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Full validation of a method for the determination of drugs of abuse in non-mineralized dental biofilm using liquid chromatography-tandem mass spectrometry and application to postmortem samples.
Talanta. 2018 Jan 01; 176:360-366.T

Abstract

Alternative matrices play a major role in postmortem forensic toxicology, especially if common matrices (like body fluids or hair) are not available. Incorporation of illicit and medicinal drugs into non-mineralized dental biofilm (plaque) seems likely but has not been investigated so far. Analysis of plaque could therefore extend the spectrum of potentially used matrices in postmortem toxicology. For this reason, a rapid, simple and sensitive method for the extraction, determination and quantification of ten drugs of abuse from plaque using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and fully validated. Amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), 3,4-methylenedioxyamphetamine (MDA), cocaine, benzoylecgonine, morphine, codeine and 6-acetylmorphine were extracted from 2mg of dried and powdered plaque via ultrasonication with acetonitrile. The extracts were analyzed on a triple-quadrupole linear ion trap mass spectrometer in scheduled multiple reaction monitoring mode (sMRM). The method was fully validated and proved accurate, precise, selective and specific with satisfactory linearity within the calibrated ranges. The lower limit of quantification was 10-15pgmg-1 for all compounds except for MDA (100pgmg-1) and amphetamine (200pgmg-1). The method has been successfully applied to three authentic postmortem samples with known drug history. Amphetamine, MDMA, cocaine, benzoylecgonine, morphine and codeine could be detected in these cases in concentrations ranging from 18pgmg-1 for cocaine to 1400pgmg-1 for amphetamine.

Authors+Show Affiliations

Institute of Forensic Medicine, Forensic Toxicology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Albertstr. 9, 79104 Freiburg, Germany; Hermann Staudinger Graduate School, University of Freiburg, Hebelstr. 27, 79104 Freiburg, Germany.Center for Dental Medicine, Department of Operative Dentistry and Periodontology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany.Institute of Forensic Medicine, Forensic Toxicology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Albertstr. 9, 79104 Freiburg, Germany.Institute of Forensic Medicine, Forensic Toxicology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Albertstr. 9, 79104 Freiburg, Germany. Electronic address: merja.neukamm@uniklinik-freiburg.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28917762

Citation

Henkel, Kerstin, et al. "Full Validation of a Method for the Determination of Drugs of Abuse in Non-mineralized Dental Biofilm Using Liquid Chromatography-tandem Mass Spectrometry and Application to Postmortem Samples." Talanta, vol. 176, 2018, pp. 360-366.
Henkel K, Altenburger MJ, Auwärter V, et al. Full validation of a method for the determination of drugs of abuse in non-mineralized dental biofilm using liquid chromatography-tandem mass spectrometry and application to postmortem samples. Talanta. 2018;176:360-366.
Henkel, K., Altenburger, M. J., Auwärter, V., & Neukamm, M. A. (2018). Full validation of a method for the determination of drugs of abuse in non-mineralized dental biofilm using liquid chromatography-tandem mass spectrometry and application to postmortem samples. Talanta, 176, 360-366. https://doi.org/10.1016/j.talanta.2017.08.047
Henkel K, et al. Full Validation of a Method for the Determination of Drugs of Abuse in Non-mineralized Dental Biofilm Using Liquid Chromatography-tandem Mass Spectrometry and Application to Postmortem Samples. Talanta. 2018 Jan 1;176:360-366. PubMed PMID: 28917762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Full validation of a method for the determination of drugs of abuse in non-mineralized dental biofilm using liquid chromatography-tandem mass spectrometry and application to postmortem samples. AU - Henkel,Kerstin, AU - Altenburger,Markus J, AU - Auwärter,Volker, AU - Neukamm,Merja A, Y1 - 2017/08/15/ PY - 2017/02/14/received PY - 2017/08/09/revised PY - 2017/08/12/accepted PY - 2017/9/18/entrez PY - 2017/9/18/pubmed PY - 2018/9/7/medline KW - Alternative matrices KW - Forensic toxicology KW - LC-MS/MS KW - Plaque KW - Postmortem toxicology SP - 360 EP - 366 JF - Talanta JO - Talanta VL - 176 N2 - Alternative matrices play a major role in postmortem forensic toxicology, especially if common matrices (like body fluids or hair) are not available. Incorporation of illicit and medicinal drugs into non-mineralized dental biofilm (plaque) seems likely but has not been investigated so far. Analysis of plaque could therefore extend the spectrum of potentially used matrices in postmortem toxicology. For this reason, a rapid, simple and sensitive method for the extraction, determination and quantification of ten drugs of abuse from plaque using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and fully validated. Amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), 3,4-methylenedioxyamphetamine (MDA), cocaine, benzoylecgonine, morphine, codeine and 6-acetylmorphine were extracted from 2mg of dried and powdered plaque via ultrasonication with acetonitrile. The extracts were analyzed on a triple-quadrupole linear ion trap mass spectrometer in scheduled multiple reaction monitoring mode (sMRM). The method was fully validated and proved accurate, precise, selective and specific with satisfactory linearity within the calibrated ranges. The lower limit of quantification was 10-15pgmg-1 for all compounds except for MDA (100pgmg-1) and amphetamine (200pgmg-1). The method has been successfully applied to three authentic postmortem samples with known drug history. Amphetamine, MDMA, cocaine, benzoylecgonine, morphine and codeine could be detected in these cases in concentrations ranging from 18pgmg-1 for cocaine to 1400pgmg-1 for amphetamine. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/28917762/Full_validation_of_a_method_for_the_determination_of_drugs_of_abuse_in_non_mineralized_dental_biofilm_using_liquid_chromatography_tandem_mass_spectrometry_and_application_to_postmortem_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(17)30867-6 DB - PRIME DP - Unbound Medicine ER -