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Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus.
BMC Complement Altern Med. 2017 Sep 16; 17(1):462.BC

Abstract

BACKGROUND

The emergence of multidrug-resistant bacteria is a worldwide concern and in order to find an alternative to this problem, the occurrence of antimicrobial compounds in Plectranthus amboinicus essential oil was investigated. Thus, this study aims to determine susceptibility of Staphylococcus aureus isolated from food to antibiotics, P. amboinicus essential oil (PAEO) and carvacrol.

METHODS

Leaves and stem of P. amboinicus were used for extraction of essential oil (PAEO) by hydrodistillation technique and EO chemical analysis was performed by gas chromatography coupled to a mass spectrometer. S. aureus strains (n = 35) isolated from food and S. aureus ATCC 6538 were used to evaluate the antimicrobial and antibiofilm activity of PAEO and carvacrol. All strains (n = 35) were submitted to antimicrobial susceptibility profile by disk diffusion method. Determination of MIC and MBC was performed by microdilution technique and antibiofilm activity was determined by microtiter-plate technique with crystal violet assay and counting viable cells in Colony Forming Units (CFU).

RESULTS

Carvacrol (88.17%) was the major component in the PAEO. Antibiotic resistance was detected in 28 S. aureus strains (80%) and 12 strains (34.3%) were oxacillin and vancomycin-resistant (OVRSA). From the 28 resistant strains, 7 (25%) showed resistance plasmid of 12,000 bp. All strains (n = 35) were sensitive to PAEO and carvacrol, with inhibition zones ranging from 16 to 38 mm and 23 to 42 mm, respectively. The lowest MIC (0.25 mg mL-1) and MBC (0.5 mg mL-1) values were observed when carvacrol was used against OVRSA. When a 0.5 mg mL-1 concentration of PAEO and carvacrol was used, no viable cells were found on S. aureus biofilm.

CONCLUSION

The antibacterial effect of carvacrol and PAEO proves to be a possible alternative against planktonic forms and staphylococcal biofilm.

Authors+Show Affiliations

Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil.Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil.Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil.Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil. State University of the Tocantina Region of Maranhão, Imperatriz, MA, 65901-480, Brazil.Chemistry Department, Federal Rural University of Rio de Janeiro, Seropédica-RJ, Rio de Janeiro, 23890-000, Brazil.Sea Sciences Institute, Federal University of Ceará, Fortaleza, CE, 60165-081, Brazil.Sea Sciences Institute, Federal University of Ceará, Fortaleza, CE, 60165-081, Brazil.Master's Program in Biotechnology, INTA College, Sobral, 62050-100, Brazil. renata.albuq@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28915875

Citation

Vasconcelos, Sara Edwirgens Costa Benício, et al. "Plectranthus Amboinicus Essential Oil and Carvacrol Bioactive Against Planktonic and Biofilm of Oxacillin- and Vancomycin-resistant Staphylococcus Aureus." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 462.
Vasconcelos SECB, Melo HM, Cavalcante TTA, et al. Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus. BMC Complement Altern Med. 2017;17(1):462.
Vasconcelos, S. E. C. B., Melo, H. M., Cavalcante, T. T. A., Júnior, F. E. A. C., de Carvalho, M. G., Menezes, F. G. R., de Sousa, O. V., & Costa, R. A. (2017). Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus. BMC Complementary and Alternative Medicine, 17(1), 462. https://doi.org/10.1186/s12906-017-1968-9
Vasconcelos SECB, et al. Plectranthus Amboinicus Essential Oil and Carvacrol Bioactive Against Planktonic and Biofilm of Oxacillin- and Vancomycin-resistant Staphylococcus Aureus. BMC Complement Altern Med. 2017 Sep 16;17(1):462. PubMed PMID: 28915875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus. AU - Vasconcelos,Sara Edwirgens Costa Benício, AU - Melo,Hider Machado, AU - Cavalcante,Theodora Thays Arruda, AU - Júnior,Francisco Eduardo Aragão Catunda, AU - de Carvalho,Mário Geraldo, AU - Menezes,Francisca Gleire Rodrigues, AU - de Sousa,Oscarina Viana, AU - Costa,Renata Albuquerque, Y1 - 2017/09/16/ PY - 2017/07/07/received PY - 2017/09/08/accepted PY - 2017/9/17/entrez PY - 2017/9/17/pubmed PY - 2017/10/17/medline KW - Drug-resistant bacteria KW - Plectranthus amboinicus KW - Staphylococcus aureus SP - 462 EP - 462 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: The emergence of multidrug-resistant bacteria is a worldwide concern and in order to find an alternative to this problem, the occurrence of antimicrobial compounds in Plectranthus amboinicus essential oil was investigated. Thus, this study aims to determine susceptibility of Staphylococcus aureus isolated from food to antibiotics, P. amboinicus essential oil (PAEO) and carvacrol. METHODS: Leaves and stem of P. amboinicus were used for extraction of essential oil (PAEO) by hydrodistillation technique and EO chemical analysis was performed by gas chromatography coupled to a mass spectrometer. S. aureus strains (n = 35) isolated from food and S. aureus ATCC 6538 were used to evaluate the antimicrobial and antibiofilm activity of PAEO and carvacrol. All strains (n = 35) were submitted to antimicrobial susceptibility profile by disk diffusion method. Determination of MIC and MBC was performed by microdilution technique and antibiofilm activity was determined by microtiter-plate technique with crystal violet assay and counting viable cells in Colony Forming Units (CFU). RESULTS: Carvacrol (88.17%) was the major component in the PAEO. Antibiotic resistance was detected in 28 S. aureus strains (80%) and 12 strains (34.3%) were oxacillin and vancomycin-resistant (OVRSA). From the 28 resistant strains, 7 (25%) showed resistance plasmid of 12,000 bp. All strains (n = 35) were sensitive to PAEO and carvacrol, with inhibition zones ranging from 16 to 38 mm and 23 to 42 mm, respectively. The lowest MIC (0.25 mg mL-1) and MBC (0.5 mg mL-1) values were observed when carvacrol was used against OVRSA. When a 0.5 mg mL-1 concentration of PAEO and carvacrol was used, no viable cells were found on S. aureus biofilm. CONCLUSION: The antibacterial effect of carvacrol and PAEO proves to be a possible alternative against planktonic forms and staphylococcal biofilm. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28915875/Plectranthus_amboinicus_essential_oil_and_carvacrol_bioactive_against_planktonic_and_biofilm_of_oxacillin__and_vancomycin_resistant_Staphylococcus_aureus_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1968-9 DB - PRIME DP - Unbound Medicine ER -