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Biofilms Benefiting Plants Exposed to ZnO and CuO Nanoparticles Studied with a Root-Mimetic Hollow Fiber Membrane.
J Agric Food Chem. 2018 Jul 05; 66(26):6619-6627.JA

Abstract

Plants exist with a consortium of microbes that influence plant health, including responses to biotic and abiotic stress. While nanoparticle (NP)-plant interactions are increasingly studied, the effect of NPs on the plant microbiome is less researched. Here a root-mimetic hollow fiber membrane (HFM) is presented for generating biofilms of plant-associated microbes nurtured by artificial root exudates (AREs) to correlate exudate composition with biofilm formation and response to NPs. Two microbial isolates from field-grown wheat, a bacillus endophyte and a pseudomonad root surface colonizer, were examined on HFMs fed with AREs varying in N and C composition. Bacterial morphology and biofilm architecture were characterized using scanning electron microscopy (SEM) and atomic force microscopy (AFM) and responses to CuO and ZnO NP challenges of 300 mg/L evaluated. The bacillus isolate sparsely colonized the HFM. In contrast, the pseudomonad formed robust biofilms within 3 days. Dependent on nutrient sources, the biofilm cells produced extensive extracellular polymeric substances (EPS) and large intracellular granules. Pseudomonad biofilms were minimally affected by ZnO NPs. CuO NPs, when introduced before biofilm maturation, strongly reduced biofilm formation. The findings demonstrate the utility of the HFM root-mimetic to study rhizoexudate influence on biofilms of root-colonizing microbes but without active plant metabolism. The results will allow better understanding of how microbe-rhizoexudate-NP interactions affect microbial and plant health.

Authors+Show Affiliations

Department of Biological Engineering , Utah State University , Logan , Utah 84322 , United States.Department of Biological Engineering , Utah State University , Logan , Utah 84322 , United States.Department of Biological Engineering , Utah State University , Logan , Utah 84322 , United States.Plants Soils Climate , Utah State University , Logan , Utah 84322 , United States.Utah Water Research Laboratory , Utah State University , Logan , Utah 84322 , United States.Department of Biology , Utah State University , Logan , Utah 84322 , United States.Department of Biological Engineering , Utah State University , Logan , Utah 84322 , United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28926236

Citation

Bonebrake, Michelle, et al. "Biofilms Benefiting Plants Exposed to ZnO and CuO Nanoparticles Studied With a Root-Mimetic Hollow Fiber Membrane." Journal of Agricultural and Food Chemistry, vol. 66, no. 26, 2018, pp. 6619-6627.
Bonebrake M, Anderson K, Valiente J, et al. Biofilms Benefiting Plants Exposed to ZnO and CuO Nanoparticles Studied with a Root-Mimetic Hollow Fiber Membrane. J Agric Food Chem. 2018;66(26):6619-6627.
Bonebrake, M., Anderson, K., Valiente, J., Jacobson, A., McLean, J. E., Anderson, A., & Britt, D. W. (2018). Biofilms Benefiting Plants Exposed to ZnO and CuO Nanoparticles Studied with a Root-Mimetic Hollow Fiber Membrane. Journal of Agricultural and Food Chemistry, 66(26), 6619-6627. https://doi.org/10.1021/acs.jafc.7b02524
Bonebrake M, et al. Biofilms Benefiting Plants Exposed to ZnO and CuO Nanoparticles Studied With a Root-Mimetic Hollow Fiber Membrane. J Agric Food Chem. 2018 Jul 5;66(26):6619-6627. PubMed PMID: 28926236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biofilms Benefiting Plants Exposed to ZnO and CuO Nanoparticles Studied with a Root-Mimetic Hollow Fiber Membrane. AU - Bonebrake,Michelle, AU - Anderson,Kaitlyn, AU - Valiente,Jonathan, AU - Jacobson,Astrid, AU - McLean,Joan E, AU - Anderson,Anne, AU - Britt,David W, Y1 - 2017/10/02/ PY - 2017/9/20/pubmed PY - 2018/7/14/medline PY - 2017/9/20/entrez KW - extracellular polymeric material KW - microbiome KW - nanoparticles KW - rhizosphere KW - root mimetic SP - 6619 EP - 6627 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 66 IS - 26 N2 - Plants exist with a consortium of microbes that influence plant health, including responses to biotic and abiotic stress. While nanoparticle (NP)-plant interactions are increasingly studied, the effect of NPs on the plant microbiome is less researched. Here a root-mimetic hollow fiber membrane (HFM) is presented for generating biofilms of plant-associated microbes nurtured by artificial root exudates (AREs) to correlate exudate composition with biofilm formation and response to NPs. Two microbial isolates from field-grown wheat, a bacillus endophyte and a pseudomonad root surface colonizer, were examined on HFMs fed with AREs varying in N and C composition. Bacterial morphology and biofilm architecture were characterized using scanning electron microscopy (SEM) and atomic force microscopy (AFM) and responses to CuO and ZnO NP challenges of 300 mg/L evaluated. The bacillus isolate sparsely colonized the HFM. In contrast, the pseudomonad formed robust biofilms within 3 days. Dependent on nutrient sources, the biofilm cells produced extensive extracellular polymeric substances (EPS) and large intracellular granules. Pseudomonad biofilms were minimally affected by ZnO NPs. CuO NPs, when introduced before biofilm maturation, strongly reduced biofilm formation. The findings demonstrate the utility of the HFM root-mimetic to study rhizoexudate influence on biofilms of root-colonizing microbes but without active plant metabolism. The results will allow better understanding of how microbe-rhizoexudate-NP interactions affect microbial and plant health. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/28926236/Biofilms_Benefiting_Plants_Exposed_to_ZnO_and_CuO_Nanoparticles_Studied_with_a_Root_Mimetic_Hollow_Fiber_Membrane_ L2 - https://doi.org/10.1021/acs.jafc.7b02524 DB - PRIME DP - Unbound Medicine ER -