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Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae.
Plant Cell Rep. 2017 Nov; 36(11):1747-1755.PC

Abstract

KEY MESSAGE

This is the first report of stacking two major blast resistance genes in blast susceptible rice variety using co-transformation method to widen the resistance spectrum against different isolates of Magnaporthe oryzae. Single resistance (R-) gene mediated approach for the management of rice blast disease has met with frequent breakdown in resistance response. Besides providing the durable resistance, gene pyramiding or stacking also imparts broad spectrum resistance against plant pathogens, including rice blast. In the present study, we stacked two R-genes; Pi54 and Pi54rh having broad spectrum resistance against multiple isolates of Magnaporthe oryzae (M. oryzae). Both Pi54 and Pi54rh expressed under independent promoters were transferred into the blast susceptible japonica rice Taipei 309 (TP309) using particle gun bombardment method. Functional complementation analysis of stacked transgenic rice lines showed higher level of resistance to a set of highly virulent M. oryzae isolates collected from different rice growing regions. qRT-PCR analysis has shown M. oryzae induced expression of both the R-genes in stacked transgenic lines. The present study also demonstrated the effectiveness of the strategy for rapid single step gene stacking using co-transformation approach to engineer durable resistance against rice blast disease and also this is the first report in which two blast R-genes are stacked together using co-transformation approach. The two-gene-stacked transgenic line developed in this study can be used further to understand the molecular aspects of defense-related pathways vis-a-vis single R-gene containing transgenic lines.

Authors+Show Affiliations

ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India. Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India.ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.Crop Protection Section, Vivekananda Institute of Hill Agriculture, Almora, 263 601, Uttarakhand, India.Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012, India.Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India.National Agri-Food Biotechnology Institute (NABI), Mohali, Punjab, India. trsharma@nabi.res.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28905253

Citation

Kumari, Mandeep, et al. "Co-transformation Mediated Stacking of Blast Resistance Genes Pi54 and Pi54rh in Rice Provides Broad Spectrum Resistance Against Magnaporthe Oryzae." Plant Cell Reports, vol. 36, no. 11, 2017, pp. 1747-1755.
Kumari M, Rai AK, Devanna BN, et al. Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae. Plant Cell Rep. 2017;36(11):1747-1755.
Kumari, M., Rai, A. K., Devanna, B. N., Singh, P. K., Kapoor, R., Rajashekara, H., Prakash, G., Sharma, V., & Sharma, T. R. (2017). Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae. Plant Cell Reports, 36(11), 1747-1755. https://doi.org/10.1007/s00299-017-2189-x
Kumari M, et al. Co-transformation Mediated Stacking of Blast Resistance Genes Pi54 and Pi54rh in Rice Provides Broad Spectrum Resistance Against Magnaporthe Oryzae. Plant Cell Rep. 2017;36(11):1747-1755. PubMed PMID: 28905253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae. AU - Kumari,Mandeep, AU - Rai,Amit Kumar, AU - Devanna,B N, AU - Singh,Pankaj Kumar, AU - Kapoor,Ritu, AU - Rajashekara,H, AU - Prakash,G, AU - Sharma,Vinay, AU - Sharma,Tilak Raj, Y1 - 2017/09/13/ PY - 2017/05/29/received PY - 2017/07/27/accepted PY - 2017/9/15/pubmed PY - 2018/6/19/medline PY - 2017/9/15/entrez KW - Co-transformation KW - Gene stacking KW - Magnaporthe oryzae KW - Resistance gene KW - Rice blast SP - 1747 EP - 1755 JF - Plant cell reports JO - Plant Cell Rep VL - 36 IS - 11 N2 - KEY MESSAGE: This is the first report of stacking two major blast resistance genes in blast susceptible rice variety using co-transformation method to widen the resistance spectrum against different isolates of Magnaporthe oryzae. Single resistance (R-) gene mediated approach for the management of rice blast disease has met with frequent breakdown in resistance response. Besides providing the durable resistance, gene pyramiding or stacking also imparts broad spectrum resistance against plant pathogens, including rice blast. In the present study, we stacked two R-genes; Pi54 and Pi54rh having broad spectrum resistance against multiple isolates of Magnaporthe oryzae (M. oryzae). Both Pi54 and Pi54rh expressed under independent promoters were transferred into the blast susceptible japonica rice Taipei 309 (TP309) using particle gun bombardment method. Functional complementation analysis of stacked transgenic rice lines showed higher level of resistance to a set of highly virulent M. oryzae isolates collected from different rice growing regions. qRT-PCR analysis has shown M. oryzae induced expression of both the R-genes in stacked transgenic lines. The present study also demonstrated the effectiveness of the strategy for rapid single step gene stacking using co-transformation approach to engineer durable resistance against rice blast disease and also this is the first report in which two blast R-genes are stacked together using co-transformation approach. The two-gene-stacked transgenic line developed in this study can be used further to understand the molecular aspects of defense-related pathways vis-a-vis single R-gene containing transgenic lines. SN - 1432-203X UR - https://www.unboundmedicine.com/medline/citation/28905253/Co_transformation_mediated_stacking_of_blast_resistance_genes_Pi54_and_Pi54rh_in_rice_provides_broad_spectrum_resistance_against_Magnaporthe_oryzae_ DB - PRIME DP - Unbound Medicine ER -