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Methane-metabolizing microbial communities in sediments of the Haima cold seep area, northwest slope of the South China Sea.
FEMS Microbiol Ecol 2017; 93(9)FM

Abstract

Cold seeps are widespread chemosynthetic ecosystems in the deep-sea environment, and cold seep microbial communities of the South China Sea are poorly constrained. Here we report on the archaeal communities, particularly those involved in methane metabolization, in sediments of a newly discovered cold seep (named 'Haima') on the northwest slope of the South China Sea. Archaeal diversity, abundance and distribution were investigated in two piston cores collected from a seep area (QDN-14B) and a non-seep control site (QDN-31B). Geochemical investigation of the QDN-14B core identified an estimated sulfate-methane transition zone (Estimated SMTZ) at 300-400 cm below sea floor (cmbsf), where a high abundance of anaerobic methane-oxidizing archaea (ANME) occurred, as revealed by analysis of the 16S rRNA gene and the gene (mcrA) encoding the α-subunit of the key enzyme methyl-coenzyme M reductase. ANME-2a/b was predominant in the upper and middle layers of the estimated SMTZ, whereas ANME-1b outcompeted ANME-2 in the sulfate-depleted bottom layers of the estimated SMTZ and the methanogenic zone. Fine-scale phylogenetic analysis further divided the ANME-1b group into three subgroups with different distribution patterns: ANME-1bI, ANME-1bII and ANME-1bIII. Multivariate analyses indicated that dissolved inorganic carbon and sulfate may be important factors controlling the composition of the methane-metabolizing community. Our study on ANME niche separation and interactions with other archaeal groups improves our understanding of the metabolic diversity and flexibility of ANME, and the findings further suggest that ANME subgroups may have evolved diversified/specified metabolic capabilities other than syntrophic anaerobic oxidation of methane coupled with sulfate reduction in marine sediments.

Authors+Show Affiliations

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.Department of Marine Sciences, University of Georgia, Athens, GA, 30602, USA.Guangzhou Marine Geological Survey, China Geological Survey, Guangzhou, 510740, China.State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28934399

Citation

Niu, Mingyang, et al. "Methane-metabolizing Microbial Communities in Sediments of the Haima Cold Seep Area, Northwest Slope of the South China Sea." FEMS Microbiology Ecology, vol. 93, no. 9, 2017.
Niu M, Fan X, Zhuang G, et al. Methane-metabolizing microbial communities in sediments of the Haima cold seep area, northwest slope of the South China Sea. FEMS Microbiol Ecol. 2017;93(9).
Niu, M., Fan, X., Zhuang, G., Liang, Q., & Wang, F. (2017). Methane-metabolizing microbial communities in sediments of the Haima cold seep area, northwest slope of the South China Sea. FEMS Microbiology Ecology, 93(9), doi:10.1093/femsec/fix101.
Niu M, et al. Methane-metabolizing Microbial Communities in Sediments of the Haima Cold Seep Area, Northwest Slope of the South China Sea. FEMS Microbiol Ecol. 2017 09 1;93(9) PubMed PMID: 28934399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methane-metabolizing microbial communities in sediments of the Haima cold seep area, northwest slope of the South China Sea. AU - Niu,Mingyang, AU - Fan,Xibei, AU - Zhuang,Guangchao, AU - Liang,Qianyong, AU - Wang,Fengping, PY - 2017/03/03/received PY - 2017/08/09/accepted PY - 2017/9/22/entrez PY - 2017/9/22/pubmed PY - 2018/1/5/medline KW - ANME KW - South China Sea KW - cold seep KW - diversity KW - habitat segregation KW - methanogens JF - FEMS microbiology ecology JO - FEMS Microbiol. Ecol. VL - 93 IS - 9 N2 - Cold seeps are widespread chemosynthetic ecosystems in the deep-sea environment, and cold seep microbial communities of the South China Sea are poorly constrained. Here we report on the archaeal communities, particularly those involved in methane metabolization, in sediments of a newly discovered cold seep (named 'Haima') on the northwest slope of the South China Sea. Archaeal diversity, abundance and distribution were investigated in two piston cores collected from a seep area (QDN-14B) and a non-seep control site (QDN-31B). Geochemical investigation of the QDN-14B core identified an estimated sulfate-methane transition zone (Estimated SMTZ) at 300-400 cm below sea floor (cmbsf), where a high abundance of anaerobic methane-oxidizing archaea (ANME) occurred, as revealed by analysis of the 16S rRNA gene and the gene (mcrA) encoding the α-subunit of the key enzyme methyl-coenzyme M reductase. ANME-2a/b was predominant in the upper and middle layers of the estimated SMTZ, whereas ANME-1b outcompeted ANME-2 in the sulfate-depleted bottom layers of the estimated SMTZ and the methanogenic zone. Fine-scale phylogenetic analysis further divided the ANME-1b group into three subgroups with different distribution patterns: ANME-1bI, ANME-1bII and ANME-1bIII. Multivariate analyses indicated that dissolved inorganic carbon and sulfate may be important factors controlling the composition of the methane-metabolizing community. Our study on ANME niche separation and interactions with other archaeal groups improves our understanding of the metabolic diversity and flexibility of ANME, and the findings further suggest that ANME subgroups may have evolved diversified/specified metabolic capabilities other than syntrophic anaerobic oxidation of methane coupled with sulfate reduction in marine sediments. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/28934399/Methane_metabolizing_microbial_communities_in_sediments_of_the_Haima_cold_seep_area_northwest_slope_of_the_South_China_Sea_ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1093/femsec/fix101 DB - PRIME DP - Unbound Medicine ER -