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Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd).
BMC Complement Altern Med. 2017 Sep 11; 17(1):457.BC

Abstract

BACKGROUND

Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its traditional claims, antioxidant and anti-proliferative activities were evaluated in the present study.

METHODS

Ethanol extract (TCE) and subsequent petroleum ether (TCP), dichloromethane (TCD), n-Butanol (TCB) and aqueous (TCA) fractions of were prepared from stems of T cordifolia. Total phenolic, flavonoid content and anti-oxidant activity was assessed by different methods. Anti-proliferative activity was assessed in cervical carcinoma (HeLa) cell lines by MTT and SRB assay.

RESULTS

Ethanol extract and n-butanol fractions shown to be superior in their scavenging activity in all the tested methods. n-butanol fractions shown antioxidant activity with an IC50 of 14.81 ± 0.53, 29.48 ± 2.23, 58.20 ± 0.70 and 21.17 ± 1.19 μg/mL by DPPH, ABTS, Nitric oxide and iron chelating activities respectively. Anti-proliferative activity results demonstrates that the TCD and ethanol extract of T cordifolia exhibits potent cytotoxic effect against HeLa with an IC50 of 54.23 ± 0.94 μg/mL and 101.26 ± 1.42 μg/mL respectively by MTT assay; and with an IC50 of 48.91 ± 0.33 μg/mL and 87.93 ± 0.85 μg/mL respectively by SRB assay.

CONCLUSION

The outcomes of the present study support the fact that T Cordifolia is a promising source of antioxidant agent and propose its further investigation. Moreover, dichloromethane fraction of T cordifolia shown to be the most potent anti-proliferative fraction and further mechanistic and phytochemical investigations are under way to identify the active principles.

Authors+Show Affiliations

Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, -576104, India.Directorate of Research (Health Sciences), Manipal University, Manipal, Karnataka, 576104, India. n.udupa@manipal.edu.Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, -576104, India.Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, -576104, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28893230

Citation

Polu, Picheswara Rao, et al. "Assessment of Free Radical Scavenging and Anti-proliferative Activities of Tinospora Cordifolia Miers (Willd)." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 457.
Polu PR, Nayanbhirama U, Khan S, et al. Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd). BMC Complement Altern Med. 2017;17(1):457.
Polu, P. R., Nayanbhirama, U., Khan, S., & Maheswari, R. (2017). Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd). BMC Complementary and Alternative Medicine, 17(1), 457. https://doi.org/10.1186/s12906-017-1953-3
Polu PR, et al. Assessment of Free Radical Scavenging and Anti-proliferative Activities of Tinospora Cordifolia Miers (Willd). BMC Complement Altern Med. 2017 Sep 11;17(1):457. PubMed PMID: 28893230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd). AU - Polu,Picheswara Rao, AU - Nayanbhirama,Udupa, AU - Khan,Saleemulla, AU - Maheswari,Rajlexmi, Y1 - 2017/09/11/ PY - 2017/05/10/received PY - 2017/08/30/accepted PY - 2017/9/13/entrez PY - 2017/9/13/pubmed PY - 2017/9/19/medline KW - Anti-oxidant KW - Berberine KW - Cervical carcinoma KW - SRB assay KW - Tinospora cordifolia SP - 457 EP - 457 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its traditional claims, antioxidant and anti-proliferative activities were evaluated in the present study. METHODS: Ethanol extract (TCE) and subsequent petroleum ether (TCP), dichloromethane (TCD), n-Butanol (TCB) and aqueous (TCA) fractions of were prepared from stems of T cordifolia. Total phenolic, flavonoid content and anti-oxidant activity was assessed by different methods. Anti-proliferative activity was assessed in cervical carcinoma (HeLa) cell lines by MTT and SRB assay. RESULTS: Ethanol extract and n-butanol fractions shown to be superior in their scavenging activity in all the tested methods. n-butanol fractions shown antioxidant activity with an IC50 of 14.81 ± 0.53, 29.48 ± 2.23, 58.20 ± 0.70 and 21.17 ± 1.19 μg/mL by DPPH, ABTS, Nitric oxide and iron chelating activities respectively. Anti-proliferative activity results demonstrates that the TCD and ethanol extract of T cordifolia exhibits potent cytotoxic effect against HeLa with an IC50 of 54.23 ± 0.94 μg/mL and 101.26 ± 1.42 μg/mL respectively by MTT assay; and with an IC50 of 48.91 ± 0.33 μg/mL and 87.93 ± 0.85 μg/mL respectively by SRB assay. CONCLUSION: The outcomes of the present study support the fact that T Cordifolia is a promising source of antioxidant agent and propose its further investigation. Moreover, dichloromethane fraction of T cordifolia shown to be the most potent anti-proliferative fraction and further mechanistic and phytochemical investigations are under way to identify the active principles. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28893230/Assessment_of_free_radical_scavenging_and_anti_proliferative_activities_of_Tinospora_cordifolia_Miers__Willd__ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1953-3 DB - PRIME DP - Unbound Medicine ER -