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Effects of the potential probiotics Bacillus aerophilus KADR3 in inducing immunity and disease resistance in Labeo rohita.
Fish Shellfish Immunol. 2017 Nov; 70:408-415.FS

Abstract

The aim of the present study was to evaluate the dietary administration of Bacillus aerophilus KADR3 on enhancing the immunity and disease resistance against Aeromonas hydrophila infection in Labeo rohita. B. aerophilus culture supernatant exhibited antagonistic activity against fish pathogenic bacteria in an agar well diffusion assay. Four fish groups were fed with either of following diets containing different concentrations of B. aerophilus KADR3 (cfu g-1): 0 (control), 1 × 107 (DI), 1 × 108 (DII), and 1 × 109 (DIII) -cfu g-1. Various innate immune parameters were measured at - 3rd and 6th - week post-feeding. At the end of 6th week, fish were challenged intraperitoneally with A. hydrophila and survival percentage was recorded over 10 days post-challenge. Studied immunological parameters viz. serum lysozyme, phagocytosis, serum total protein, respiratory bursts, serum IgM levels, superoxide dismutase and alternative complement pathway activities were significantly enhanced (P < 0.05) in fish groups fed with B. aerophilus KADR3 supplemented diets, with the highest values were observed in DII (108 cfu g-1) fed group. Further, B. aerophilus supplementation at 108 cfu g-1 exhibited highest post-challenge survivability i.e. 72.83%, followed by DIII (64.19%) and DI (41.97%). Our results collectively suggest that B. aerophilus can potentially be used as probiotic strain in aquaculture to enhance the immunity and disease resistance with an optimal dietary supplementation of 108 cfu g-1.

Authors+Show Affiliations

Department of Marine Biotechnology (NFMC), Bharathidasan University, Tiruchirapalli 620 024, Tamil Nadu, India. Electronic address: drammicro@gmail.com.Univ. Lille, CNRS, Univ. Littoral Cote d'Opale, UMR 8187, LOG, Laboratoire d'Océanologie et de Géosciences, 62930 Wimereux, France.Department of Marine Biotechnology (NFMC), Bharathidasan University, Tiruchirapalli 620 024, Tamil Nadu, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28917486

Citation

Ramesh, Dharmaraj, et al. "Effects of the Potential Probiotics Bacillus Aerophilus KADR3 in Inducing Immunity and Disease Resistance in Labeo Rohita." Fish & Shellfish Immunology, vol. 70, 2017, pp. 408-415.
Ramesh D, Souissi S, Ahamed TS. Effects of the potential probiotics Bacillus aerophilus KADR3 in inducing immunity and disease resistance in Labeo rohita. Fish Shellfish Immunol. 2017;70:408-415.
Ramesh, D., Souissi, S., & Ahamed, T. S. (2017). Effects of the potential probiotics Bacillus aerophilus KADR3 in inducing immunity and disease resistance in Labeo rohita. Fish & Shellfish Immunology, 70, 408-415. https://doi.org/10.1016/j.fsi.2017.09.037
Ramesh D, Souissi S, Ahamed TS. Effects of the Potential Probiotics Bacillus Aerophilus KADR3 in Inducing Immunity and Disease Resistance in Labeo Rohita. Fish Shellfish Immunol. 2017;70:408-415. PubMed PMID: 28917486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of the potential probiotics Bacillus aerophilus KADR3 in inducing immunity and disease resistance in Labeo rohita. AU - Ramesh,Dharmaraj, AU - Souissi,Sami, AU - Ahamed,Tharifkhan Shan, Y1 - 2017/09/14/ PY - 2017/03/20/received PY - 2017/09/11/revised PY - 2017/09/12/accepted PY - 2017/9/18/pubmed PY - 2018/6/26/medline PY - 2017/9/18/entrez KW - Aeromonas hydrophila KW - Bacillus aerophilus KADR3 KW - Cell free supernatant KW - Immune responses KW - Labeo rohita SP - 408 EP - 415 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 70 N2 - The aim of the present study was to evaluate the dietary administration of Bacillus aerophilus KADR3 on enhancing the immunity and disease resistance against Aeromonas hydrophila infection in Labeo rohita. B. aerophilus culture supernatant exhibited antagonistic activity against fish pathogenic bacteria in an agar well diffusion assay. Four fish groups were fed with either of following diets containing different concentrations of B. aerophilus KADR3 (cfu g-1): 0 (control), 1 × 107 (DI), 1 × 108 (DII), and 1 × 109 (DIII) -cfu g-1. Various innate immune parameters were measured at - 3rd and 6th - week post-feeding. At the end of 6th week, fish were challenged intraperitoneally with A. hydrophila and survival percentage was recorded over 10 days post-challenge. Studied immunological parameters viz. serum lysozyme, phagocytosis, serum total protein, respiratory bursts, serum IgM levels, superoxide dismutase and alternative complement pathway activities were significantly enhanced (P < 0.05) in fish groups fed with B. aerophilus KADR3 supplemented diets, with the highest values were observed in DII (108 cfu g-1) fed group. Further, B. aerophilus supplementation at 108 cfu g-1 exhibited highest post-challenge survivability i.e. 72.83%, followed by DIII (64.19%) and DI (41.97%). Our results collectively suggest that B. aerophilus can potentially be used as probiotic strain in aquaculture to enhance the immunity and disease resistance with an optimal dietary supplementation of 108 cfu g-1. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/28917486/Effects_of_the_potential_probiotics_Bacillus_aerophilus_KADR3_in_inducing_immunity_and_disease_resistance_in_Labeo_rohita_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(17)30556-9 DB - PRIME DP - Unbound Medicine ER -