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Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering.
Biomater Sci. 2017 Sep 26; 5(10):2024-2034.BS

Abstract

Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine. Herein, conductive poly(3,4-ethylenedioxythiophene) doped with hyaluronic acid (PEDOT-HA) nanoparticles were firstly synthesized via chemical oxidant polymerization. A three-dimensional (3D) PEDOT-HA/Cs/Gel scaffold was then developed by introducing PEDOT-HA nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. HA, as a bridge, not only was used as a dopant, but also combined PEDOT into the Cs/Gel via chemical crosslinking. The PEDOT-HA/Cs/Gel scaffold was used as a conductive substrate for neural stem cell (NSC) culture in vitro. The results demonstrated that the PEDOT-HA/Cs/Gel scaffold had excellent biocompatibility for NSC proliferation and differentiation. 3D confocal fluorescence images showed cells attached on the channel surface of Cs/Gel and PEDOT-HA/Cs/Gel scaffolds with a normal neuronal morphology. Compared to the Cs/Gel scaffold, the PEDOT-HA/Cs/Gel scaffold not only promoted NSC proliferation with up-regulated expression of Ki67, but also enhanced NSC differentiation into neurons and astrocytes with up-regulated expression of β tubulin-III and GFAP, respectively. It is expected that this electro-active and bio-active PEDOT-HA/Cs/Gel scaffold will be used as a conductive platform to regulate NSC behavior for neural tissue engineering.

Authors+Show Affiliations

Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, People's Republic of China. guanshui@dlut.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28894864

Citation

Wang, Shuping, et al. "Neural Stem Cell Proliferation and Differentiation in the Conductive PEDOT-HA/Cs/Gel Scaffold for Neural Tissue Engineering." Biomaterials Science, vol. 5, no. 10, 2017, pp. 2024-2034.
Wang S, Guan S, Xu J, et al. Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering. Biomater Sci. 2017;5(10):2024-2034.
Wang, S., Guan, S., Xu, J., Li, W., Ge, D., Sun, C., Liu, T., & Ma, X. (2017). Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering. Biomaterials Science, 5(10), 2024-2034. https://doi.org/10.1039/c7bm00633k
Wang S, et al. Neural Stem Cell Proliferation and Differentiation in the Conductive PEDOT-HA/Cs/Gel Scaffold for Neural Tissue Engineering. Biomater Sci. 2017 Sep 26;5(10):2024-2034. PubMed PMID: 28894864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering. AU - Wang,Shuping, AU - Guan,Shui, AU - Xu,Jianqiang, AU - Li,Wenfang, AU - Ge,Dan, AU - Sun,Changkai, AU - Liu,Tianqing, AU - Ma,Xuehu, PY - 2017/9/13/pubmed PY - 2018/5/17/medline PY - 2017/9/13/entrez SP - 2024 EP - 2034 JF - Biomaterials science JO - Biomater Sci VL - 5 IS - 10 N2 - Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine. Herein, conductive poly(3,4-ethylenedioxythiophene) doped with hyaluronic acid (PEDOT-HA) nanoparticles were firstly synthesized via chemical oxidant polymerization. A three-dimensional (3D) PEDOT-HA/Cs/Gel scaffold was then developed by introducing PEDOT-HA nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. HA, as a bridge, not only was used as a dopant, but also combined PEDOT into the Cs/Gel via chemical crosslinking. The PEDOT-HA/Cs/Gel scaffold was used as a conductive substrate for neural stem cell (NSC) culture in vitro. The results demonstrated that the PEDOT-HA/Cs/Gel scaffold had excellent biocompatibility for NSC proliferation and differentiation. 3D confocal fluorescence images showed cells attached on the channel surface of Cs/Gel and PEDOT-HA/Cs/Gel scaffolds with a normal neuronal morphology. Compared to the Cs/Gel scaffold, the PEDOT-HA/Cs/Gel scaffold not only promoted NSC proliferation with up-regulated expression of Ki67, but also enhanced NSC differentiation into neurons and astrocytes with up-regulated expression of β tubulin-III and GFAP, respectively. It is expected that this electro-active and bio-active PEDOT-HA/Cs/Gel scaffold will be used as a conductive platform to regulate NSC behavior for neural tissue engineering. SN - 2047-4849 UR - https://www.unboundmedicine.com/medline/citation/28894864/Neural_stem_cell_proliferation_and_differentiation_in_the_conductive_PEDOT_HA/Cs/Gel_scaffold_for_neural_tissue_engineering_ L2 - https://doi.org/10.1039/c7bm00633k DB - PRIME DP - Unbound Medicine ER -