genes of fungal oxido-reductive pathway needed for xylose fermentation integrated into its genome (YRH1415) was used to obtain haploids and diploid isogenic strains. The isogenic strains were more effective in metabolizing xylose than YRH1415 strain and able to co-ferment glucose and xylose in the presence of high concentrations of inhibitors resulting from the hydrolysis of lignocellulosic biomass (switchgrass). The rate of xylose consumption did not appear to be affected by the ploidy of strains or the presence of two copies of the xylose fermentation genes but by heterozygosity of alleles for xylose metabolism in YRH1415. Furthermore, inhibitor tolerance was influenced by the heterozygous genome of the industrial strain, which also showed a marked influenced on tolerance to increasing concentrations of toxic compounds, such as furfural. In this work, selection of haploid derivatives was found to be a useful strategy to develop efficient xylose-fermenting industrial yeast strains. SN - 1476-5535 UR - https://www.unboundmedicine.com/medline/citation/28891041/Influence_of_genetic_background_of_engineered_xylose_fermenting_industrial_Saccharomyces_cerevisiae_strains_for_ethanol_production_from_lignocellulosic_hydrolysates_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓6 ↑32]

Related Citations

More