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Combined Effect of Ultrasound and Mild Temperatures on the Inactivation of E. coli in Fresh Carrot Juice and Changes on its Physicochemical Characteristics.
J Food Sci. 2017 Oct; 82(10):2343-2350.JF

Abstract

The combination of ultrasound and mild temperatures to process fruits and vegetables juices is a novel approach that is showing promising results for microbial inactivation and preservation of bioactive compounds and sensory attributes. This study centers on investigating the inactivation of Escherichia coli (ATCC 11755) in carrot juice as a result of the combined effect of ultrasound (24 kHz frequency, 120 μm, and 400 W) with temperature (50, 54, and 58 °C) and processing time (0 to 10 min). In addition, the possible changes in physicochemical properties and the retention of bioactive compounds after processing were analyzed. Microbial inactivation with ultrasound treatment at 50 °C resulted in 3.5 log reduction after 10 min, whereas at 54 °C almost 5 log reduction was attained in the same period of time; meanwhile, for treatment at 58 °C, no viable cells were detected (>5 log reduction) after 2 min. There was no significant difference (P > 0.05) on pH (6.80 to 6.82), °Brix (8.0 to 8.5), titratable acidity (0.29% to 0.30%), total carotenoid (1774 to 1835 μg/100 mL), phenolic compounds (20.19 to 20.63 μg/mL), ascorbic acid (4.8 mg/100 mL), and color parameters between fresh and ultrasound treated samples at the studied temperatures. To predict the inactivation patterns, observed values were tested using 3 different general models: first-order, Weibull distribution, and biphasic. The Weibull and biphasic models show good correlation for inactivation under all processing conditions. Results show ultrasound in combination with mild temperature could be effectively used to process fresh carrot juice providing a safe product without affecting physicochemical characteristics.

PRACTICAL APPLICATIONS

The combination of ultrasound and mild temperatures is effective in reducing microbial load in carrot juice to safe levels. This combination would be beneficial in the industrial processing of carrot juice without altering the quality attributes or bioactive compounds.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Pokhrel PR
Center for Nonthermal Processing of Food, Washington State Univ., Pullman, Wash., 99164-6120, U.S.A. Dept. of Biological Systems Engineering, Washington State Univ., Pullman, Wash, 99164-6120, U.S.A.
Bermúdez-Aguirre D
Center for Nonthermal Processing of Food, Washington State Univ., Pullman, Wash., 99164-6120, U.S.A. Dept. of Biological Systems Engineering, Washington State Univ., Pullman, Wash, 99164-6120, U.S.A.
Martínez-Flores HE
Center for Nonthermal Processing of Food, Washington State Univ., Pullman, Wash., 99164-6120, U.S.A. Dept. of Biological Systems Engineering, Washington State Univ., Pullman, Wash, 99164-6120, U.S.A. Faculty of Chemical Pharmacobiology, Michoacana Univ., St. Nicholas, Mexico.
Garnica-Romo MG
Center for Nonthermal Processing of Food, Washington State Univ., Pullman, Wash., 99164-6120, U.S.A. Dept. of Biological Systems Engineering, Washington State Univ., Pullman, Wash, 99164-6120, U.S.A. Faculty of Civil Engineering, Michoacana Univ., St. Nicholas, Mexico.
Sablani S
Dept. of Biological Systems Engineering, Washington State Univ., Pullman, Wash, 99164-6120, U.S.A.
Tang J
Dept. of Biological Systems Engineering, Washington State Univ., Pullman, Wash, 99164-6120, U.S.A.
Barbosa-Cánovas GV
Center for Nonthermal Processing of Food, Washington State Univ., Pullman, Wash., 99164-6120, U.S.A. Dept. of Biological Systems Engineering, Washington State Univ., Pullman, Wash, 99164-6120, U.S.A.

MeSH

Ascorbic AcidCarotenoidsDaucus carotaEscherichia coliFood ContaminationFood HandlingFood QualityFruit and Vegetable JuicesMicrobial ViabilityPhenolsTemperatureUltrasonics

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28898409

Citation

Pokhrel, Prashant Raj, et al. "Combined Effect of Ultrasound and Mild Temperatures On the Inactivation of E. Coli in Fresh Carrot Juice and Changes On Its Physicochemical Characteristics." Journal of Food Science, vol. 82, no. 10, 2017, pp. 2343-2350.
Pokhrel PR, Bermúdez-Aguirre D, Martínez-Flores HE, et al. Combined Effect of Ultrasound and Mild Temperatures on the Inactivation of E. coli in Fresh Carrot Juice and Changes on its Physicochemical Characteristics. J Food Sci. 2017;82(10):2343-2350.
Pokhrel, P. R., Bermúdez-Aguirre, D., Martínez-Flores, H. E., Garnica-Romo, M. G., Sablani, S., Tang, J., & Barbosa-Cánovas, G. V. (2017). Combined Effect of Ultrasound and Mild Temperatures on the Inactivation of E. coli in Fresh Carrot Juice and Changes on its Physicochemical Characteristics. Journal of Food Science, 82(10), 2343-2350. https://doi.org/10.1111/1750-3841.13787
Pokhrel PR, et al. Combined Effect of Ultrasound and Mild Temperatures On the Inactivation of E. Coli in Fresh Carrot Juice and Changes On Its Physicochemical Characteristics. J Food Sci. 2017;82(10):2343-2350. PubMed PMID: 28898409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined Effect of Ultrasound and Mild Temperatures on the Inactivation of E. coli in Fresh Carrot Juice and Changes on its Physicochemical Characteristics. AU - Pokhrel,Prashant Raj, AU - Bermúdez-Aguirre,Daniela, AU - Martínez-Flores,Héctor E, AU - Garnica-Romo,M Guadalupe, AU - Sablani,Shyam, AU - Tang,Juming, AU - Barbosa-Cánovas,Gustavo V, Y1 - 2017/09/12/ PY - 2017/02/16/received PY - 2017/05/06/revised PY - 2017/05/15/accepted PY - 2017/9/13/pubmed PY - 2018/3/7/medline PY - 2017/9/13/entrez KW - Escherichia coli KW - carrot juice KW - mild-pasteurization KW - nonthermal processing KW - ultrasound SP - 2343 EP - 2350 JF - Journal of food science JO - J Food Sci VL - 82 IS - 10 N2 - : The combination of ultrasound and mild temperatures to process fruits and vegetables juices is a novel approach that is showing promising results for microbial inactivation and preservation of bioactive compounds and sensory attributes. This study centers on investigating the inactivation of Escherichia coli (ATCC 11755) in carrot juice as a result of the combined effect of ultrasound (24 kHz frequency, 120 μm, and 400 W) with temperature (50, 54, and 58 °C) and processing time (0 to 10 min). In addition, the possible changes in physicochemical properties and the retention of bioactive compounds after processing were analyzed. Microbial inactivation with ultrasound treatment at 50 °C resulted in 3.5 log reduction after 10 min, whereas at 54 °C almost 5 log reduction was attained in the same period of time; meanwhile, for treatment at 58 °C, no viable cells were detected (>5 log reduction) after 2 min. There was no significant difference (P > 0.05) on pH (6.80 to 6.82), °Brix (8.0 to 8.5), titratable acidity (0.29% to 0.30%), total carotenoid (1774 to 1835 μg/100 mL), phenolic compounds (20.19 to 20.63 μg/mL), ascorbic acid (4.8 mg/100 mL), and color parameters between fresh and ultrasound treated samples at the studied temperatures. To predict the inactivation patterns, observed values were tested using 3 different general models: first-order, Weibull distribution, and biphasic. The Weibull and biphasic models show good correlation for inactivation under all processing conditions. Results show ultrasound in combination with mild temperature could be effectively used to process fresh carrot juice providing a safe product without affecting physicochemical characteristics. PRACTICAL APPLICATIONS: The combination of ultrasound and mild temperatures is effective in reducing microbial load in carrot juice to safe levels. This combination would be beneficial in the industrial processing of carrot juice without altering the quality attributes or bioactive compounds. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/28898409/Combined_Effect_of_Ultrasound_and_Mild_Temperatures_on_the_Inactivation_of_E__coli_in_Fresh_Carrot_Juice_and_Changes_on_its_Physicochemical_Characteristics_ DB - PRIME DP - Unbound Medicine ER -