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A Metabolomic Approach Applied to a Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry Method (HPLC-ESI-HRMS/MS): Towards the Comprehensive Evaluation of the Chemical Composition of Cannabis Medicinal Extracts.
Phytochem Anal 2018; 29(2):144-155PA

Abstract

INTRODUCTION

Cannabis sativa L. is a powerful medicinal plant and its use has recently increased for the treatment of several pathologies. Nonetheless, side effects, like dizziness and hallucinations, and long-term effects concerning memory and cognition, can occur. Most alarming is the lack of a standardised procedure to extract medicinal cannabis. Indeed, each galenical preparation has an unknown chemical composition in terms of cannabinoids and other active principles that depends on the extraction procedure.

OBJECTIVE

This study aims to highlight the main differences in the chemical composition of Bediol® extracts when the extraction is carried out with either ethyl alcohol or olive oil for various times (0, 60, 120 and 180 min for ethyl alcohol, and 0, 60, 90 and 120 min for olive oil).

METHODOLOGY

Cannabis medicinal extracts (CMEs) were analysed by liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS) using an untargeted metabolomics approach. The data sets were processed by unsupervised multivariate analysis.

RESULTS

Our results suggested that the main difference lies in the ratio of acid to decarboxylated cannabinoids, which dramatically influences the pharmacological activity of CMEs. Minor cannabinoids, alkaloids, and amino acids contributing to this difference are also discussed. The main cannabinoids were quantified in each extract applying a recently validated LC-MS and LC-UV method.

CONCLUSIONS

Notwithstanding the use of a standardised starting plant material, great changes are caused by different extraction procedures. The metabolomics approach is a useful tool for the evaluation of the chemical composition of cannabis extracts. Copyright © 2017 John Wiley & Sons, Ltd.

Authors+Show Affiliations

Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via per Monteroni, 73100, Lecce, Italy. CNR NANOTEC, Campus Ecoteckne dell'Università del Salento, Via per Monteroni, 73100, Lecce, Italy.Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125, Modena, Italy. Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond, VA, 23298, USA.Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125, Modena, Italy.CNR NANOTEC, Campus Ecoteckne dell'Università del Salento, Via per Monteroni, 73100, Lecce, Italy. Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento & UdR INSTM di Lecce, c/o Campus Ecotekne, Via Monteroni, 73100, Lecce, Italy.Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Schubertstraβe 1, Graz, A-8010, Austria.Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125, Modena, Italy.CNR NANOTEC, Campus Ecoteckne dell'Università del Salento, Via per Monteroni, 73100, Lecce, Italy. Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125, Modena, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28915313

Citation

Citti, Cinzia, et al. "A Metabolomic Approach Applied to a Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry Method (HPLC-ESI-HRMS/MS): Towards the Comprehensive Evaluation of the Chemical Composition of Cannabis Medicinal Extracts." Phytochemical Analysis : PCA, vol. 29, no. 2, 2018, pp. 144-155.
Citti C, Battisti UM, Braghiroli D, et al. A Metabolomic Approach Applied to a Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry Method (HPLC-ESI-HRMS/MS): Towards the Comprehensive Evaluation of the Chemical Composition of Cannabis Medicinal Extracts. Phytochem Anal. 2018;29(2):144-155.
Citti, C., Battisti, U. M., Braghiroli, D., Ciccarella, G., Schmid, M., Vandelli, M. A., & Cannazza, G. (2018). A Metabolomic Approach Applied to a Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry Method (HPLC-ESI-HRMS/MS): Towards the Comprehensive Evaluation of the Chemical Composition of Cannabis Medicinal Extracts. Phytochemical Analysis : PCA, 29(2), pp. 144-155. doi:10.1002/pca.2722.
Citti C, et al. A Metabolomic Approach Applied to a Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry Method (HPLC-ESI-HRMS/MS): Towards the Comprehensive Evaluation of the Chemical Composition of Cannabis Medicinal Extracts. Phytochem Anal. 2018;29(2):144-155. PubMed PMID: 28915313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Metabolomic Approach Applied to a Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry Method (HPLC-ESI-HRMS/MS): Towards the Comprehensive Evaluation of the Chemical Composition of Cannabis Medicinal Extracts. AU - Citti,Cinzia, AU - Battisti,Umberto Maria, AU - Braghiroli,Daniela, AU - Ciccarella,Giuseppe, AU - Schmid,Martin, AU - Vandelli,Maria Angela, AU - Cannazza,Giuseppe, Y1 - 2017/09/15/ PY - 2017/05/25/received PY - 2017/07/21/revised PY - 2017/08/04/accepted PY - 2017/9/16/pubmed PY - 2018/8/17/medline PY - 2017/9/16/entrez KW - cannabinoids extraction KW - cannabis medicinal extracts KW - mass spectrometry KW - metabolomics SP - 144 EP - 155 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 29 IS - 2 N2 - INTRODUCTION: Cannabis sativa L. is a powerful medicinal plant and its use has recently increased for the treatment of several pathologies. Nonetheless, side effects, like dizziness and hallucinations, and long-term effects concerning memory and cognition, can occur. Most alarming is the lack of a standardised procedure to extract medicinal cannabis. Indeed, each galenical preparation has an unknown chemical composition in terms of cannabinoids and other active principles that depends on the extraction procedure. OBJECTIVE: This study aims to highlight the main differences in the chemical composition of Bediol® extracts when the extraction is carried out with either ethyl alcohol or olive oil for various times (0, 60, 120 and 180 min for ethyl alcohol, and 0, 60, 90 and 120 min for olive oil). METHODOLOGY: Cannabis medicinal extracts (CMEs) were analysed by liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS) using an untargeted metabolomics approach. The data sets were processed by unsupervised multivariate analysis. RESULTS: Our results suggested that the main difference lies in the ratio of acid to decarboxylated cannabinoids, which dramatically influences the pharmacological activity of CMEs. Minor cannabinoids, alkaloids, and amino acids contributing to this difference are also discussed. The main cannabinoids were quantified in each extract applying a recently validated LC-MS and LC-UV method. CONCLUSIONS: Notwithstanding the use of a standardised starting plant material, great changes are caused by different extraction procedures. The metabolomics approach is a useful tool for the evaluation of the chemical composition of cannabis extracts. Copyright © 2017 John Wiley & Sons, Ltd. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/28915313/A_Metabolomic_Approach_Applied_to_a_Liquid_Chromatography_Coupled_to_High_Resolution_Tandem_Mass_Spectrometry_Method__HPLC_ESI_HRMS/MS_:_Towards_the_Comprehensive_Evaluation_of_the_Chemical_Composition_of_Cannabis_Medicinal_Extracts_ L2 - https://doi.org/10.1002/pca.2722 DB - PRIME DP - Unbound Medicine ER -