Tags

Type your tag names separated by a space and hit enter

[Protective effects and mechanism of glycosides/phenol component of Moutan Cortex on renal injury of diabetic nephropathy rats].
Zhongguo Zhong Yao Za Zhi. 2016 Jun; 41(11):1990-1998.ZZ

Abstract

To evaluate the protective effects of glycosides/phenol component of Moutan Cortex (MC) on renal injury of diabetic nephropathy (DN) rats based on renal function parameters and histopathological examinations(HE staining and transmission electron microscope),and explore its possible mechanism by establishing DN rat models induced by high-sugar high-fat diet combined with streptozotocin (STZ). The results showed that compared with the model group, the MC glycosides/phenol component high and low dose groups(0.808, 0.404 g•kg⁻¹•d⁻¹) could significantly improve serum creatinine, blood urea nitrogen, urine protein and other abnormal renal function parameters. HE staining and transmission electron microscope results showed thickening of glomerular basement membrane, proliferation of mesangial cells and damages of podocyte structure in major rats of model group. However, the intervention of glycosides/phenol component of MC could effectively protect the glomerular injury. To explore its possible mechanism, the expressions of TGF-β1, fibronectin (FN) and collagen Ⅳ in renal tissues of rats in each group were detected by Western blot and immunohistochemical assay, and the phosphorylation levels of downstream effect factors (Smad2/3, p38MARK) of TGF-β1 were detected. The results showed that glycosides/phenol component of MC could effectively antagonize the activity of TGF-β1, lower the expressions of fibronectin (FN) and collagen Ⅳ inextracellular matrix (ECM), and resist against the thickening of glomerular basement membrane. More importantly, its protective effect on renal injury in DN rats may be associated with interfering the conduction of Smad, MARK pathways and resisting against the TGF-β1-induced ECM accumulation.

Authors+Show Affiliations

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. Third School of Clinical Medical, Nanjing University of Chinese Medicine, Nanjing 210028, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

28901091

Citation

Chen, Juan, et al. "[Protective Effects and Mechanism of Glycosides/phenol Component of Moutan Cortex On Renal Injury of Diabetic Nephropathy Rats]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 41, no. 11, 2016, pp. 1990-1998.
Chen J, Zhang MH, Zhang L, et al. [Protective effects and mechanism of glycosides/phenol component of Moutan Cortex on renal injury of diabetic nephropathy rats]. Zhongguo Zhong Yao Za Zhi. 2016;41(11):1990-1998.
Chen, J., Zhang, M. H., Zhang, L., Zhao, D., Feng, L., & Jia, X. B. (2016). [Protective effects and mechanism of glycosides/phenol component of Moutan Cortex on renal injury of diabetic nephropathy rats]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 41(11), 1990-1998. https://doi.org/10.4268/cjcmm20161104
Chen J, et al. [Protective Effects and Mechanism of Glycosides/phenol Component of Moutan Cortex On Renal Injury of Diabetic Nephropathy Rats]. Zhongguo Zhong Yao Za Zhi. 2016;41(11):1990-1998. PubMed PMID: 28901091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Protective effects and mechanism of glycosides/phenol component of Moutan Cortex on renal injury of diabetic nephropathy rats]. AU - Chen,Juan, AU - Zhang,Ming-Hua, AU - Zhang,Li, AU - Zhao,Di, AU - Feng,Liang, AU - Jia,Xiao-Bin, PY - 2015/12/22/received PY - 2017/9/14/entrez PY - 2016/6/1/pubmed PY - 2018/5/19/medline KW - Moutan Cortex KW - TGF-β1 KW - component KW - diabetic nephropathy (DN) KW - extracellular matrix (ECM) SP - 1990 EP - 1998 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 41 IS - 11 N2 - To evaluate the protective effects of glycosides/phenol component of Moutan Cortex (MC) on renal injury of diabetic nephropathy (DN) rats based on renal function parameters and histopathological examinations(HE staining and transmission electron microscope),and explore its possible mechanism by establishing DN rat models induced by high-sugar high-fat diet combined with streptozotocin (STZ). The results showed that compared with the model group, the MC glycosides/phenol component high and low dose groups(0.808, 0.404 g•kg⁻¹•d⁻¹) could significantly improve serum creatinine, blood urea nitrogen, urine protein and other abnormal renal function parameters. HE staining and transmission electron microscope results showed thickening of glomerular basement membrane, proliferation of mesangial cells and damages of podocyte structure in major rats of model group. However, the intervention of glycosides/phenol component of MC could effectively protect the glomerular injury. To explore its possible mechanism, the expressions of TGF-β1, fibronectin (FN) and collagen Ⅳ in renal tissues of rats in each group were detected by Western blot and immunohistochemical assay, and the phosphorylation levels of downstream effect factors (Smad2/3, p38MARK) of TGF-β1 were detected. The results showed that glycosides/phenol component of MC could effectively antagonize the activity of TGF-β1, lower the expressions of fibronectin (FN) and collagen Ⅳ inextracellular matrix (ECM), and resist against the thickening of glomerular basement membrane. More importantly, its protective effect on renal injury in DN rats may be associated with interfering the conduction of Smad, MARK pathways and resisting against the TGF-β1-induced ECM accumulation. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/28901091/[Protective_effects_and_mechanism_of_glycosides/phenol_component_of_Moutan_Cortex_on_renal_injury_of_diabetic_nephropathy_rats]_ L2 - https://medlineplus.gov/diabetickidneyproblems.html DB - PRIME DP - Unbound Medicine ER -