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Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil.
Plant Biotechnol J. 2018 04; 16(4):926-938.PB

Abstract

Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring source plants. Thus, to further our understanding of metabolic bottlenecks that limit mFA accumulation, we generated transgenic Camelina sativa lines co-expressing Escherichia coli cyclopropane synthase (EcCPS) and Sterculia foetida lysophosphatidic acid acyltransferase (SfLPAT). In contrast to transgenic CPA-accumulating Arabidopsis, CPA accumulation in camelina caused only minor changes in seed weight, germination rate, oil accumulation and seedling development. CPA accumulated to much higher levels in membrane than storage lipids, comprising more than 60% of total fatty acid in both phosphatidylcholine (PC) and phosphatidylethanolamine (PE) versus 26% in diacylglycerol (DAG) and 12% in triacylglycerol (TAG) indicating bottlenecks in the transfer of CPA from PC to DAG and from DAG to TAG. Upon co-expression of SfLPAT with EcCPS, di-CPA-PC increased by ~50% relative to lines expressing EcCPS alone with the di-CPA-PC primarily observed in the embryonic axis and mono-CPA-PC primarily in cotyledon tissue. EcCPS-SfLPAT lines revealed a redistribution of CPA from the sn-1 to sn-2 positions within PC and PE that was associated with a doubling of CPA accumulation in both DAG and TAG. The identification of metabolic bottlenecks in acyl transfer between site of synthesis (phospholipids) and deposition in storage oils (TAGs) lays the foundation for the optimizing CPA accumulation through directed engineering of oil synthesis in target crops.

Authors+Show Affiliations

Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.Center for Plant Science Innovation, Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.Department of Biological Sciences, BioDiscovery Institute, University of North Texas, Denton, TX, USA.Biology Department, Brookhaven National Laboratory, Upton, NY, USA.Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.Biology Department, Brookhaven National Laboratory, Upton, NY, USA.Department of Biological Sciences, BioDiscovery Institute, University of North Texas, Denton, TX, USA.Center for Plant Science Innovation, Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA. Biology Department, Brookhaven National Laboratory, Upton, NY, USA.Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA. Biology Department, Brookhaven National Laboratory, Upton, NY, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

28929610

Citation

Yu, Xiao-Hong, et al. "Identification of Bottlenecks in the Accumulation of Cyclic Fatty Acids in Camelina Seed Oil." Plant Biotechnology Journal, vol. 16, no. 4, 2018, pp. 926-938.
Yu XH, Cahoon RE, Horn PJ, et al. Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil. Plant Biotechnol J. 2018;16(4):926-938.
Yu, X. H., Cahoon, R. E., Horn, P. J., Shi, H., Prakash, R. R., Cai, Y., Hearney, M., Chapman, K. D., Cahoon, E. B., Schwender, J., & Shanklin, J. (2018). Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil. Plant Biotechnology Journal, 16(4), 926-938. https://doi.org/10.1111/pbi.12839
Yu XH, et al. Identification of Bottlenecks in the Accumulation of Cyclic Fatty Acids in Camelina Seed Oil. Plant Biotechnol J. 2018;16(4):926-938. PubMed PMID: 28929610.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil. AU - Yu,Xiao-Hong, AU - Cahoon,Rebecca E, AU - Horn,Patrick J, AU - Shi,Hai, AU - Prakash,Richa R, AU - Cai,Yuanheng, AU - Hearney,Maegan, AU - Chapman,Kent D, AU - Cahoon,Edgar B, AU - Schwender,Jorg, AU - Shanklin,John, Y1 - 2018/01/18/ PY - 2017/07/10/received PY - 2017/09/01/revised PY - 2017/09/14/accepted PY - 2017/9/21/pubmed PY - 2018/12/29/medline PY - 2017/9/21/entrez KW - Camelina sativa KW - cyclopropane fatty acid KW - lipid metabolism KW - lipid synthesis KW - triacylglycerol KW - unusual fatty acid SP - 926 EP - 938 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 16 IS - 4 N2 - Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring source plants. Thus, to further our understanding of metabolic bottlenecks that limit mFA accumulation, we generated transgenic Camelina sativa lines co-expressing Escherichia coli cyclopropane synthase (EcCPS) and Sterculia foetida lysophosphatidic acid acyltransferase (SfLPAT). In contrast to transgenic CPA-accumulating Arabidopsis, CPA accumulation in camelina caused only minor changes in seed weight, germination rate, oil accumulation and seedling development. CPA accumulated to much higher levels in membrane than storage lipids, comprising more than 60% of total fatty acid in both phosphatidylcholine (PC) and phosphatidylethanolamine (PE) versus 26% in diacylglycerol (DAG) and 12% in triacylglycerol (TAG) indicating bottlenecks in the transfer of CPA from PC to DAG and from DAG to TAG. Upon co-expression of SfLPAT with EcCPS, di-CPA-PC increased by ~50% relative to lines expressing EcCPS alone with the di-CPA-PC primarily observed in the embryonic axis and mono-CPA-PC primarily in cotyledon tissue. EcCPS-SfLPAT lines revealed a redistribution of CPA from the sn-1 to sn-2 positions within PC and PE that was associated with a doubling of CPA accumulation in both DAG and TAG. The identification of metabolic bottlenecks in acyl transfer between site of synthesis (phospholipids) and deposition in storage oils (TAGs) lays the foundation for the optimizing CPA accumulation through directed engineering of oil synthesis in target crops. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/28929610/Identification_of_bottlenecks_in_the_accumulation_of_cyclic_fatty_acids_in_camelina_seed_oil_ L2 - https://doi.org/10.1111/pbi.12839 DB - PRIME DP - Unbound Medicine ER -