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The effects of single or combined administration of galactooligosaccharide and Pediococcus acidilactici on cutaneous mucus immune parameters, humoral immune responses and immune related genes expression in common carp (Cyprinus carpio) fingerlings.
Fish Shellfish Immunol. 2017 Nov; 70:391-397.FS

Abstract

The present study was performed to investigate the effects of single or combined administration of galactooligosaccharide (GOS) and Pediococcus acidilactici on cutaneous mucus immune parameters, humoral immune responses and immune related genes expression in common carp (Cyprinus carpio) fingerlings. Carps were fed experimental diets for 8 weeks as follows: non-supplemented (Control), prebiotic diet (10 g/kg GOS), probiotic diet (1 g/kg [0.9 × 107 CFU] lyophilized P. acidilactici) and synbiotic diet (10 GOS in combination with 1 g/kg [0.9 × 107 CFU] lyophilized P. acidilactici). Unlike skin mucus, the serum lysozyme activity showed no significant difference between carps fed supplemented or control diets, however, remarkable elevation of serum ACH50 activity was noticed in carps fed supplemented diet (pro-, pre- and synbiotic diets) compared control group. Besides, feeding on pro-, pre- and synbiotic supplemented diets significantly increased serum and skin mucus total Ig levels. However, no significant difference was observed between treatments and control group in case of skin mucus proteases activity. There was no significant difference between expression levels of intestinal genes of LYZ and IL1b in fish fed on pre- and synbiotic, compared to the control. However, evaluation of TNF-alpha gene expression in the intestine of carps revealed remarkable down-regulation in treated groups (p < 0.05). These results indicated positive effect of supplementation of carp diet with GOS and P. acidilactici on some mucosal or serum immune parameters.

Authors+Show Affiliations

Aquatic Animal Health & Diseases Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: modanloo.maryam@gmail.com.Aquatic Animal Health & Diseases Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.Aquatic Animal Health & Diseases Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28917489

Citation

Modanloo, Maryam, et al. "The Effects of Single or Combined Administration of Galactooligosaccharide and Pediococcus Acidilactici On Cutaneous Mucus Immune Parameters, Humoral Immune Responses and Immune Related Genes Expression in Common Carp (Cyprinus Carpio) Fingerlings." Fish & Shellfish Immunology, vol. 70, 2017, pp. 391-397.
Modanloo M, Soltanian S, Akhlaghi M, et al. The effects of single or combined administration of galactooligosaccharide and Pediococcus acidilactici on cutaneous mucus immune parameters, humoral immune responses and immune related genes expression in common carp (Cyprinus carpio) fingerlings. Fish Shellfish Immunol. 2017;70:391-397.
Modanloo, M., Soltanian, S., Akhlaghi, M., & Hoseinifar, S. H. (2017). The effects of single or combined administration of galactooligosaccharide and Pediococcus acidilactici on cutaneous mucus immune parameters, humoral immune responses and immune related genes expression in common carp (Cyprinus carpio) fingerlings. Fish & Shellfish Immunology, 70, 391-397. https://doi.org/10.1016/j.fsi.2017.09.032
Modanloo M, et al. The Effects of Single or Combined Administration of Galactooligosaccharide and Pediococcus Acidilactici On Cutaneous Mucus Immune Parameters, Humoral Immune Responses and Immune Related Genes Expression in Common Carp (Cyprinus Carpio) Fingerlings. Fish Shellfish Immunol. 2017;70:391-397. PubMed PMID: 28917489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of single or combined administration of galactooligosaccharide and Pediococcus acidilactici on cutaneous mucus immune parameters, humoral immune responses and immune related genes expression in common carp (Cyprinus carpio) fingerlings. AU - Modanloo,Maryam, AU - Soltanian,Siyavash, AU - Akhlaghi,Mostafa, AU - Hoseinifar,Seyed Hossein, Y1 - 2017/09/13/ PY - 2017/06/29/received PY - 2017/09/07/revised PY - 2017/09/09/accepted PY - 2017/9/18/pubmed PY - 2018/6/26/medline PY - 2017/9/18/entrez KW - Common carp KW - Galactooligosaccharide KW - Gene expression KW - Mucosal immune parameters KW - Probiotic KW - Synbiotic SP - 391 EP - 397 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 70 N2 - The present study was performed to investigate the effects of single or combined administration of galactooligosaccharide (GOS) and Pediococcus acidilactici on cutaneous mucus immune parameters, humoral immune responses and immune related genes expression in common carp (Cyprinus carpio) fingerlings. Carps were fed experimental diets for 8 weeks as follows: non-supplemented (Control), prebiotic diet (10 g/kg GOS), probiotic diet (1 g/kg [0.9 × 107 CFU] lyophilized P. acidilactici) and synbiotic diet (10 GOS in combination with 1 g/kg [0.9 × 107 CFU] lyophilized P. acidilactici). Unlike skin mucus, the serum lysozyme activity showed no significant difference between carps fed supplemented or control diets, however, remarkable elevation of serum ACH50 activity was noticed in carps fed supplemented diet (pro-, pre- and synbiotic diets) compared control group. Besides, feeding on pro-, pre- and synbiotic supplemented diets significantly increased serum and skin mucus total Ig levels. However, no significant difference was observed between treatments and control group in case of skin mucus proteases activity. There was no significant difference between expression levels of intestinal genes of LYZ and IL1b in fish fed on pre- and synbiotic, compared to the control. However, evaluation of TNF-alpha gene expression in the intestine of carps revealed remarkable down-regulation in treated groups (p < 0.05). These results indicated positive effect of supplementation of carp diet with GOS and P. acidilactici on some mucosal or serum immune parameters. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/28917489/The_effects_of_single_or_combined_administration_of_galactooligosaccharide_and_Pediococcus_acidilactici_on_cutaneous_mucus_immune_parameters_humoral_immune_responses_and_immune_related_genes_expression_in_common_carp__Cyprinus_carpio__fingerlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(17)30551-X DB - PRIME DP - Unbound Medicine ER -