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The analysis of 132 novel psychoactive substances in human hair using a single step extraction by tandem LC/MS.
Forensic Sci Int. 2017 Oct; 279:192-202.FS

Abstract

A rapid LC-MS/MS method for the targeted screening of 132 NPS in hair is described. Drugs include cathinones and synthetic cannabinoids, as well as amphetamine-type stimulants, piperazines and other hallucinogenic compounds. This method utilizes hair pulverization in acidified methanol followed by analysis using liquid chromatography coupled with tandem MS. The limit of detection varied from 0.001 to 0.1ng/mg hair among the various analytes. The method was validated in terms of sensitivity, selectivity, repeatability and stability. The limit of reporting was set at 0.1ng/mg of hair. The method was successfully applied to 23 medico-legal cases where NPS were detected in blood or where NPS use was suspected. The identified NPS included acetyl fentanyl, 25C-NBOMe, MDPV, PB-22 and AB-FUBINACA, allowing hair to be used where historical or retrospective information on use of NPS is sought. This technique has proven to be efficient for the one step extraction from hair of different classes of NPS in routine toxicological investigations; from unstable and volatile compounds, such as most of the cathinones, to hydrophobic compounds such as synthetic cannabinoids.

Authors+Show Affiliations

Department of Forensic Medicine, Monash University, Australia; Laboratory of Forensic Medicine & Toxicology, Faculty of Medicine, School of Health Sciences, University of Ioannina, Ioannina, Greece. Electronic address: vboumba@uoi.gr.Department of Forensic Medicine, Monash University, Australia; Victorian Institute of Forensic Medicine, 65 Kavanagh St., Southbank, Victoria 3006, Australia.Victorian Institute of Forensic Medicine, 65 Kavanagh St., Southbank, Victoria 3006, Australia.Department of Forensic Medicine, Monash University, Australia; Victorian Institute of Forensic Medicine, 65 Kavanagh St., Southbank, Victoria 3006, Australia.Department of Forensic Medicine, Monash University, Australia; Victorian Institute of Forensic Medicine, 65 Kavanagh St., Southbank, Victoria 3006, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28910664

Citation

Boumba, Vassiliki A., et al. "The Analysis of 132 Novel Psychoactive Substances in Human Hair Using a Single Step Extraction By Tandem LC/MS." Forensic Science International, vol. 279, 2017, pp. 192-202.
Boumba VA, Di Rago M, Peka M, et al. The analysis of 132 novel psychoactive substances in human hair using a single step extraction by tandem LC/MS. Forensic Sci Int. 2017;279:192-202.
Boumba, V. A., Di Rago, M., Peka, M., Drummer, O. H., & Gerostamoulos, D. (2017). The analysis of 132 novel psychoactive substances in human hair using a single step extraction by tandem LC/MS. Forensic Science International, 279, 192-202. https://doi.org/10.1016/j.forsciint.2017.08.031
Boumba VA, et al. The Analysis of 132 Novel Psychoactive Substances in Human Hair Using a Single Step Extraction By Tandem LC/MS. Forensic Sci Int. 2017;279:192-202. PubMed PMID: 28910664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The analysis of 132 novel psychoactive substances in human hair using a single step extraction by tandem LC/MS. AU - Boumba,Vassiliki A, AU - Di Rago,Matthew, AU - Peka,Melissa, AU - Drummer,Olaf H, AU - Gerostamoulos,Dimitri, Y1 - 2017/09/05/ PY - 2017/06/06/received PY - 2017/08/01/revised PY - 2017/08/29/accepted PY - 2017/9/15/pubmed PY - 2018/7/22/medline PY - 2017/9/15/entrez KW - Acidic extraction KW - Hair KW - LC–MS/MS KW - Novel psychoactive substances (NPS) KW - Screening SP - 192 EP - 202 JF - Forensic science international JO - Forensic Sci Int VL - 279 N2 - A rapid LC-MS/MS method for the targeted screening of 132 NPS in hair is described. Drugs include cathinones and synthetic cannabinoids, as well as amphetamine-type stimulants, piperazines and other hallucinogenic compounds. This method utilizes hair pulverization in acidified methanol followed by analysis using liquid chromatography coupled with tandem MS. The limit of detection varied from 0.001 to 0.1ng/mg hair among the various analytes. The method was validated in terms of sensitivity, selectivity, repeatability and stability. The limit of reporting was set at 0.1ng/mg of hair. The method was successfully applied to 23 medico-legal cases where NPS were detected in blood or where NPS use was suspected. The identified NPS included acetyl fentanyl, 25C-NBOMe, MDPV, PB-22 and AB-FUBINACA, allowing hair to be used where historical or retrospective information on use of NPS is sought. This technique has proven to be efficient for the one step extraction from hair of different classes of NPS in routine toxicological investigations; from unstable and volatile compounds, such as most of the cathinones, to hydrophobic compounds such as synthetic cannabinoids. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/28910664/The_analysis_of_132_novel_psychoactive_substances_in_human_hair_using_a_single_step_extraction_by_tandem_LC/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(17)30340-7 DB - PRIME DP - Unbound Medicine ER -