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Effects of short-term exposure to sevoflurane on the survival, proliferation, apoptosis, and differentiation of neural precursor cells derived from human embryonic stem cells.
J Anesth. 2017 Dec; 31(6):821-828.JA

Abstract

PURPOSE

Data from animal experiments suggest that exposure to general anesthetics in early life inhibits neurogenesis and causes long-term memory deficit. Considering short operating times and the popularity of sevoflurane in pediatric anesthesia, it is important to verify the effects of short-period exposure to sevoflurane on the developing brain.

METHODS

We measured the effects of short-term exposure (2 h) to 3%, 6%, or 8% sevoflurane, the most commonly used anesthetic, on neural precursor cells derived from human embryonic stem cells, SNUhES32. Cell survival, proliferation, apoptosis, and differentiation on days 1, 3, 5, and 7 post treatment were analyzed.

RESULTS

Treatment with 6% sevoflurane increased cell viability (P = 0.046) and decreased apoptosis (P = 0.014) on day 5, but the effect did not persist on day 7. Survival and apoptosis were not affected by 3% and 8% sevoflurane; there was no effect of proliferation at any of the tested concentrations. The differentiation of cells exposed to 6% or 8% sevoflurane decreased on day 1 (P = 0.033 and P = 0.036 for 6% and 8% sevoflurane, respectively) but was again normalized on days 3-7.

CONCLUSION

Clinically relevant treatment with sevoflurane for 2 h induces no significant changes in the survival, proliferation, apoptosis, and differentiation of human neural precursor cells, although supraclinical doses of sevoflurane do alter human neurogenesis transiently.

Authors+Show Affiliations

Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do, 13620, Republic of Korea.Research and Development Center, Jeil Pharmaceutical Co., Ltd, Yongin, Republic of Korea.Research and Development Center, Jeil Pharmaceutical Co., Ltd, Yongin, Republic of Korea.Research and Development Center, Jeil Pharmaceutical Co., Ltd, Yongin, Republic of Korea.Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do, 13620, Republic of Korea.Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do, 13620, Republic of Korea.Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do, 13620, Republic of Korea. anesing1@snu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28913662

Citation

Park, Jin-Woo, et al. "Effects of Short-term Exposure to Sevoflurane On the Survival, Proliferation, Apoptosis, and Differentiation of Neural Precursor Cells Derived From Human Embryonic Stem Cells." Journal of Anesthesia, vol. 31, no. 6, 2017, pp. 821-828.
Park JW, Lim MS, Ji SY, et al. Effects of short-term exposure to sevoflurane on the survival, proliferation, apoptosis, and differentiation of neural precursor cells derived from human embryonic stem cells. J Anesth. 2017;31(6):821-828.
Park, J. W., Lim, M. S., Ji, S. Y., Cho, M. S., Park, S. J., Han, S. H., & Kim, J. H. (2017). Effects of short-term exposure to sevoflurane on the survival, proliferation, apoptosis, and differentiation of neural precursor cells derived from human embryonic stem cells. Journal of Anesthesia, 31(6), 821-828. https://doi.org/10.1007/s00540-017-2408-1
Park JW, et al. Effects of Short-term Exposure to Sevoflurane On the Survival, Proliferation, Apoptosis, and Differentiation of Neural Precursor Cells Derived From Human Embryonic Stem Cells. J Anesth. 2017;31(6):821-828. PubMed PMID: 28913662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of short-term exposure to sevoflurane on the survival, proliferation, apoptosis, and differentiation of neural precursor cells derived from human embryonic stem cells. AU - Park,Jin-Woo, AU - Lim,Mi-Sun, AU - Ji,So Yeon, AU - Cho,Myung Soo, AU - Park,Seong-Joo, AU - Han,Sung-Hee, AU - Kim,Jin-Hee, Y1 - 2017/09/14/ PY - 2017/01/26/received PY - 2017/08/09/accepted PY - 2017/9/16/pubmed PY - 2018/5/2/medline PY - 2017/9/16/entrez KW - Anesthetics general KW - Human embryonic stem cells KW - Neurogenesis KW - Sevoflurane SP - 821 EP - 828 JF - Journal of anesthesia JO - J Anesth VL - 31 IS - 6 N2 - PURPOSE: Data from animal experiments suggest that exposure to general anesthetics in early life inhibits neurogenesis and causes long-term memory deficit. Considering short operating times and the popularity of sevoflurane in pediatric anesthesia, it is important to verify the effects of short-period exposure to sevoflurane on the developing brain. METHODS: We measured the effects of short-term exposure (2 h) to 3%, 6%, or 8% sevoflurane, the most commonly used anesthetic, on neural precursor cells derived from human embryonic stem cells, SNUhES32. Cell survival, proliferation, apoptosis, and differentiation on days 1, 3, 5, and 7 post treatment were analyzed. RESULTS: Treatment with 6% sevoflurane increased cell viability (P = 0.046) and decreased apoptosis (P = 0.014) on day 5, but the effect did not persist on day 7. Survival and apoptosis were not affected by 3% and 8% sevoflurane; there was no effect of proliferation at any of the tested concentrations. The differentiation of cells exposed to 6% or 8% sevoflurane decreased on day 1 (P = 0.033 and P = 0.036 for 6% and 8% sevoflurane, respectively) but was again normalized on days 3-7. CONCLUSION: Clinically relevant treatment with sevoflurane for 2 h induces no significant changes in the survival, proliferation, apoptosis, and differentiation of human neural precursor cells, although supraclinical doses of sevoflurane do alter human neurogenesis transiently. SN - 1438-8359 UR - https://www.unboundmedicine.com/medline/citation/28913662/Effects_of_short_term_exposure_to_sevoflurane_on_the_survival_proliferation_apoptosis_and_differentiation_of_neural_precursor_cells_derived_from_human_embryonic_stem_cells_ L2 - https://dx.doi.org/10.1007/s00540-017-2408-1 DB - PRIME DP - Unbound Medicine ER -