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Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants.
Microb Pathog. 2017 Oct; 111:375-387.MP

Abstract

Nanotechnology based therapeutics has emerged as a promising approach for augmenting the activity of existing antimicrobials due to the unique physical and chemical properties of nanoparticles (NPs). Nickel oxide nanoparticles (NiO-NPs) have been suggested as prospective antibacterial and antitumor agent. In this study, NiO-NPs have been synthesized by a green approach using Eucalyptus globulus leaf extract and assessed for their bactericidal activity. The morphology and purity of synthesized NiO-NPs determined through various spectroscopic techniques like UV-Visible, FT-IR, XRD, EDX and electron microscopy differed considerably. The synthesized NiO-NPs were pleomorphic varying in size between 10 and 20 nm. The XRD analysis revealed the average size of NiO-NPs as 19 nm. The UV-Vis spectroscopic data showed a strong SPR of NiO-NPs with a characteristic spectral peak at 396 nm. The FTIR data revealed various functional moieties like C=C, C-N, C-H and O-H which elucidate the role of leaf biomolecules in capping and dispersal of NiO-NPs. The bioactivity assay revealed the antibacterial and anti-biofilm activity of NiO-NPs against ESβL (+) E. coli, P. aeruginosa, methicillin sensitive and resistant S. aureus. Growth inhibition assay demonstrated time and NiO-NPs concentration dependent decrease in the viability of treated cells. NiO-NPs induced biofilm inhibition was revealed by a sharp increase in characteristic red fluorescence of PI, while SEM images of NiO-NPs treated cells were irregular shrink and distorted with obvious depressions/indentations. The results suggested significant antibacterial and antibiofilm activity of NiO-NPs which may play an important role in the management of infectious diseases affecting human health.

Authors+Show Affiliations

Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India. Electronic address: bilalahmed.amu@gmail.com.Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India; School of Biosciences and Biodiversity, Baba Ghulam Shah Badshah University, Rajouri, Jammu & Kashmir, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28916319

Citation

Saleem, Samia, et al. "Inhibition of Growth and Biofilm Formation of Clinical Bacterial Isolates By NiO Nanoparticles Synthesized From Eucalyptus Globulus Plants." Microbial Pathogenesis, vol. 111, 2017, pp. 375-387.
Saleem S, Ahmed B, Khan MS, et al. Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants. Microb Pathog. 2017;111:375-387.
Saleem, S., Ahmed, B., Khan, M. S., Al-Shaeri, M., & Musarrat, J. (2017). Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants. Microbial Pathogenesis, 111, 375-387. https://doi.org/10.1016/j.micpath.2017.09.019
Saleem S, et al. Inhibition of Growth and Biofilm Formation of Clinical Bacterial Isolates By NiO Nanoparticles Synthesized From Eucalyptus Globulus Plants. Microb Pathog. 2017;111:375-387. PubMed PMID: 28916319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants. AU - Saleem,Samia, AU - Ahmed,Bilal, AU - Khan,Mohammad Saghir, AU - Al-Shaeri,Majed, AU - Musarrat,Javed, Y1 - 2017/09/12/ PY - 2017/08/12/received PY - 2017/09/07/revised PY - 2017/09/11/accepted PY - 2017/9/17/pubmed PY - 2018/5/16/medline PY - 2017/9/17/entrez KW - Antibacterial KW - Antibiofilm KW - Eucalyptus leaf extract KW - Green synthesis KW - Nickel oxide nanoparticles SP - 375 EP - 387 JF - Microbial pathogenesis JO - Microb Pathog VL - 111 N2 - Nanotechnology based therapeutics has emerged as a promising approach for augmenting the activity of existing antimicrobials due to the unique physical and chemical properties of nanoparticles (NPs). Nickel oxide nanoparticles (NiO-NPs) have been suggested as prospective antibacterial and antitumor agent. In this study, NiO-NPs have been synthesized by a green approach using Eucalyptus globulus leaf extract and assessed for their bactericidal activity. The morphology and purity of synthesized NiO-NPs determined through various spectroscopic techniques like UV-Visible, FT-IR, XRD, EDX and electron microscopy differed considerably. The synthesized NiO-NPs were pleomorphic varying in size between 10 and 20 nm. The XRD analysis revealed the average size of NiO-NPs as 19 nm. The UV-Vis spectroscopic data showed a strong SPR of NiO-NPs with a characteristic spectral peak at 396 nm. The FTIR data revealed various functional moieties like C=C, C-N, C-H and O-H which elucidate the role of leaf biomolecules in capping and dispersal of NiO-NPs. The bioactivity assay revealed the antibacterial and anti-biofilm activity of NiO-NPs against ESβL (+) E. coli, P. aeruginosa, methicillin sensitive and resistant S. aureus. Growth inhibition assay demonstrated time and NiO-NPs concentration dependent decrease in the viability of treated cells. NiO-NPs induced biofilm inhibition was revealed by a sharp increase in characteristic red fluorescence of PI, while SEM images of NiO-NPs treated cells were irregular shrink and distorted with obvious depressions/indentations. The results suggested significant antibacterial and antibiofilm activity of NiO-NPs which may play an important role in the management of infectious diseases affecting human health. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/28916319/Inhibition_of_growth_and_biofilm_formation_of_clinical_bacterial_isolates_by_NiO_nanoparticles_synthesized_from_Eucalyptus_globulus_plants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(17)30997-X DB - PRIME DP - Unbound Medicine ER -